Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1979 Oct;16(4):452–457. doi: 10.1128/aac.16.4.452

Agar medium for use in susceptibility testing of bacteria from human periodontal pockets.

C B Walker, T A Niebloom, S S Socransky
PMCID: PMC352881  PMID: 518074

Abstract

An agar medium (medium V) was formulated to determine the minimal inhibitory concentrations (MICs) of antimicrobial agents for bacteria encountered in human periodontal pockets. The medium contained (per liter) Trypticase, 15 g; yeast extract, 5 g; sodium chloride, 5 g; glucose, 2 g; sodium pyruvate, 2 g; sodium formate, 1 g; sodium fumarate, 1.5 g; sodium succinate, 0.1 g; Tween 80, 0.25 ml; agar, 15 g; hemin, 5 mg; and menadione, 0.5 mg. The growth of 50 oral strains was compared on this and six other media which included: Wilkins-Chalgren agar, Schaedler agar, Brucella agar, Trypticase-soy blood agar, and Schaedler and Brucella agars supplemented with whole blood. Growth, for most strains, was greatest on medium V. Medium V was also compared with Wilkins-Chalgren agar, using the same oral strains, to determine the MICs of the following antibiotics: penicillin, tetracycline, chloramphenicol, clindamycin, and erythromycin. The MICs of these antibiotics were essentially the same on both media when growth was quantitatively similar.

Full text

PDF
456

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. GIBBONS R. J., MACDONALD J. B. Hemin and vitamin K compounds as required factors for the cultivation of certain strains of Bacteroides melaninogenicus. J Bacteriol. 1960 Aug;80:164–170. doi: 10.1128/jb.80.2.164-170.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Lev M., Keudell K. C., Milford A. F. Succinate as a growth factor for Bacteroides melaninogenicus. J Bacteriol. 1971 Oct;108(1):175–178. doi: 10.1128/jb.108.1.175-178.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Listgarten M. A., Lindhe J., Hellden L. Effect of tetracycline and/or scaling on human periodontal disease. Clinical, microbiological, and histological observations. J Clin Periodontol. 1978 Nov;5(4):246–271. doi: 10.1111/j.1600-051x.1978.tb01918.x. [DOI] [PubMed] [Google Scholar]
  4. Listgarten M. A., Lindhe J., Parodi R. The effect of systemic antimicrobial therapy on plaque and gingivitis in dogs. J Periodontal Res. 1979 Jan;14(1):65–75. doi: 10.1111/j.1600-0765.1979.tb00219.x. [DOI] [PubMed] [Google Scholar]
  5. Murray P. R. Growth of clinical isolates of anaerobic bacteria on agar media: effects of media composition, storage conditions, and reduction under anaerobic conditions. J Clin Microbiol. 1978 Dec;8(6):708–714. doi: 10.1128/jcm.8.6.708-714.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Prevention of major dental disorders. Symposium held at Marabou, Sundbyberg, Sweden, June 16, 1979. J Clin Periodontol. 1979 Dec;6(7):1–89. [PubMed] [Google Scholar]
  7. QUINTO G., SEBALD II. IDENTIFICATION OF THREE HEMIN-REQUIRING BACTEROIDES STRAINS. Am J Med Technol. 1964 Nov-Dec;30:381–384. [PubMed] [Google Scholar]
  8. SCHAEDLER R. W., DUBOS R., COSTELLO R. THE DEVELOPMENT OF THE BACTERIAL FLORA IN THE GASTROINTESTINAL TRACT OF MICE. J Exp Med. 1965 Jul 1;122:59–66. doi: 10.1084/jem.122.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Smibert R. M., Holdeman L. V. Clinical isolates of anaerobic gram-negative rods with a formate-fumarate energy metabolism: Bacteroides corrodens, Vibrio succionogenes, and unidentified strains. J Clin Microbiol. 1976 Apr;3(4):432–437. doi: 10.1128/jcm.3.4.432-437.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Stalons D. R., Thornsberry C., Dowell V. R., Jr Effect of culture medium and carbon dioxide concentration on growth of anaerobic bacteria commonly encountered in clinical specimens. Appl Microbiol. 1974 Jun;27(6):1098–1104. doi: 10.1128/am.27.6.1098-1104.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Starr S. E., Killgore G. E., Dowell V. R., Jr Comparison of Schaedler agar and trypticase soy-yeast extract agar for the cultivation of anaerobic bacteria. Appl Microbiol. 1971 Oct;22(4):655–658. doi: 10.1128/am.22.4.655-658.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wilkins T. D., Chalgren S. Medium for use in antibiotic susceptibility testing of anaerobic bacteria. Antimicrob Agents Chemother. 1976 Dec;10(6):926–928. doi: 10.1128/aac.10.6.926. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wilkins T. D., Walker C. B. Development of a micromethod for identification of anaerobic bacteria. Appl Microbiol. 1975 Nov;30(5):825–830. doi: 10.1128/am.30.5.825-830.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES