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. 1977 Jan;21(1):225–231. doi: 10.1128/jvi.21.1.225-231.1977

Biochemical, biophysical, and biological properties of densonucleosis virus (paravovirus). II. Two types of infectious virions.

P Tijssen, T Tijssen-van der Slikke, E Kurstak
PMCID: PMC353808  PMID: 833923

Abstract

Among the infectious densonucleosis virus (DNV) particles, two types of virions, DNV-I and DNV-II, have been identified. The density of DNV-I was about 1.40 g/ml and depended on the pH, whereas the density of DNV-II was independent of the pH and was 1.44 g/ml. The sedimentation rates of these particles were 111S and 89S, respectively. The specific extinction coefficients and the absorption spectra, corrected for light scattering, were also different for these two types. The electrophoretic mobility of both types of virions was identical under the experimental conditions used. Both types of particles contained single-stranded DNA with similar physicochemical properties. The difference between these types of virions seemed to be due to a different protein content, DNV-II containing about 600,000 daltons less protein, and to a different quaternary structure as indicated by the change in the density of DNV-II, in the presence of divalent cations, without any change in the density of DNV-I.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann P. A., Hoggan M. D., Melnick J. L., Pereira H. G., Vago C. Parvoviridae. Intervirology. 1975;5(1-2):83–92. doi: 10.1159/000149884. [DOI] [PubMed] [Google Scholar]
  2. Berns K. I. Molecular biology of the adeno-associated viruses. Curr Top Microbiol Immunol. 1974;65:1–20. doi: 10.1007/978-3-642-65875-4_1. [DOI] [PubMed] [Google Scholar]
  3. Clinton G. M., Hayashi M. The parovivirus MVM: particles with altered structural proteins. Virology. 1975 Jul;66(1):261–261. doi: 10.1016/0042-6822(75)90196-8. [DOI] [PubMed] [Google Scholar]
  4. Gibson W., Roizman B. Proteins specified by herpes simplex virus. 8. Characterization and composition of multiple capsid forms of subtypes 1 and 2. J Virol. 1972 Nov;10(5):1044–1052. doi: 10.1128/jvi.10.5.1044-1052.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Johnson F. B., Ozer H. L., Hoggan M. D. Structural proteins of adenovirus-associated virus type 3. J Virol. 1971 Dec;8(6):860–863. doi: 10.1128/jvi.8.6.860-863.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Johnson F. B., Whitaker C. W., Hoggan M. D. Structural polypeptides of adenovirus-associated virus top component. Virology. 1975 May;65(1):196–203. doi: 10.1016/0042-6822(75)90020-3. [DOI] [PubMed] [Google Scholar]
  7. Kaper J. M., Geelen J. L. Studies on the stabilizing forces of simple RNA viruses. II. Stability, dissociation and reassembly of cucumber mosaic virus. J Mol Biol. 1971 Mar 14;56(2):277–294. doi: 10.1016/0022-2836(71)90464-5. [DOI] [PubMed] [Google Scholar]
  8. Klug A., Longley W., Leberman R. Arrangement of protein subunits and the distribution of nucleic acid in turnip yellow mosaic virus. I. X-ray diffraction studies. J Mol Biol. 1966 Jan;15(1):315–343. doi: 10.1016/s0022-2836(66)80230-9. [DOI] [PubMed] [Google Scholar]
  9. Koczot F. J., Carter B. J., Garon C. F., Rose J. A. Self-complementarity of terminal sequences within plus or minus strands of adenovirus-associated virus DNA. Proc Natl Acad Sci U S A. 1973 Jan;70(1):215–219. doi: 10.1073/pnas.70.1.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kurstak E. Small DNA densonucleosis virus (DNV). Adv Virus Res. 1972;17:207–241. doi: 10.1016/s0065-3527(08)60751-4. [DOI] [PubMed] [Google Scholar]
  11. Kurstak E., Vernoux J. P., Brakier-Gingras L. Etude biophysique de l'acide désoxyribonucléique de virus de la densonucléose (VDN). II. Extraction du DNA viral et mise en évidence de la présence de chaînes polynucléotidiques complémentairs, encapsidées séparément dans les virions VD. Arch Gesamte Virusforsch. 1973;40(3):274–284. [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. MAYOR H. D., HILL N. O. Acridine orange staining of a single-stranded DNA bacteriophage. Virology. 1961 Jun;14:264–266. doi: 10.1016/0042-6822(61)90202-1. [DOI] [PubMed] [Google Scholar]
  14. Tijssen P., van den Hurk J., Kurstak E. Biochemical, biophysical, and biological properties of densonucleosis virus. I. Structural proteins. J Virol. 1976 Mar;17(3):686–691. doi: 10.1128/jvi.17.3.686-691.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tinsley T. W., Longworth J. F. Parvoviruses. J Gen Virol. 1973 Jun;20(Suppl):7–15. doi: 10.1099/0022-1317-20-Supplement-7. [DOI] [PubMed] [Google Scholar]
  16. Truffaut N., Berger G., Niveleau A., May P., Bergoin M., Vago C. Recherches sur l'acide nucléique du virus de la densonucléose du lepidoptère Galleria mellonella L. Arch Gesamte Virusforsch. 1967;21(3):469–474. [PubMed] [Google Scholar]

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