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. 1977 Jan;21(1):215–224. doi: 10.1128/jvi.21.1.215-224.1977

Cell-free synthesis of polyoma virus capsid proteins VP1 and VP2.

T Wheeler, S T Bayley, R Harvey, L V Crawford, A E Smith
PMCID: PMC353807  PMID: 189065

Abstract

Polyadenylated RNA isolated from the cytoplasm of mouse 3T6 cells 28 h after infection with polyoma virus has been isolated and translated in vitro. Polyoma capsid proteins VP1 and VP2 have been identified in the cell-free product by polyacrylamide gel electrophoresis, specific immunoprecipitation, and tryptic peptide fingerprinting. Polyoma mRNA species have been isolated by preparative hybridization to purified viral DNA immobilized on cellulose nitrate filters and shown to code for both VP1 and VP2. These experiments establish conditions for the isolation of late polyoma mRNA and the cell-free synthesis of polyoma capsid proteins and indicate that the active mRNA species are at least partially virus coded.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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