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. 1979 Jan;15(1):59–66. doi: 10.1128/aac.15.1.59

Effects of Carbon Sources on Antibiotic Resistance in Pseudomonas aeruginosa

Robert S Conrad 1, R Gary Wulf 1, Damonda L Clay 1
PMCID: PMC352601  PMID: 106771

Abstract

The metabolism of branched-chain amino acids, branched-chain acyl derivatives, d-glucose, l-glutamate, and Mueller-Hinton medium was investigated to determine their effects on the growth, lipid composition, and antibiotic susceptibility of Pseudomonas aeruginosa. The unsaturated fatty acid content of the readily extractable lipids was altered by growth on selected branched-chain amino acids and their acyl derivatives. Bacteria grown on branched-chain acyl derivatives became more susceptible to polymyxin B and colistin. The effect acyl derivatives had on increasing susceptibility was also manifest in mixed media which contained both an acyl derivative and a carbon source which did not increase susceptibility. Growth on branched-chain amino acids gave mixed results which were dependent on a number of factors, including unique manifestations of individual amino acids, growth conditions, and availability of other carbon sources. The cultural conditions which altered susceptibility to polymyxin antibiotics did not correlate with similar effects on susceptibility to carbenicillin and gentamicin. An adaptive resistance to polymyxin B was observed when the sole carbon source was d-glucose or l-glutamate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  2. Brown M. R., Wood S. M. Relation between cation and lipid content of cell walls of Pseudomonas aeruginosa, Proteus vulgaris and Klebsiella aerogenes and their sensitivity to polymyxin B and other antibacterial agents. J Pharm Pharmacol. 1972 Mar;24(3):215–218. doi: 10.1111/j.2042-7158.1972.tb08967.x. [DOI] [PubMed] [Google Scholar]
  3. Dunnick J. K., O'Leary W. M. Correlation of bacteria lipid composition with antibiotic resistance. J Bacteriol. 1970 Mar;101(3):892–900. doi: 10.1128/jb.101.3.892-900.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edmonds P., Cooney J. J. Lipids of Pseudomonas aeruginosa cells grown on hydrocarbons and on trypticase soy broth. J Bacteriol. 1969 Apr;98(1):16–22. doi: 10.1128/jb.98.1.16-22.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  6. Farrell J., Rose A. Temperature effects on microorganisms. Annu Rev Microbiol. 1967;21:101–120. doi: 10.1146/annurev.mi.21.100167.000533. [DOI] [PubMed] [Google Scholar]
  7. Kenward M. A., Brown M. R., Hesslewood S. R., Dillon C. Influence of R-plasmid RP1 of Pseudomonas aeruginosa on cell wall composition, drug resistance, and sensitivity to cold shock. Antimicrob Agents Chemother. 1978 Mar;13(3):446–453. doi: 10.1128/aac.13.3.446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MORRISON W. R., SMITH L. M. PREPARATION OF FATTY ACID METHYL ESTERS AND DIMETHYLACETALS FROM LIPIDS WITH BORON FLUORIDE--METHANOL. J Lipid Res. 1964 Oct;5:600–608. [PubMed] [Google Scholar]
  9. Massey L. K., Sokatch J. R., Conrad R. S. Branched-chain amino acid catabolism in bacteria. Bacteriol Rev. 1976 Mar;40(1):42–54. doi: 10.1128/br.40.1.42-54.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ornston L. N. Regulation of catabolic pathways in Pseudomonas. Bacteriol Rev. 1971 Jun;35(2):87–116. doi: 10.1128/br.35.2.87-116.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Roe E., Jones R. J., Lowbury E. J. Transfer of anibioic resistanceetween Pseudomonas aeruginosa, Escherichia coli, and other gram-negative bacilli in rns. Lancet. 1971 Jan 23;1(7691):149–152. doi: 10.1016/s0140-6736(71)91930-1. [DOI] [PubMed] [Google Scholar]
  12. Teuber M., Miller I. R. Selective binding of polymyxin B to negatively charged lipid monolayers. Biochim Biophys Acta. 1977 Jun 16;467(3):280–289. doi: 10.1016/0005-2736(77)90305-4. [DOI] [PubMed] [Google Scholar]

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