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. 1979 Oct;32(1):259–267. doi: 10.1128/jvi.32.1.259-267.1979

Human cytomegalovirus-induced immediate early antigens: analysis in sodium dodecyl sulfate-polyacrylamide gel electrophoresis after immunoprecipitation.

S Michelson, F Horodniceanu, M Kress, M Tardy-Panit
PMCID: PMC353550  PMID: 232177

Abstract

Immediate early antigen (IEA) induced in human lung fibroblasts by human cytomegalovirus was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis after immunoprecipitation with IEA-positive human sera. Two polypeptides of 76,000 daltons (76K) and 82K were detectable within 90 min after infection. Polypeptides of similar molecular weight were also found in immunoprecipitates of human cytomegalovirus-infected cells nonpermissive for virus replication. IEA is located within the nucleus, although some of the 76K material appears to be located on the outer nuclear membrane. Raising salt concentrations in the extraction buffer increased antigen extraction. The contribution of these IEA polypeptides to IEA nuclear fluorescent staining is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boldogh I., Gönczöl E., Váczi L. Cytomegalovirus-induced membrane antigens in productively infected cells. Acta Microbiol Acad Sci Hung. 1977;24(1):21–28. [PubMed] [Google Scholar]
  2. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  3. Furukawa T., Fioretti A., Plotkin S. Growth characteristics of cytomegalovirus in human fibroblasts with demonstration of protein synthesis early in viral replication. J Virol. 1973 Jun;11(6):991–997. doi: 10.1128/jvi.11.6.991-997.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Geder L. Evidence for early nuclear antigens in cytomegalovirus-infected cells. J Gen Virol. 1976 Aug;32(2):315–319. doi: 10.1099/0022-1317-32-2-315. [DOI] [PubMed] [Google Scholar]
  5. Jacobs J. P., Jones C. M., Baille J. P. Characteristics of a human diploid cell designated MRC-5. Nature. 1970 Jul 11;227(5254):168–170. doi: 10.1038/227168a0. [DOI] [PubMed] [Google Scholar]
  6. Jeor S. C., Albrecht T. B., Funk F. D., Rapp F. Stimulation of cellular DNA synthesis by human cytomegalovirus. J Virol. 1974 Feb;13(2):353–362. doi: 10.1128/jvi.13.2.353-362.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kamata T., Tanaka S., Watanabe Y. Human cytomegalovirus-induced chromatin factors responsible for changes in template activity and structure of infected cell chromatin. Virology. 1978 Oct 15;90(2):197–208. doi: 10.1016/0042-6822(78)90303-3. [DOI] [PubMed] [Google Scholar]
  8. Kierszenbaum A. L., Huang E. S. Chromatin pattern consisting of repeating bipartite structures in WI-38 cells infected with human cytomegalovirus. J Virol. 1978 Nov;28(2):661–664. doi: 10.1128/jvi.28.2.661-664.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Michelson-Fiske S., Arnoult J., Febvre H. Growth of cytomegalovirus at supra-optimal temperatures. J Gen Virol. 1977 Sep;36(3):437–447. doi: 10.1099/0022-1317-36-3-437. [DOI] [PubMed] [Google Scholar]
  11. Michelson-Fiske S., Horodniceanu F., Guillon J. C. Immediate early antigens in human cytomegalovirus infected cells. Nature. 1977 Dec 15;270(5638):615–617. doi: 10.1038/270615a0. [DOI] [PubMed] [Google Scholar]
  12. Michelson S., Cabau N., Boue A., Horodniceanu F. Comparison of occurrence of antibodies to human cytomegalovirus as demonstrated by immunofluorescence and indirect hemagglutination techniques. J Clin Microbiol. 1979 Jan;9(1):149–151. doi: 10.1128/jcm.9.1.149-151.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Reynolds D. W. Development of early nuclear antigen in cytomegalovirus infected cells in the presence of RNA and protein synthesis inhibitors. J Gen Virol. 1978 Aug;40(2):475–480. doi: 10.1099/0022-1317-40-2-475. [DOI] [PubMed] [Google Scholar]
  14. Stinski M. F. Sequence of protein synthesis in cells infected by human cytomegalovirus: early and late virus-induced polypeptides. J Virol. 1978 Jun;26(3):686–701. doi: 10.1128/jvi.26.3.686-701.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stinski M. F. Synthesis of proteins and glycoproteins in cells infected with human cytomegalovirus. J Virol. 1977 Sep;23(3):751–767. doi: 10.1128/jvi.23.3.751-767.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tanaka S., Furukawa T., Plotkin S. A. Human cytomegalovirus stimulates host cell RNA synthesis. J Virol. 1975 Feb;15(2):297–304. doi: 10.1128/jvi.15.2.297-304.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tegtmeyer P., Rundell K., Collins J. K. Modification of simian virus 40 protein A. J Virol. 1977 Feb;21(2):647–657. doi: 10.1128/jvi.21.2.647-657.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. The T. H., Klein G., Langenhuysen M. M. Antibody reactions to virus-specific early antigens (EA) in patients with cytomegalovirus (CMV) infection. Clin Exp Immunol. 1974 Jan;16(1):1-7,9-12. [PMC free article] [PubMed] [Google Scholar]
  19. Váczi L., Gönczöl E. The effect of cytosine-arabinoside on the multiplication of cytomegalovirus and on the formation of virus-induced intracellular antigens. Acta Virol. 1973 May;17(3):189–195. [PubMed] [Google Scholar]

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