Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1980 Dec;36(3):872–877. doi: 10.1128/jvi.36.3.872-877.1980

Structure of and alterations to defective murine sarcoma virus particles lacking envelope proteins and core polyprotein cleavage.

A Demsey, F Collins, D Kawka
PMCID: PMC353714  PMID: 6257930

Abstract

HTG2 hamster cells produce a defective murine sarcoma virus lacking gp70 and, consequently, viral surface projections (knobs), but the lack of knobs appears to have no effect on intramembrane particle distribution. In addition, it has been noted that the core of the virus remains in the "immature" form as a result of the failure of the polyprotein precursor (p65) to undergo cleavage. However, incubation of HTG2 virus with avian myoblastosis virus was found to yield specific cleavage products of p65.

Full text

PDF
873

Images in this article

873Image
on p.873
874Image
on p.874
874Image
on p.874
874Image
on p.874
874Image
on p.874
876Image
on p.876

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blough H. A., Tiffany J. M. Theoretical aspects of structure and assembly of viral envelops. Curr Top Microbiol Immunol. 1975;70:1–30. doi: 10.1007/978-3-642-66101-3_1. [DOI] [PubMed] [Google Scholar]
  2. Bolognesi D. P., Montelaro R. C., Frank H., Schäfer W. Assembly of type C oncornaviruses: a model. Science. 1978 Jan 13;199(4325):183–186. doi: 10.1126/science.202022. [DOI] [PubMed] [Google Scholar]
  3. Demsey A., Kawka D., Galuska S., Stackpole C. W. Assembly of a temperature-sensitive mutant of Rauscher murine leukemia virus at the cell surface induced by low temperature and by ligands. Virology. 1979 May;95(1):235–240. doi: 10.1016/0042-6822(79)90422-7. [DOI] [PubMed] [Google Scholar]
  4. Demsey A., Kawka D., Stackpole C. W. Application of freeze-drying intact cells to studies of murine oncornavirus morphogenesis. J Virol. 1977 Jan;21(1):358–365. doi: 10.1128/jvi.21.1.358-365.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Demsey A., Kawka D., Stackpole C. W. Cell surface membrane organization revealed by freeze-drying. J Ultrastruct Res. 1978 Jan;62(1):13–25. doi: 10.1016/s0022-5320(78)80027-6. [DOI] [PubMed] [Google Scholar]
  6. Dittmar K. J., Moelling K. Biochemical properties of p15-associated protease in an avian RNA tumor virus. J Virol. 1978 Oct;28(1):106–118. doi: 10.1128/jvi.28.1.106-118.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Khan A. S., Stephenson J. R. Feline sarcoma virus-coded polyprotein: enzymatic cleavage by a type C virus-coded structural protein. J Virol. 1979 Feb;29(2):649–656. doi: 10.1128/jvi.29.2.649-656.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Klenk H. D. Viral envelopes and their relationship to cellular membranes. Curr Top Microbiol Immunol. 1974;(68):29–58. doi: 10.1007/978-3-642-66044-3_2. [DOI] [PubMed] [Google Scholar]
  9. Marcus S. L., Smith S. W., Racevskis J., Sarkar N. H. The relative hydrophobicity of oncornaviral structural proteins. Virology. 1978 May 15;86(2):398–412. doi: 10.1016/0042-6822(78)90080-6. [DOI] [PubMed] [Google Scholar]
  10. Moelling K., Scott A., Dittmar K. E., Owada M. Effect of p15-associated protease from an avian RNA tumor virus on avian virus-specific polyprotein precursors. J Virol. 1980 Feb;33(2):680–688. doi: 10.1128/jvi.33.2.680-688.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Oskarsson M. K., Elder J. H., Gautsch J. W., Lerner R. A., Vande Woude G. F. Chemical determination of the m1 Moloney sarcoma virus pP60gag gene order: evidence for unique peptides in the carboxy terminus of the polyprotein. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4694–4698. doi: 10.1073/pnas.75.10.4694. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pepinsky R. B., Vogt V. M. Identification of retrovirus matrix proteins by lipid-protein cross-linking. J Mol Biol. 1979 Jul 15;131(4):819–837. doi: 10.1016/0022-2836(79)90203-1. [DOI] [PubMed] [Google Scholar]
  13. Pinter A., Fleissner E. Structural studies of retroviruses: characterization of oligomeric complexes of murine and feline leukemia virus envelope and core components formed upon cross-linking. J Virol. 1979 Apr;30(1):157–165. doi: 10.1128/jvi.30.1.157-165.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pinter A., deHarven E. Protein composition of a defective murine sarcoma virus particle possessing the enveloped type-A morphology. Virology. 1979 Nov;99(1):103–110. doi: 10.1016/0042-6822(79)90041-2. [DOI] [PubMed] [Google Scholar]
  15. Racevskis J., Sarkar N. H. Synthesis and processing of precursor polypeptides to murine mammary tumor virus structural proteins. J Virol. 1978 Jan;25(1):374–383. doi: 10.1128/jvi.25.1.374-383.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sheffield J. B. Membrane alterations which accompany MuMTV maturation. I. Studies by freeze-cleave techniques. Virology. 1974 Jan;57(1):287–290. doi: 10.1016/0042-6822(74)90130-5. [DOI] [PubMed] [Google Scholar]
  17. Yoshinaka Y., Luftig R. B. Characterization of Rauscher Leukemia virus (RLV) P40-42, an intermediate cleavage product of the group specific antigen (gag) precursor polypeptide, P65-70. Biochem Biophys Res Commun. 1977 Nov 7;79(1):319–325. doi: 10.1016/0006-291x(77)90098-5. [DOI] [PubMed] [Google Scholar]
  18. Yoshinaka Y., Luftig R. B. Partial characterization of a P70 proteolytic factor that is present in purified virions of Rauscher leukemia virus (RLV). Biochem Biophys Res Commun. 1977 May 9;76(1):54–63. doi: 10.1016/0006-291x(77)91667-9. [DOI] [PubMed] [Google Scholar]
  19. Yoshinaka Y., Luftig R. B. Properties of a P70 proteolytic factor of murine leukemia viruses. Cell. 1977 Nov;12(3):709–719. doi: 10.1016/0092-8674(77)90271-9. [DOI] [PubMed] [Google Scholar]
  20. de Giuli C., Kawai S., Dales S., Hanafusa H. Absence of surface projections of some noninfectious forms of RSV. Virology. 1975 Jul;66(1):253–260. doi: 10.1016/0042-6822(75)90195-6. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES