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. 1980 Dec;36(3):787–795. doi: 10.1128/jvi.36.3.787-795.1980

Structural relationships between the surface antigens of ground squirrel hepatitis virus and human hepatitis B virus.

W H Gerlich, M A Feitelson, P L Marion, W S Robinson
PMCID: PMC353706  PMID: 7463556

Abstract

Several physical, chemical, and serological properties of surface antigen particles from ground squirrel hepatitis virus (GSHsAg) and human hepatitis B virus (HBsAg) were compared. GSHsAg and HBsAg particles were purified from positive sera by gel chromatography and isopycnic centrifugation. Both antigens consisted mainly of spherical particles with an average diameter of approximately 20 nm and a buoyant density in CsCl of approximately 1.19 g/ml. Their UV absorption spectra indicated the presence of more tryptophane than tyrosine and the absence of detectable nucleic acid. GSHsAg was found to contain two major polypeptides of approximately 23,000 and 27,000 daltons, with electrophoretic migration rates distinctly faster than those of the two major polypeptides of HBsAg particles. After radiolabeling of purified antigen preparations with Bolton-Hunter reagent, the two major polypeptides of GSHsAg showed almost identical tryptic peptide maps. The tryptic peptide map of the major polypeptide from GSHsAg contained 13 of 37 spots also present in the map of the major HBsAg polypeptide, and 13 of 27 spots in the map of the major HBsAg polypeptide were also present in the map of the major GSHsAg polypeptide. This suggests considerable sequence homology between the major surface antigen polypeptides of the two viruses. However, there was only a weak serological cross-reactivity between antigens of the two viruses. Using an anti-HBs-containing serum with a relatively strong cross-reactivity, GSHsAg was found to consist of at least two antigenically different subspecies. The more strongly cross-reacting from had a slightly higher buoyant density than the other antigenic form.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bancroft W. H., Mundon F. K., Russell P. K. Detection of additional antigenic determinants of hepatitis B antigen. J Immunol. 1972 Oct;109(4):842–848. [PubMed] [Google Scholar]
  2. Bayer M. E., Blumberg B. S., Werner B. Particles associated with Australia antigen in the sera of patients with leukaemia, Down's Syndrome and hepatitis. Nature. 1968 Jun 15;218(5146):1057–1059. doi: 10.1038/2181057a0. [DOI] [PubMed] [Google Scholar]
  3. Bolton A. E., Hunter W. M. The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Biochem J. 1973 Jul;133(3):529–539. doi: 10.1042/bj1330529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Couroucé-Pauty A. M., Soulier J. P. Further data on HBs antigen subtypes - geographical distribution. Vox Sang. 1974;27(6):533–549. doi: 10.1111/j.1423-0410.1974.tb02451.x. [DOI] [PubMed] [Google Scholar]
  5. Dane D. S., Cameron C. H., Briggs M. Virus-like particles in serum of patients with Australia-antigen-associated hepatitis. Lancet. 1970 Apr 4;1(7649):695–698. doi: 10.1016/s0140-6736(70)90926-8. [DOI] [PubMed] [Google Scholar]
  6. Dreesman G. R., Chairez R., Suarez M., Hollinger F. B., Courtney R. J., Melnick J. L. Production of antibody to individual polypeptides derived from purified hepatitis B surface antigen. J Virol. 1975 Sep;16(3):508–515. doi: 10.1128/jvi.16.3.508-515.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fraker P. J., Speck J. C., Jr Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril. Biochem Biophys Res Commun. 1978 Feb 28;80(4):849–857. doi: 10.1016/0006-291x(78)91322-0. [DOI] [PubMed] [Google Scholar]
  8. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Galibert F., Mandart E., Fitoussi F., Tiollais P., Charnay P. Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli. Nature. 1979 Oct 25;281(5733):646–650. doi: 10.1038/281646a0. [DOI] [PubMed] [Google Scholar]
  10. Gerlich W., Thomssen R. Standardized detection of hepatitis B surface antigen: determination of its serum concentration in weight units per volume. Dev Biol Stand. 1975;30:78–87. [PubMed] [Google Scholar]
