Abstract
A total of 222 clinical isolates were used to test the accuracy of Sensititre dried microtitration trays for determining minimum inhibitory concentrations (MICs) of antimicrobial agents. In comparison with an agar dilution technique, 89.5% of all the pairs of results were within one doubling dilution. The 2,420 pairs of MIC results with finite values gave a corresponding figure of 86.8%. Exclusion of sulfisoxazole results, which demonstrated a significant interlaboratory variation in accuracy, raised this value to 89.1%. Very good differentiation of beta-lactamase-producing strains of Staphylococcus aureus (24 of 24 giving an MIC greater than or equal to 0.25 micrograms/ml) and Haemophilus influenzae (3 of 3 giving an MIC greater than or equal to 32 micrograms/ml) was obtained with the Sensititre system. This method also clearly distinguished erythromycin-resistant S. aureus strains (7 of 7 giving an MIC greater than 32 micrograms/ml) from the susceptible strains (26 of 28 giving an MIC less than or equal to 0.5 microgram/ml plus 1 strain at 1.0 microgram/ml and 1 at 2.0 micrograms/ml). Sensititre offers an accurate and convenient method of determining MICs comparable to those obtained with the agar dilution procedure, with the advantage of an extended shelf life when stored at room temperature.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aswapokee N., Neu H. C. In vitro activity and beta-lactamase stability of cefazaflur compared with those of beta-lactamase-stable cephalosporins. Antimicrob Agents Chemother. 1979 Mar;15(3):444–446. doi: 10.1128/aac.15.3.444. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barry A. L., Jones R. N., Gavan T. L. Evaluation of the micro-media system for quantitative antimicrobial drug susceptibility testing: a collaborative study. Antimicrob Agents Chemother. 1978 Jan;13(1):61–69. doi: 10.1128/aac.13.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Garrod L. P., Waterworth P. M. Effect of medium composition on the apparent sensitivity of Pseudomonas aeruginosa to gentamicin. J Clin Pathol. 1969 Sep;22(5):534–538. doi: 10.1136/jcp.22.5.534. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gavan T. L., Jones R. N., Barry A. L. Evaluation of the Sensititre system for quantitative antimicrobial drug susceptibility testing: a collaborative study. Antimicrob Agents Chemother. 1980 Mar;17(3):464–469. doi: 10.1128/aac.17.3.464. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hamilton-Miller J. M. Towards greater uniformity in sensitivity testing. J Antimicrob Chemother. 1977 Sep;3(5):385–392. doi: 10.1093/jac/3.5.385. [DOI] [PubMed] [Google Scholar]
- Jacobs M. R., Mithal Y., Robins-Browne R. M., Gaspar M. N., Koornhof H. J. Antimicrobial susceptibility testing of pneumococci: determination of Kirby-Bauer breakpoints for penicillin G, erythromycin, clindamycin, tetracycline, chloramphenicol, and rifampin. Antimicrob Agents Chemother. 1979 Aug;16(2):190–197. doi: 10.1128/aac.16.2.190. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McMaster P. R., Robertson E. A., Witebsky F. G., MacLowry J. D. Evaluation of a dispensing instrument (Dynatech MIC-2000) for preparing microtiter antibiotic plates and testing their potency during storage. Antimicrob Agents Chemother. 1978 May;13(5):842–844. doi: 10.1128/aac.13.5.842. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Callaghan C. H., Morris A., Kirby S. M., Shingler A. H. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1(4):283–288. doi: 10.1128/aac.1.4.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Phillips I., Eykyn S., King B. A., Jenkins C., Warren C. A., Shannon K. P. The in vitro antibacterial activity of nine aminoglycosides and spectinomycin on clinical isolates of common Gram-negative bacteria. J Antimicrob Chemother. 1977 Sep;3(5):403–410. doi: 10.1093/jac/3.5.403. [DOI] [PubMed] [Google Scholar]
- Wise R., Andrews J. M., Bedford K. A. LY127935, a novel oxa-beta-lactam: an in vitro comparison with other beta-lactam antibiotics. Antimicrob Agents Chemother. 1979 Sep;16(3):341–345. doi: 10.1128/aac.16.3.341. [DOI] [PMC free article] [PubMed] [Google Scholar]