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. 1979 Jan;29(1):69–75. doi: 10.1128/jvi.29.1.69-75.1979

Relationship between T-antigen and tumor-specific transplantation antigen in simian virus 40-transformed cells.

C Chang, R G Martin, D M Livingston, S W Luborsky, C P Hu, P T Mora
PMCID: PMC353074  PMID: 219235

Abstract

The simian virus 40 (sv40) tumor antigen (T-antigen) and tumor-specific transplantation antigen (TSTA) have been partially purified and studied to clarify their relationship. The T-antigen and the TSTA were partially purified from nuclei of SV AL/N cells, and SV40-transformed mouse embryo fibroblast line, by precipitation with ammonium sulfate and chromatography on DEAE- and DNA-cellulose. The T-antigen was assayed by complement fixation, and the TSTA was assayed by its ability to immunize mice against SV40-containing ascites tumor cells. When T-antigen- and TSTA-containing preparations were sedimented through sucrose gradients, each antigen had a major peak of activity at a sedimentation coefficient of 6.7 and minor peaks in other regions. Antiserum against T-antigen (from tumor-bearing hamsters) immunoprecipitated the TSTA activity. A preparation of T-antigen from human SV80 cells, which exhibited only one protein band after sodium dodecylsulfate-polyacrylamide gel electrophoresis, had TSTA activity when as little as 0.6 microgram of protein per mouse was used for immunization. These experiments demonstrate that the T-antigen, the product of the SV40 early A gene is capable of inducing specific immunity against transplantation of SV40-transformed tumor cells in mice.

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Selected References

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  1. Ahmad-Zadeh C., Allet B., Greenblatt J., Weil R. Two forms of simian-virus-40-specific T-antigen in abortive and lytic infection. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1097–1101. doi: 10.1073/pnas.73.4.1097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson J. L., Chang C., Mora P. T., Martin R. G. Expression and thermal stability of simian virus 40 tumor-specific transplantation antigen and tumor antigen in wild type- and tsA mutant-transformed cells. J Virol. 1977 Feb;21(2):459–467. doi: 10.1128/jvi.21.2.459-467.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Anderson J. L., Martin R. G., Chang C., Mora P. T., Livingston D. M. Nuclear preparations of SV40-transformed cells contain tumor-specific transplantation antigen activity. Virology. 1977 Jan;76(1):420–425. doi: 10.1016/0042-6822(77)90314-2. [DOI] [PubMed] [Google Scholar]
  4. Brugge J. S., Butel J. S. Role of simian virus 40 gene A function in maintenance of transformation. J Virol. 1975 Mar;15(3):619–635. doi: 10.1128/jvi.15.3.619-635.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Butel J. S., Tevethia S. S., Melnick J. L. Oncogenicity and cell transformation by papovavirus SV40: the role of the viral genome. Adv Cancer Res. 1972;15:1–55. doi: 10.1016/s0065-230x(08)60371-1. [DOI] [PubMed] [Google Scholar]
  6. Carroll R. B., Hager L., Dulbecco R. Simian virus 40 T antigen binds to DNA. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3754–3757. doi: 10.1073/pnas.71.9.3754. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Carroll R. B., Smith A. E. Monomer molecular weight of T antigen from simian virus 40-infected and transformed cells. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2254–2258. doi: 10.1073/pnas.73.7.2254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Chang C., Anderson J. L., Martin R. G., Mora P. T. Expression of tumor-specific transplantation antigen in cell lines transformed by wild-type of tsA mutant simian virus 40. J Virol. 1977 May;22(2):281–289. doi: 10.1128/jvi.22.2.281-289.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Chang C., Luborsky S. W., Mora P. T. Tumour-specific transplantation antigen from SV40 transformed cells binds to DNA. Nature. 1977 Sep 29;269(5627):438–440. doi: 10.1038/269438a0. [DOI] [PubMed] [Google Scholar]
  10. Chang C., Pancake S. J., Luborsky S. W., Mora P. T. Detergent solubilization and partial purification of tumor specific surface and transplantation antigens from SV40-virus-transformed mouse cells. Int J Cancer. 1977 Feb 15;19(2):258–266. doi: 10.1002/ijc.2910190216. [DOI] [PubMed] [Google Scholar]
  11. Crawford L. V., Cole C. N., Smith A. E., Paucha E., Tegtmeyer P., Rundell K., Berg P. Organization and expression of early genes of simian virus 40. Proc Natl Acad Sci U S A. 1978 Jan;75(1):117–121. doi: 10.1073/pnas.75.1.117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Cullen S. E., Schwartz B. D. An improved method for isolation of H-2 and Ia alloantigens with immunoprecipitation induced by protein A-bearing staphylococci. J Immunol. 1976 Jul;117(1):136–142. [PubMed] [Google Scholar]
  13. DEFENDI V. Effect of SV 40 virus immunization on growth of transplantable SV 40 and polyoma virus tumors in hamsters. Proc Soc Exp Biol Med. 1963 May;113:12–16. doi: 10.3181/00379727-113-28261. [DOI] [PubMed] [Google Scholar]
  14. Dorval G., Welsh K. I., Wigzell H. A radioimmunoassay of cellular surface antigens on living cells using iodinated soluble protein A from Staphylococcus aureus. J Immunol Methods. 1975 Jun;7(2-3):237–250. doi: 10.1016/0022-1759(75)90021-6. [DOI] [PubMed] [Google Scholar]
  15. Drapkin M. S., Appella E., Law L. W. Immunogenic properties of a soluble tumor-specific transplantation antigen induced by Simian virus 40. J Natl Cancer Inst. 1974 Jan;52(1):259–264. doi: 10.1093/jnci/52.1.259. [DOI] [PubMed] [Google Scholar]
  16. Greenblatt J. F., Allet B., Weil R., Ahmad-Zadeh C. Synthesis of the tumour antigen and the major capsid protein of simian virus 40 in a cell-free system derived from Escherichia coli. J Mol Biol. 1976 Dec;108(2):361–379. doi: 10.1016/s0022-2836(76)80125-8. [DOI] [PubMed] [Google Scholar]
  17. HUEBNER R. J., ROWE W. P., TURNER H. C., LANE W. T. SPECIFIC ADENOVIRUS COMPLEMENT-FIXING ANTIGENS IN VIRUS-FREE HAMSTER AND RAT TUMORS. Proc Natl Acad Sci U S A. 1963 Aug;50:379–389. doi: 10.1073/pnas.50.2.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Henderson I. C., Livingston D. M. Partial purification and characterization of the SV40 T antigen. Cell. 1974 Sep;3(1):65–70. doi: 10.1016/0092-8674(74)90041-5. [DOI] [PubMed] [Google Scholar]
  19. Ito Y., Brocklehurst J. R., Dulbecco R. Virus-specific proteins in the plasma membrane of cells lytically infected or transformed by pol-oma virus. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4666–4670. doi: 10.1073/pnas.74.10.4666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. KHERA K. S., ASHKENAZI A., RAPP F., MELNICK J. L. IMMUNITY IN HAMSTERS TO CELLS TRANSFORMED IN VITRO AND IN VIVO BY SV40. TESTS FOR ANTIGENIC RELATIONSHIP AMONG THE PAPOVAVIRUSES. J Immunol. 1963 Nov;91:604–613. [PubMed] [Google Scholar]
  21. KOCH M. A., SABIN A. B. Specificity of virus-induced resistance to transplantation of polyoma and SV 40 tumors in adult hamsters. Proc Soc Exp Biol Med. 1963 May;113:4–12. doi: 10.3181/00379727-113-28260. [DOI] [PubMed] [Google Scholar]
  22. Kit S., Kurimura T., Dubbs D. R. Transplantable mouse tumor line induced by injection of SV40-transformed mouse kidney cells. Int J Cancer. 1969 Jul 15;4(4):384–392. doi: 10.1002/ijc.2910040403. [DOI] [PubMed] [Google Scholar]
  23. Kuchino T., Yamaguchi N. Characterization of T antigen in cells infected with a temperature-sensitive mutant of simian virus 40. J Virol. 1975 Jun;15(6):1302–1307. doi: 10.1128/jvi.15.6.1302-1307.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  25. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  26. Lewis A. M., Jr, Rowe W. P. Studies of nondefective Adenovirus 2-Simian virus 40 hybrid viruses. 8. Association of Simian virus 40 transplantation antigen with a specific region of the early viral genome. J Virol. 1973 Oct;12(4):836–840. doi: 10.1128/jvi.12.4.836-840.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Martin R. G., Chou J. Y. Simian virus 40 functions required for the establishment and maintenance of malignant transformation. J Virol. 1975 Mar;15(3):599–612. doi: 10.1128/jvi.15.3.599-612.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. McCollester D. L. Isolation of meth A cell surface membranes possessing tumor-specific transplantation antigen activity. Cancer Res. 1970 Dec;30(12):2832–2837. [PubMed] [Google Scholar]
  29. Osborn M., Weber K. Simian virus 40 gene A function and maintenance of transformation. J Virol. 1975 Mar;15(3):636–644. doi: 10.1128/jvi.15.3.636-644.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. POPE J. H., ROWE W. P. DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE. J Exp Med. 1964 Aug 1;120:121–128. doi: 10.1084/jem.120.2.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Prives C., Gilboa E., Revel M., Winocour E. Cell-free translation of simian virus 40 early messenger RNA coding for viral T-antigen. Proc Natl Acad Sci U S A. 1977 Feb;74(2):457–461. doi: 10.1073/pnas.74.2.457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. RAPP F., BUTEL J. S., MELNICK J. L. VIRUS-INDUCED INTRANUCLEAR ANTIGEN IN CELLS TRANSFORMED BY PAPOVAVIRUS SV40. Proc Soc Exp Biol Med. 1964 Aug-Sep;116:1131–1135. doi: 10.3181/00379727-116-29472. [DOI] [PubMed] [Google Scholar]
  33. Rogers M. J., Law L. W., Appella E. Subcellular distribution of the tumor-specific transplantation antigen of simian virus 40-transformed cells. J Natl Cancer Inst. 1977 Oct;59(4):1291–1295. doi: 10.1093/jnci/59.4.1291. [DOI] [PubMed] [Google Scholar]
  34. Rundell K., Collins J. K., Tegtmeyer P., Ozer H. L., Lai C. J., Nathans D. Identification of simian virus 40 protein A. J Virol. 1977 Feb;21(2):636–646. doi: 10.1128/jvi.21.2.636-646.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Smith R. W., Morganroth J., Mora P. T. SV40 virus-induced tumour specific transplantation antigen in cultured mouse cells. Nature. 1970 Jul 11;227(5254):141–145. doi: 10.1038/227141a0. [DOI] [PubMed] [Google Scholar]
  36. Tegtmeyer P. Function of simian virus 40 gene A in transforming infection. J Virol. 1975 Mar;15(3):613–618. doi: 10.1128/jvi.15.3.613-618.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Tenen D. G., Baygell P., Livingston D. M. Thermolabile T (tumor) antigen from cells transformed by a temperature-sensitive mutant of simian virus 40. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4351–4355. doi: 10.1073/pnas.72.11.4351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Tenen D. G., Garewal H., Haines L. L., Hudson J., Woodard V., Light S., Livingston D. M. Purification of simian virus 40 tumor antigen from a line of simian virus 40-transformed human cells. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3745–3749. doi: 10.1073/pnas.74.9.3745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Tevethia M. J., Tevethia S. S. Biology of simian virus 40 (SV40) transplantation antigen (TrAg) III. Involvement of SV40 gene A in the expression of TrAg in permissive cells. Virology. 1977 Sep;81(2):212–223. doi: 10.1016/0042-6822(77)90138-6. [DOI] [PubMed] [Google Scholar]
  40. Tevethia S. S., Couvillionla, Rapp F. Development in hamsters of antibodies against surface antigens present in cells transformed by papovavirus SV40. J Immunol. 1968 Feb;100(2):358–362. [PubMed] [Google Scholar]
  41. Tjian R., Fey G., Graessmann A. Biological activity of purified simian virus 40 T antigen proteins. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1279–1283. doi: 10.1073/pnas.75.3.1279. [DOI] [PMC free article] [PubMed] [Google Scholar]

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