  11. Gold J. W., Shih J. W., Purcell R. H., Gerin J. L. Characterization of antibodies to the structural polypeptides of HGSAg: evidence for subtype-specific determinants. J Immunol. 1976 Oct;117(4):1404–1406. [PubMed] [Google Scholar]
  12. Holland P. V. Practical problems for HBSAg subtyping. Bibl Haematol. 1976;42:1–11. doi: 10.1159/000398977. [DOI] [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Le Bouvier G. L. The heterogeneity of Australia antigen. J Infect Dis. 1971 Jun;123(6):671–675. doi: 10.1093/infdis/123.6.671. [DOI] [PubMed] [Google Scholar]
  15. Marion P. L., Oshiro L. S., Regnery D. C., Scullard G. H., Robinson W. S. A virus in Beechey ground squirrels that is related to hepatitis B virus of humans. Proc Natl Acad Sci U S A. 1980 May;77(5):2941–2945. doi: 10.1073/pnas.77.5.2941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Marion P. L., Salazar F. H., Alexander J. J., Robinson W. S. Polypeptides of hepatitis B virus surface antigen produced by a hepatoma cell line. J Virol. 1979 Dec;32(3):796–802. doi: 10.1128/jvi.32.3.796-802.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Means G. E., Feeney R. E. Reductive alkylation of amino groups in proteins. Biochemistry. 1968 Jun;7(6):2192–2201. doi: 10.1021/bi00846a023. [DOI] [PubMed] [Google Scholar]
  18. Peterson D. L., Roberts I. M., Vyas G. N. Partial amino acid sequence of two major component polypeptides of hepatitis B surface antigen. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1530–1534. doi: 10.1073/pnas.74.4.1530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Robinson W. S. The genome of hepatitis B virus. Annu Rev Microbiol. 1977;31:357–377. doi: 10.1146/annurev.mi.31.100177.002041. [DOI] [PubMed] [Google Scholar]
  20. Schober A., Biswas R. M., Thomssen R. Direct solid-phase radioimmunoassay (dSP-RIA) for detection of antibody to hepatitis B surface (HBs) antigen. Dev Biol Stand. 1975;30:93–102. [PubMed] [Google Scholar]
  21. Shih J. W., Gerin J. L. Immunochemistry of hepatitis B surface antigen (HBsAg): preparation and characterization of antibodies to the constituent polypeptides. J Immunol. 1975 Sep;115(3):634–639. [PubMed] [Google Scholar]
  22. Shih J. W., Gerin J. L. Proteins of hepatitis B surface antigen. J Virol. 1977 Jan;21(1):347–357. doi: 10.1128/jvi.21.1.347-357.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shih J. W., Gerin J. L. Proteins of hepatitis B surface antigen: amino acid compositions of the major polypeptides. J Virol. 1977 Mar;21(3):1219–1222. doi: 10.1128/jvi.21.3.1219-1222.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Summers J., Smolec J. M., Snyder R. A virus similar to human hepatitis B virus associated with hepatitis and hepatoma in woodchucks. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4533–4537. doi: 10.1073/pnas.75.9.4533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Swanstrom R., Shank P. R. X-Ray Intensifying Screens Greatly Enhance the Detection by Autoradiography of the Radioactive Isotopes 32P and 125I. Anal Biochem. 1978 May;86(1):184–192. doi: 10.1016/0003-2697(78)90333-0. [DOI] [PubMed] [Google Scholar]
  26. Vyas G. N., Rao K. R., Ibrahim A. B. Australia antigen (hepatitis B antigen): a conformational antigen dependent on disulfide bonds. Science. 1972 Dec 22;178(4067):1300–1301. doi: 10.1126/science.178.4067.1300. [DOI] [PubMed] [Google Scholar]
  27. Vyas G. N., Williams E. W., Klaus G. G., Bond H. E. Hepatitis-associated Australia antigen. Protein, peptides and amine acid composition of purified antigen with its use in determining sensitivity of the hemagglutination test. J Immunol. 1972 Apr;108(4):1114–1118. [PubMed] [Google Scholar]
  28. Werner B. G., Smolec J. M., Snyder R., Summers J. Serological relationship of woodchuck hepatitis virus to human hepatitis B virus. J Virol. 1979 Oct;32(1):314–322. doi: 10.1128/jvi.32.1.314-322.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Yang R. C., Young A., Wu R. BK virus DNA sequence coding for the t and T antigens and evaluation of methods for determining sequence homology. J Virol. 1980 May;34(2):416–430. doi: 10.1128/jvi.34.2.416-430.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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