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. 1979 Aug;31(2):288–298. doi: 10.1128/jvi.31.2.288-298.1979

Functional relationship between bacteriophages G4 and phi X174.

W E Borrias, M Hagenaar, R Van Den Brekel, C Kühlemeijer, P J Weisbeek
PMCID: PMC353451  PMID: 480475

Abstract

Mutants of bacteriophage G4 were isolated and characterized, and their mutations were mapped. They constitute six different genes, namely, A, B, E, F, G, and H. The functional relationship with bacteriophage phi X174 was determined by complementation experiments using amber mutants of phi X and amber mutants of G4. Bacteriophage phi X was able to use the products of G4 genes E, F, G, and H. In bacteriophage G4, however, only the phi X gene H product was functional.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barrell B. G., Air G. M., Hutchison C. A., 3rd Overlapping genes in bacteriophage phiX174. Nature. 1976 Nov 4;264(5581):34–41. doi: 10.1038/264034a0. [DOI] [PubMed] [Google Scholar]
  2. Benbow R. M., Hutchison C. A., Fabricant J. D., Sinsheimer R. L. Genetic Map of Bacteriophage phiX174. J Virol. 1971 May;7(5):549–558. doi: 10.1128/jvi.7.5.549-558.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benbow R. M., Mayol R. F., Picchi J. C., Sinsheimer R. L. Direction of Translation and Size of Bacteriophage phiX174 Cistrons. J Virol. 1972 Jul;10(1):99–114. doi: 10.1128/jvi.10.1.99-114.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Benbow R. M., Zuccarelli A. J., Davis G. C., Sinsheimer R. L. Genetic recombination in bacteriophage phi chi 174. J Virol. 1974 Apr;13(4):898–907. doi: 10.1128/jvi.13.4.898-907.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  6. Borrias W. E., Van de Pol J. H. Genetic complementation between bacteriophage oX174 DNA molecules in spheroplasts. Mol Gen Genet. 1972;114(1):59–67. doi: 10.1007/BF00268747. [DOI] [PubMed] [Google Scholar]
  7. Borrias W. E., Weisbeek P. J., Van Arkel G. A. An intracistronic region of gene A of bacteriophage piX174 not involved in progeny RF DNA synthesis. Nature. 1976 May 20;261(5557):245–248. doi: 10.1038/261245a0. [DOI] [PubMed] [Google Scholar]
  8. Borrias W. E., Wilschut I. J., Vereijken J. M., Weisbeek P. J., van Arkel G. A. Induction and isolation of mutants in a specific region of gene A of bacteriophage phi chi 174. Virology. 1976 Mar;70(1):195–197. doi: 10.1016/0042-6822(76)90251-8. [DOI] [PubMed] [Google Scholar]
  9. Borrias W. E., van de Pol J. H., van de Vate C., van Arkel G. A. Complementation experiments between conditional lethal mutants of bacteriophage psiX 174. Mol Gen Genet. 1969 Oct 13;105(2):152–163. doi: 10.1007/BF00445685. [DOI] [PubMed] [Google Scholar]
  10. Burgess A. B., Denhardt D. T. Studies on phiX174 proteins. I. Phage-specific proteins synthesized after infection of Escherichia coli. J Mol Biol. 1969 Sep 28;44(3):377–386. doi: 10.1016/0022-2836(69)90367-2. [DOI] [PubMed] [Google Scholar]
  11. Compton J. L., Singheimer R. L. Aligning the phiX174 genetic map and the phiX174/S13 heterduplex denaturation map. J Mol Biol. 1977 Jan 15;109(2):217–234. doi: 10.1016/s0022-2836(77)80031-4. [DOI] [PubMed] [Google Scholar]
  12. Derstine P. L., Dumas L. B., Miller C. A. Bacteriophage G4 DNA synthesis in temperature-sensitive dna mutants of Escherichia coli. J Virol. 1976 Sep;19(3):915–924. doi: 10.1128/jvi.19.3.915-924.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. GUTHRIE G. D., SINSHEIMER R. L. Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage phi X174. Biochim Biophys Acta. 1963 Jun 25;72:290–297. [PubMed] [Google Scholar]
  14. Gelfand D. H., Hayashi M. Electrophoretic characterization of phiX174-specific proteins. J Mol Biol. 1969 Sep 28;44(3):501–516. doi: 10.1016/0022-2836(69)90376-3. [DOI] [PubMed] [Google Scholar]
  15. Godson G. N., Barrell B. G., Staden R., Fiddes J. C. Nucleotide sequence of bacteriophage G4 DNA. Nature. 1978 Nov 16;276(5685):236–247. doi: 10.1038/276236a0. [DOI] [PubMed] [Google Scholar]
  16. Godson G. N. DNA heteroduplex analysis of the relation between bacteriophages phiX174 and S.13. J Mol Biol. 1973 Jul 15;77(4):467–477. doi: 10.1016/0022-2836(73)90216-7. [DOI] [PubMed] [Google Scholar]
  17. Godson G. N. Evolution of phi chi 174. II. A cleavage map of the G4 phage genome and comparison with the cleavage map of phi chi 174. Virology. 1975 Feb;63(2):320–325. doi: 10.1016/0042-6822(75)90306-2. [DOI] [PubMed] [Google Scholar]
  18. Godson G. N. Evolution of phi-chi 174. Isolation of four new phi-chi-like phages and comparison with phi-chi 174. Virology. 1974 Mar;58(1):272–289. doi: 10.1016/0042-6822(74)90161-5. [DOI] [PubMed] [Google Scholar]
  19. Hayashi M. N., Hayashi M. Fragment maps of phiX-174 replicative DNA produced by restriction enzymes from haemophilus aphirophilus and haemophilus influenzae H-I. J Virol. 1974 Nov;14(5):1142–1151. doi: 10.1128/jvi.14.5.1142-1151.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Holloman W. K., Radding C. M. Recombination promoted by superhelical DNA and the recA gene of Escherichia coli. Proc Natl Acad Sci U S A. 1976 Nov;73(11):3910–3914. doi: 10.1073/pnas.73.11.3910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Hutchison C. A., 3rd, Sinsheimer R. L. The process of infection with bacteriophage phi-X174. X. Mutations in a phi-X Lysis gene. J Mol Biol. 1966 Jul;18(3):429–447. doi: 10.1016/s0022-2836(66)80035-9. [DOI] [PubMed] [Google Scholar]
  22. Jeng Y., Gelfand D., Hayashi M., Shleser R., Tessman E. S. The eight genes of bacteriophages phi X174 and S13 and comparison of the phage-specified proteins. J Mol Biol. 1970 Apr 28;49(2):521–526. doi: 10.1016/0022-2836(70)90262-7. [DOI] [PubMed] [Google Scholar]
  23. Keegstra W., Godson G. N., Weisbeek P. J., Jansz H. S. Comparison of the G4 and phiX174 phage genomes by electron microscopy. Virology. 1979 Mar;93(2):527–536. doi: 10.1016/0042-6822(79)90255-1. [DOI] [PubMed] [Google Scholar]
  24. Kodaira K. I., Taketo A. Conversion of bacteriophage G4 single-stranded viral DNA to double-stranded replicative form in dna mutants of Escherichia coli. Biochim Biophys Acta. 1977 May 17;476(2):149–155. doi: 10.1016/0005-2787(77)90091-0. [DOI] [PubMed] [Google Scholar]
  25. McMacken R., Kornberg A. A multienzyme system for priming the replication of phiX174 viral DNA. J Biol Chem. 1978 May 10;253(9):3313–3319. [PubMed] [Google Scholar]
  26. Radloff R., Bauer W., Vinograd J. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proc Natl Acad Sci U S A. 1967 May;57(5):1514–1521. doi: 10.1073/pnas.57.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sanger F., Air G. M., Barrell B. G., Brown N. L., Coulson A. R., Fiddes C. A., Hutchison C. A., Slocombe P. M., Smith M. Nucleotide sequence of bacteriophage phi X174 DNA. Nature. 1977 Feb 24;265(5596):687–695. doi: 10.1038/265687a0. [DOI] [PubMed] [Google Scholar]
  28. Schekman R., Weiner A., Kornberg A. Multienzyme systems of DNA replication. Science. 1974 Dec 13;186(4168):987–993. doi: 10.1126/science.186.4168.987. [DOI] [PubMed] [Google Scholar]
  29. Shaw D. C., Walker J. E., Northrop F. D., Barrell B. G., Godson G. N., Fiddes J. C. Gene K, a new overlapping gene in bacteriophage G4. Nature. 1978 Apr 6;272(5653):510–515. doi: 10.1038/272510a0. [DOI] [PubMed] [Google Scholar]
  30. Siden E. J., Hayashi M. Role of the gene beta-product in bacteriophage phi-X174 development. J Mol Biol. 1974 Oct 15;89(1):1–16. doi: 10.1016/0022-2836(74)90159-4. [DOI] [PubMed] [Google Scholar]
  31. TESSMAN E. S. COMPLEMENTATION GROUPS IN PHAGE S13. Virology. 1965 Feb;25:303–321. doi: 10.1016/0042-6822(65)90208-4. [DOI] [PubMed] [Google Scholar]
  32. TESSMAN E. S., SHLESER R. Genetic recombination between phages S13 and phi-X174. Virology. 1963 Feb;19:239–240. doi: 10.1016/0042-6822(63)90015-1. [DOI] [PubMed] [Google Scholar]
  33. Taketo A. Replication of phiA and phiX174 in Escherichia coli mutants thermosensitive in DNA synthesis. Mol Gen Genet. 1975 Sep 8;139(4):285–291. doi: 10.1007/BF00267968. [DOI] [PubMed] [Google Scholar]
  34. Taketo A. Sensitivity of Escherichia coli to viral nucleic acid. VI. Capacity of dna mutants and DNA polymerase-less mutants for multiplication of phiA and phiX 174. Mol Gen Genet. 1973 Mar 27;122(1):15–22. doi: 10.1007/BF00337970. [DOI] [PubMed] [Google Scholar]
  35. Tessman E. S. Mutants of bacteriophage S13 blocked in infectious DNA synthesis. J Mol Biol. 1966 May;17(1):218–236. doi: 10.1016/s0022-2836(66)80104-3. [DOI] [PubMed] [Google Scholar]
  36. Tessman I., Kumar S., Tessman E. S. Direction of translation in bacteriophage S13. Science. 1967 Oct 13;158(3798):267–268. doi: 10.1126/science.158.3798.267. [DOI] [PubMed] [Google Scholar]
  37. Thompson B. J., Sussman C. H., Warner R. C. Coinfection of E. coli with phages G4 and phiX174: origin of dimeric replicative form species. Virology. 1978 Jun 1;87(1):212–215. doi: 10.1016/0042-6822(78)90175-7. [DOI] [PubMed] [Google Scholar]
  38. Vereijken J. M., Van Mansfeld A. D., Baas P. D., Jansz H. S. Arthrobacter luteus restriction endonuclease cleavage map of phi chi 174 RF DNA. Virology. 1975 Nov;68(1):221–233. doi: 10.1016/0042-6822(75)90163-4. [DOI] [PubMed] [Google Scholar]
  39. Weisbeek P. J., Borrias W. E., Langeveld S. A., Baas P. D., Van Arkel G. A. Bacteriophage phiX174: gene A overlaps gene B. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2504–2508. doi: 10.1073/pnas.74.6.2504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Weisbeek P. J., Vereijken J. M., Baas P. D., Jansz H. S., Van Arkel G. A. The genetic map of bacteriophage phiX174 constructed with restriction enzyme fragments. Virology. 1976 Jul 1;72(1):61–71. doi: 10.1016/0042-6822(76)90311-1. [DOI] [PubMed] [Google Scholar]
  41. Wickner S., Hurwitz J. Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4120–4124. doi: 10.1073/pnas.71.10.4120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. ZAHLER S. A. Some biological properties of bacteriophages S13 and theta X-174. J Bacteriol. 1958 Mar;75(3):310–315. doi: 10.1128/jb.75.3.310-315.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Zechel K., Bouché J. P., Kornberg A. Replication of phage G4. A novel and simple system for the initiation of deoxyribonucleic acid synthesis. J Biol Chem. 1975 Jun 25;250(12):4684–4689. [PubMed] [Google Scholar]
  44. Zuccarelli A. L., Benbow R. M., Sinsheimer R. L. Formation of the parental replicative form of bacteriophage phiX174. J Mol Biol. 1976 Sep 15;106(2):375–402. doi: 10.1016/0022-2836(76)90092-9. [DOI] [PubMed] [Google Scholar]

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