Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1980 Nov;36(2):480–487. doi: 10.1128/jvi.36.2.480-487.1980

Arrangement of the genome of the human papovavirus RF virus.

A Pater, M M Pater, G di Mayorca
PMCID: PMC353665  PMID: 6253672

Abstract

DNA from plaque-purified RF virus, a variant of BK virus, was found to contain two species of molecules. Hybridization of each DNA species to the fragments of BK virus DNA revealed that one species had a deletion corresponding to at least 50% of the late region and the other had a deletion corresponding to at least 40% of the early region of BK virus DNA. Analysis by cleavage of each RF virus DNA species with restriction endonucleases EcoRI, HindIII, AvaII, and PvuII, when compared with BK virus DNA, revealed that the size and number of fragments were different. These results suggest the loss of some restriction sites and the appearance of new sites, probably as a result of base changes in each RF virus DNA species. Furthermore, analysis of the restriction map of each DNA molecule revealed in insertion(s) in both DNA species.

Full text

PDF
484

Images in this article

481Image
on p.481
482Image
on p.482
482Image
on p.482
484Image
on p.484
487Image
on p.487

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brockman W. W. Evolutionary variants of simian virus 40. Prog Med Virol. 1977;23:69–95. [PubMed] [Google Scholar]
  2. Danna K. J., Sack G. H., Jr, Nathans D. Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome. J Mol Biol. 1973 Aug 5;78(2):363–376. doi: 10.1016/0022-2836(73)90122-8. [DOI] [PubMed] [Google Scholar]
  3. Dougherty R. M., DiStefano H. S. Isolation and characterization of a papovavirus from human urine. Proc Soc Exp Biol Med. 1974 Jun;146(2):481–487. doi: 10.3181/00379727-146-38131. [DOI] [PubMed] [Google Scholar]
  4. Dougherty R. M. Induction of tumors in Syrian hamsters by a human renal papovavirus, RF strain. J Natl Cancer Inst. 1976 Aug;57(2):395–400. doi: 10.1093/jnci/57.2.395. [DOI] [PubMed] [Google Scholar]
  5. Gardner S. D., Field A. M., Coleman D. V., Hulme B. New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet. 1971 Jun 19;1(7712):1253–1257. doi: 10.1016/s0140-6736(71)91776-4. [DOI] [PubMed] [Google Scholar]
  6. Howley P. M., Khoury G., Byrne J. C., Takemoto K. K., Martin M. A. Physical map of the BK virus genome. J Virol. 1975 Oct;16(4):959–973. doi: 10.1128/jvi.16.4.959-973.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Huang A. S. Defective interfering viruses. Annu Rev Microbiol. 1973;27:101–117. doi: 10.1146/annurev.mi.27.100173.000533. [DOI] [PubMed] [Google Scholar]
  8. Lecatsas G., Prozesky O. W. Excretion of morphological variants of human polyoma virus. Arch Virol. 1975;47(4):393–397. doi: 10.1007/BF01347981. [DOI] [PubMed] [Google Scholar]
  9. Lecatsas G., Prozesky O. W., van Wyk J., Els H. J. Papova virus in urine after renal transplantation. Nature. 1973 Feb 2;241(5388):343–344. doi: 10.1038/241343a0. [DOI] [PubMed] [Google Scholar]
  10. Maniatis T., Kee S. G., Efstratiadis A., Kafatos F. C. Amplification and characterization of a beta-globin gene synthesized in vitro. Cell. 1976 Jun;8(2):163–182. doi: 10.1016/0092-8674(76)90001-5. [DOI] [PubMed] [Google Scholar]
  11. Miao R., Dougherty R. M. Characterization of human papovavirus RFV: comparison with SV40 and BKV. J Gen Virol. 1977 Apr;35(1):67–75. doi: 10.1099/0022-1317-35-1-67. [DOI] [PubMed] [Google Scholar]
  12. Pater M. M., Pater A., di Mayorca G. Comparative analysis of GS and BK virus genomes. J Virol. 1979 Oct;32(1):220–225. doi: 10.1128/jvi.32.1.220-225.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  14. Scott W. A., Brockman W. W., Nathans D. Biological activities of deletion mutants of simian virus 40. Virology. 1976 Dec;75(2):319–334. doi: 10.1016/0042-6822(76)90031-3. [DOI] [PubMed] [Google Scholar]
  15. Seif I., Khoury G., Dhar R. The genome of human papovavirus BKV. Cell. 1979 Dec;18(4):963–977. doi: 10.1016/0092-8674(79)90209-5. [DOI] [PubMed] [Google Scholar]
  16. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  17. Takemoto K. K., Rabson A. S., Mullarkey M. F., Blaese R. M., Garon C. F., Nelson D. Isolation of papovavirus from brain tumor and urine of a patient with Wiskott-Aldrich syndrome. J Natl Cancer Inst. 1974 Nov;53(5):1205–1207. doi: 10.1093/jnci/53.5.1205. [DOI] [PubMed] [Google Scholar]
  18. Upcroft P., Skolnik H., Upcroft J. A., Solomon D., Khoury G., Hamer D. H., Fareed G. C. Transduction of a bacterial gene into mammalian cells. Proc Natl Acad Sci U S A. 1978 May;75(5):2117–2121. doi: 10.1073/pnas.75.5.2117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wright P. J., Bernhardt G., Major E. O., di Mayorca G. Comparison of the serology, transforming ability, and polypeptide composition of human papovaviruses isolated from urine. J Virol. 1976 Mar;17(3):762–775. doi: 10.1128/jvi.17.3.762-775.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yang R. C., Van de Voorde A., Fiers W. Cleavage map of the simian-virus-40 genome by the restriction endonuclease III of Haemopholus aegyptius. Eur J Biochem. 1976 Jan 2;61(1):101–117. doi: 10.1111/j.1432-1033.1976.tb10002.x. [DOI] [PubMed] [Google Scholar]
  22. Yang R. C., Wu R. BK virus DNA: complete nucleotide sequence of a human tumor virus. Science. 1979 Oct 26;206(4417):456–462. doi: 10.1126/science.228391. [DOI] [PubMed] [Google Scholar]
  23. Yang R. C., Wu R. Cleavage map of BK virus DNA with restriction endonucleases MboI and HaeIII. J Virol. 1978 Sep;27(3):700–712. doi: 10.1128/jvi.27.3.700-712.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Yang R. C., Wu R. Comparative study of papovavirus DNA: BKV(MM), BKV(WT) and SV40. Nucleic Acids Res. 1979 Oct 10;7(3):651–668. doi: 10.1093/nar/7.3.651. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES