Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1979 Sep;31(3):707–717. doi: 10.1128/jvi.31.3.707-717.1979

Recombination between snowhoe hare and La Crosse bunyaviruses.

J R Gentsch, G Robeson, D H Bishop
PMCID: PMC353499  PMID: 513193

Abstract

We have previously reported heterologous genetic recombination resulting from crosses involving temperature-sensitive (ts) mutants of La Crosse (LAC) group II and snowshoe hare (SSH) group I ts mutants (J. Gentsch, L. R. Wynne, J. P. Clewley, R. E. Shope, and D. H. L. Bishop, J. Virol. 24:893-902, 1977). From those crosses two reassortant viruses having the large/medium/small viral RNA segment genotypes of SSH/LAC/SSH and SSH/LAC/LAC were obtained. In this study it has been found that the reciprocal cross (SSH group II x LAC group I ts mutants) has not yielded the expected LAC/SSH/SSH or LAC/SSH/LAC reassortant viruses. The backcross of a SSH/LAC/SSH group II ts mutant with a LAC group I ts mutant has produced a new reassortant virus, LAC/LAC/SSH, whereas the backcross of SSH/LAC/LAC group I ts mutants with SSH group II ts mutants gave another reassortant, SSH/SSH/LAC. Backcross analyses of LAC/LAC/SSH group I ts mutants with Group II ts mutants of SSH have not yielded the expected LAC/SSH/SSH reassortant virus, nor have backcrosses of SSH/SSH/LAC group II ts mutants with group I ts mutants of LAC virus yielded the expected LAC/SSH/LAC reassortant. Possible reasons why certain reassortant viruses are not produced are discussed. A procedure to screen SSH-LAC reassortant viruses which differ in their virion N polypeptides is described.

Full text

PDF
717

Images in this article

710Image
on p.710
711Image
on p.711
713Image
on p.713
714Image
on p.714

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARDOS V., DANIELOVA V. The Tahyna virus--a virus isolated from mosquitoes in Czechoslovakia. J Hyg Epidemiol Microbiol Immunol. 1959;3:264–276. [PubMed] [Google Scholar]
  2. BURGDORFER W., NEWHOUSE V. F., THOMAS L. A. Isolation of California encephalitis virus from the blood of a snowshoe hare (Lepus americanus) in western Montana. Am J Hyg. 1961 May;73:344–349. doi: 10.1093/oxfordjournals.aje.a120193. [DOI] [PubMed] [Google Scholar]
  3. Bond J. O., Hammon W. M., Lewis A. L., Sather G. E., Taylor D. J. California group arboviruses in Florida and report of a new strain, Keystone virus. Public Health Rep. 1966 Jul;81(7):607–613. [PMC free article] [PubMed] [Google Scholar]
  4. Bouloy M., Krams-Ozden S., Horodniceanu F., Hannoun C. Three-segment RNA genome of Lumbo virus (Bunyavirus). Intervirology. 1973;2(3):173–180. doi: 10.1159/000149420. [DOI] [PubMed] [Google Scholar]
  5. Brummer-Korvenkontio M., Saikku P., Korhonen P., Ulmanen I., Reunala T., Karvonen J. Arboviruses in Finland. IV. Isolation and characterization of Inkoo virus, a Finnish representative of the California group. Am J Trop Med Hyg. 1973 May;22(3):404–413. [PubMed] [Google Scholar]
  6. Clewley J., Gentsch J., Bishop D. H. Three unique viral RNA species of snowshoe hare and La Crosse bunyaviruses. J Virol. 1977 May;22(2):459–468. doi: 10.1128/jvi.22.2.459-468.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DeFoliart G. R., Anslow R. O., Hanson R. P., Morris C. D., Papadopoulos O., Sather G. E. Isolation of Jamestown Canyon serotype of California encephalitis virus from naturally infected Aedes mosquitoes and tabanids. Am J Trop Med Hyg. 1969 May;18(3):440–447. doi: 10.4269/ajtmh.1969.18.440. [DOI] [PubMed] [Google Scholar]
  8. El Said L. H., Vorndam V., Gentsch J. R., Clewley J. P., Calisher C. H., Klimas R. A., Thompson W. H., Grayson M., Trent D. W., Bishop D. H. A comparison of La Crosse virus isolated obtained from different ecological niches and an analysis of the structural components of California encephalitis serogroup viruses and other bunyaviruses. Am J Trop Med Hyg. 1979 Mar;28(2):364–386. doi: 10.4269/ajtmh.1979.28.364. [DOI] [PubMed] [Google Scholar]
  9. Gentsch J. R., Bishop D. H. Small viral RNA segment of bunyaviruses codes for viral nucleocapsid protein. J Virol. 1978 Oct;28(1):417–419. doi: 10.1128/jvi.28.1.417-419.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gentsch J., Bishop D. H., Obijeski J. F. The virus particle nucleic acids and proteins of four bunyaviruses. J Gen Virol. 1977 Feb;34(2):257–268. doi: 10.1099/0022-1317-34-2-257. [DOI] [PubMed] [Google Scholar]
  11. Gentsch J., Bishop D. H. Recombination and complementation between temperature-sensitive mutants of a Bunyavirus, snowshoe hare virus. J Virol. 1976 Oct;20(1):351–354. doi: 10.1128/jvi.20.1.351-354.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gentsch J., Wynne L. R., Clewley J. P., Shope R. E., Bishop D. H. Formation of recombinants between snowshoe hare and La Crosse bunyaviruses. J Virol. 1977 Dec;24(3):893–902. doi: 10.1128/jvi.24.3.893-902.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HAMMON W. M., REEVES W. C., SATHER G. California encephalitis virus, a newly described agent. II. Isolations and attempts to identify and characterize the agent. J Immunol. 1952 Nov;69(5):493–510. [PubMed] [Google Scholar]
  14. Issel C. J. Isolation of Jamestown Canyon virus (a California group arbovirus) from a white-tailed deer. Am J Trop Med Hyg. 1973 May;22(3):414–417. doi: 10.4269/ajtmh.1973.22.414. [DOI] [PubMed] [Google Scholar]
  15. Jennings W. L., Lewis A. L., Sather G. E., Hammon W. M., Bond J. O. California-encephalitis-group viruses in Florida rabbits. Report of experimental and sentinel studies. Am J Trop Med Hyg. 1968 Sep;17(5):781–787. doi: 10.4269/ajtmh.1968.17.781. [DOI] [PubMed] [Google Scholar]
  16. KOKERNOT R. H., McINTOSH B. M., WORTH C. B., DE MORAIST, WEINBREN M. P. Isolation of viruses from mosquitoes collected at Lumbo, Mozambique. I. Lumbo virus, a new virus isolated from Aedes (Skusea) pembaensis Theobald. Am J Trop Med Hyg. 1962 Sep;11:678–682. doi: 10.4269/ajtmh.1962.11.678. [DOI] [PubMed] [Google Scholar]
  17. Karabatsos N. Supplement to International Catalogue of Arboviruses including certain other viruses of vertebrates. Am J Trop Med Hyg. 1978 Mar;27(2 Pt 2 Suppl):372–440. doi: 10.4269/ajtmh.1978.27.372. [DOI] [PubMed] [Google Scholar]
  18. Lindsey H. S., Calisher C. H., Mathews J. H. Serum dilution neutralization test for California group virus identification and serology. J Clin Microbiol. 1976 Dec;4(6):503–510. doi: 10.1128/jcm.4.6.503-510.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Obijeski J. F., Bishop D. H., Murphy F. A., Palmer E. L. Structural proteins of La Crosse virus. J Virol. 1976 Sep;19(3):985–997. doi: 10.1128/jvi.19.3.985-997.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Obijeski J. F., Bishop D. H., Palmer E. L., Murphy F. A. Segmented genome and nucleocapsid of La Crosse virus. J Virol. 1976 Dec;20(3):664–675. doi: 10.1128/jvi.20.3.664-675.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Obijeski J. F., Marchenko A. T., Bishop D. H., Cann B. W., Murphy F. A. Comparative electrophoretic analysis of the virus proteins of four rhabdoviruses. J Gen Virol. 1974 Jan;22(1):21–33. doi: 10.1099/0022-1317-22-1-21. [DOI] [PubMed] [Google Scholar]
  22. Pettersson R. F., Hewlett M. J., Baltimore D., Coffin J. M. The genome of Uukuniemi virus consists of three unique RNA segments. Cell. 1977 May;11(1):51–63. doi: 10.1016/0092-8674(77)90316-6. [DOI] [PubMed] [Google Scholar]
  23. Porterfield J. S., Casals J., Chumakov M. P., Gaidamovich S. Y., Hannoun C., Holmes I. H., Horzinek M. C., Mussgay M., Oker-Blom N., Russell P. K. Bunyaviruses and Bunyaviridae. Intervirology. 1975;6(1):13–24. doi: 10.1159/000149449. [DOI] [PubMed] [Google Scholar]
  24. Porterfield J. S., Casals J., Chumakov M. P., Gaidamovich S. Y., Hannoun C., Holmes I. H., Horzinek M. C., Mussgay M., Russell P. K. Bunyaviruses and bunyaviridae. Intervirology. 1974;2(4):270–272. doi: 10.1159/000149433. [DOI] [PubMed] [Google Scholar]
  25. SPENCE L., ANDERSON C. R., AITKEN T. H., DOWNS W. G. Melao virus, a new agent isolated from Trinidadian mosquitoes. Am J Trop Med Hyg. 1962 Sep;11:687–690. doi: 10.4269/ajtmh.1962.11.687. [DOI] [PubMed] [Google Scholar]
  26. THOMPSON W. H., KALFAYAN B., ANSLOW R. O. ISOLATION OF CALIFORNIA ENCEPHALITIS GROUP VIRUS FROM A FATAL HUMAN ILLNESS. Am J Epidemiol. 1965 Mar;81:245–253. doi: 10.1093/oxfordjournals.aje.a120512. [DOI] [PubMed] [Google Scholar]
  27. WHITMAN L., SHOPE R. E. The California complex of arthropod-borne viruses and its relationship to the Bunyamwera group through Guaroa virus. Am J Trop Med Hyg. 1962 Sep;11:691–696. doi: 10.4269/ajtmh.1962.11.691. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES