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. 1979 Apr;30(1):375–383. doi: 10.1128/jvi.30.1.375-383.1979

Structure of products of the Moloney murine leukemia virus endogenous DNA polymerase reaction.

W A Haseltine, J M Coffin, T C Hageman
PMCID: PMC353331  PMID: 90161

Abstract

We have investigated the process by which the single-stranded RNA genome of Moloney murine leukemia virus is copied into DNA in vitro. DNA synthesis if initiated near the 5' end of the genome, and the elongation of the growing chain occurs by a jumping mechanism whereby the DNA synthesized at the 5' end of the genome is elongated along the 3' end. Unique DNA fragments synthesized beyond the 5' end of the genome in vitro have, at their 5' and 3' ends, copies of unique sequences from the 5' and 3' ends of the genome. These flank a copy of the 49- to 60-nucleotide terminally redundant sequence. These results indicate that the terminal redundancy serves as a "bridge" to allow a DNA molecule synthesized at the 5' end of the genome to serve as a primer for synthesis from the 3' end.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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