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. 1979 Jan;29(1):220–231. doi: 10.1128/jvi.29.1.220-231.1979

Terminally redundant deletion mutants of bacteriophage BF23.

A R Shaw, D Lang, D J McCorquodale
PMCID: PMC353104  PMID: 430593

Abstract

Deletion mutants of bacteriophage BF23 were isolated and the positions of the deletions were determined. Two different deletable regions were detected: one in the same region as previously reported for bacteriophage T5, which is closely related to BF23; and the other within both terminal repetitions. The former deletable region lay between positions 0.31 and 0.36, which represented the fractional lengths of the BF23 ( + ) DNA as measured from its left end. The latter deletion was evenly divided between the two terminal repetitions. The deletion in the left terminal repetition lay between positions 0.044 and 0.078 and was repeated in the corresponding region of the right terminal repetition between positions 0.966 and 1.0. The size of the DNA transferred to host cells during the first step of DNA transfer by BF23 carrying deletions in the terminal repetitions of its DNA was less than the size of DNA transferred during the first step by wild-type BF23 by an amount equal to the size of the deletion in each terminal repetition. This finding suggests the existence of a specific mechanism for delineating the position at which the first step of DNA transfer is stopped.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADAMS M. H., LARK G. Mutation to heat resistance in coliphage T51. J Immunol. 1950 Apr;64(4):335–347. [PubMed] [Google Scholar]
  2. ADAMS M. H. The genotypically and phenotypically heat resistant forms in the T5 species of bacteriophage. Ann Inst Pasteur (Paris) 1953 Jan;84(1):164–174. [PubMed] [Google Scholar]
  3. BURGI E., HERSHEY A. D. Specificity and concentration limit in self-protection against mechanical breakage of DNA. J Mol Biol. 1962 Apr;4:313–315. doi: 10.1016/s0022-2836(62)80009-6. [DOI] [PubMed] [Google Scholar]
  4. Bujard H. Location of single-strand interruptions in the DNA of bacteriophage T5. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1167–1174. doi: 10.1073/pnas.62.4.1167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chen C. Transcription map of bacteriophage T5. Virology. 1976 Oct 1;74(1):116–127. doi: 10.1016/0042-6822(76)90134-3. [DOI] [PubMed] [Google Scholar]
  6. HERTEL R., MARCHI L., MULLER K. Density mutants of phage T5. Virology. 1962 Dec;18:576–581. doi: 10.1016/0042-6822(62)90060-0. [DOI] [PubMed] [Google Scholar]
  7. Hamlett N. V., Lange-Gufstafson B., Rhoades M. Physical map of the bacteriophage T5 genome based on the cleavage products of the restriction endonucleases SalI, SmaI, BamI, and HpaI. J Virol. 1977 Oct;24(1):249–260. doi: 10.1128/jvi.24.1.249-260.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hayward G. S., Smith M. G. The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis. J Mol Biol. 1972 Feb 14;63(3):383–395. doi: 10.1016/0022-2836(72)90435-4. [DOI] [PubMed] [Google Scholar]
  9. Jacquemin-Sablon A., Richardson C. C. Analysis of the interruptions in bacteriophage T5 DNA. J Mol Biol. 1970 Feb 14;47(3):477–493. doi: 10.1016/0022-2836(70)90316-5. [DOI] [PubMed] [Google Scholar]
  10. LANG D., Shaw A. R., McCorquodale D. J. Molecular weights of DNA from bacteriophages T5, T5st(O), BF23, and BF23st(4). J Virol. 1975 Jan;17(1):296–297. doi: 10.1128/jvi.17.1.296-297.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lang D., Mitani M. Simplified quantitative electron microscopy of biopolymers. Biopolymers. 1970;9(3):373–379. doi: 10.1002/bip.1970.360090310. [DOI] [PubMed] [Google Scholar]
  12. McCorquodale D. J., Shaw A. R., Shaw P. K., Chinnadurai G. Pre-early polypeptides of bacteriophages T5 and BF23. J Virol. 1977 May;22(2):480–488. doi: 10.1128/jvi.22.2.480-488.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McCorquodale D. J. The T-odd bacteriophages. CRC Crit Rev Microbiol. 1975 Dec;4(2):101–159. doi: 10.3109/10408417509111574. [DOI] [PubMed] [Google Scholar]
  14. Mizobuchi K., Anderson G. C., McCorquodale D. J. Abortive infection by bacteriophage BF23 due to the colicin Ib factor. I. Genetic studies of nonrestricted and amber mutants of bacteriophage BF23. Genetics. 1971 Jul;68(3):323–340. doi: 10.1093/genetics/68.3.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Neidhardt F. C., Bloch P. L., Smith D. F. Culture medium for enterobacteria. J Bacteriol. 1974 Sep;119(3):736–747. doi: 10.1128/jb.119.3.736-747.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rhoades M. Cleavage of T5 DNA by the Escherichia coli R-I restriction endonuclease. Virology. 1975 Mar;64(1):170–179. doi: 10.1016/0042-6822(75)90089-6. [DOI] [PubMed] [Google Scholar]
  17. Rhoades M. Localization of single-chain interruptions in bacteriophage T5 DNA. II. Electrophoretic studies. J Virol. 1977 Sep;23(3):737–750. doi: 10.1128/jvi.23.3.737-750.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rhoades M., Rhoades E. A. Terminal repetition in the DNA of bacteriophage T5. J Mol Biol. 1972 Aug 21;69(2):187–200. doi: 10.1016/0022-2836(72)90224-0. [DOI] [PubMed] [Google Scholar]
  19. Saigo K. A new bacteriophage T5 particle with a DNA lacking the terminal repetition at the "right-hand" end. J Mol Biol. 1976 Nov 5;107(3):369–378. doi: 10.1016/s0022-2836(76)80010-1. [DOI] [PubMed] [Google Scholar]
  20. Saigo K. Denaturation mapping and chromosome structure in bacteriophage T5. Virology. 1975 Nov;68(1):166–172. doi: 10.1016/0042-6822(75)90158-0. [DOI] [PubMed] [Google Scholar]
  21. Saigo K. Tail--DNA connection and chromosome structure in bacteriophage T5. Virology. 1975 Nov;68(1):154–165. doi: 10.1016/0042-6822(75)90157-9. [DOI] [PubMed] [Google Scholar]
  22. Scheible P. P., Rhoades E. A., Rhoades M. Localization of single-chain interruptions in bacteriophage T5 DNA I. Electron microscopic studies. J Virol. 1977 Sep;23(3):725–736. doi: 10.1128/jvi.23.3.725-736.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Scheible P. P., Rhoades M. Heteroduplex mapping of heat-resistant deletion mutants of bacteriophage t5. J Virol. 1975 May;15(5):1276–1280. doi: 10.1128/jvi.15.5.1276-1280.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. von Gabain A., Hayward G. S., Bujard H. Physical mapping of the HindIII, EcoRI, Sal and Sma restriction endonuclease cleavage fragments from bacteriophage T5 DNA. Mol Gen Genet. 1976 Feb 2;143(3):279–290. doi: 10.1007/BF00269404. [DOI] [PubMed] [Google Scholar]

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