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. 1977 Feb;21(2):493–496. doi: 10.1128/jvi.21.2.493-496.1977

Bacillus subtilis DNA polymerase III is required for the replication of DNA of bacteriophages SPP-1 and phi 105.

S D Rowley, N C Brown
PMCID: PMC353849  PMID: 401898

Abstract

The replication of the Bacillus subtilis bacteriophages SPP-1 and phi 105 is sensitive to 6-(p-hydroxyphenylazo)-uracil (HPUra), a selective inhibitor of replicative DNA synthesis of B. subtilis which acts specifically at the levels of a replication-specific polymerase, DNA polymerase III (pol III). The origin of the HPUra-sensitive polymerase required for phage replication was examined by comparison of the drug sensitivity of phage development in a normosensitive host with that in a host carrying azp-12, a polC mutation that specifies production of an HPUra-resistant pol III. azp-12 specified HPUra-resistant phage host pol III. The host polIII requirement for SPP-1 replication also was confirmed by the demonstration that phage development was temperature sensitive in a host mutant carrying the polC mutation mut-1 (ts). Examination of the pol III activity of crude and purified cell-free preparations derived from phage-infected cells did not indicate any detectable changes in the specific activity, purification behavior, or drug sensitivity of the enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anagnostopoulos C., Spizizen J. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961 May;81(5):741–746. doi: 10.1128/jb.81.5.741-746.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bazill G. W., Gross J. D. Effect of 6-(p-hydroxyphenyl)-azouracil on B. subtilis DNA polymerases. Nat New Biol. 1972 Nov 15;240(98):82–83. doi: 10.1038/newbio240082a0. [DOI] [PubMed] [Google Scholar]
  3. Bazill G. W., Gross J. D. Mutagenic DNA polymerase in B. subtilis. Nat New Biol. 1973 Jun 20;243(129):241–243. doi: 10.1038/newbio243241a0. [DOI] [PubMed] [Google Scholar]
  4. Brown N. C. 6-(p-hydroxyphenylazo)-uracil: a selective inhibitor of host DNA replication in phage-infected Bacillus subtilis. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1454–1461. doi: 10.1073/pnas.67.3.1454. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brown N. C. Inhibition of bacterial DNA replication by 6-(p-hydroxyphenylazo)-uracil: differential effect on repair and semi-conservative synthesis in Bacillus subtilis. J Mol Biol. 1971 Jul 14;59(1):1–16. doi: 10.1016/0022-2836(71)90409-8. [DOI] [PubMed] [Google Scholar]
  6. Clements J. E., D'Ambrosio J., Brown N. C. Inhibition of Bacillus subtilis deoxyribonucleic acid polymerase III by phenylhydrazinopyrimidines. Demonstration of a drug-induced deoxyribonucleic acid-enzyme complex. J Biol Chem. 1975 Jan 25;250(2):522–526. [PubMed] [Google Scholar]
  7. Cozzarelli N. R., Low R. L. Mutational alteration of Bacillus subtilis DNA polymerase 3 to hydroxyphenylazopyrimidine resistance: polymerase 3 is necessary for DNA replication. Biochem Biophys Res Commun. 1973 Mar 5;51(1):151–157. doi: 10.1016/0006-291x(73)90521-4. [DOI] [PubMed] [Google Scholar]
  8. Esche H. Gene expression of bacteriophage SPP1. II. Regulatory aspects. Mol Gen Genet. 1975 Dec 23;142(1):57–66. doi: 10.1007/BF00268755. [DOI] [PubMed] [Google Scholar]
  9. FARMER J. L., ROTHMAN F. TRANSFORMABLE THYMINE-REQUIRING MUTANT OF BACILLUS SUBTILS. J Bacteriol. 1965 Jan;89:262–263. doi: 10.1128/jb.89.1.262-263.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gass K. B., Cozzarelli N. R. Further genetic and enzymological characterization of the three Bacillus subtilis deoxyribonucleic acid polymerases. J Biol Chem. 1973 Nov 25;248(22):7688–7700. [PubMed] [Google Scholar]
  11. Gass K. B., Low R. L., Cozzarelli N. R. Inhibition of a DNA polymerase from Bacillus subtilis by hydroxyphenylazopyrimidines. Proc Natl Acad Sci U S A. 1973 Jan;70(1):103–107. doi: 10.1073/pnas.70.1.103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lavi U., Nattenberg A., Ronen A., Marcus M. Bacillus subtilis DNA polymerase III is required for the replication of the virulent bacteriophage phi e. J Virol. 1974 Dec;14(6):1337–1342. doi: 10.1128/jvi.14.6.1337-1342.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Love E., D'Ambrosio D., Brown N. C. Mapping of the gene specifying DNA polymerase III of Bacillus subtilis. Mol Gen Genet. 1976 Mar 30;144(3):313–321. doi: 10.1007/BF00341730. [DOI] [PubMed] [Google Scholar]
  14. Low R. L., Rashbaum S. A., Cozzarelli N. R. Purification and characterization of DNA polymerase III from Bacillus subtilis. J Biol Chem. 1976 Mar 10;251(5):1311–1325. [PubMed] [Google Scholar]
  15. Mackenzie J. M., Neville M. M., Wright G. E., Brown N. C. Hydroxyphenylazopyrimidines: characterization of the active forms and their inhibitory action on a DNA polymerase from Bacillus subtilis. Proc Natl Acad Sci U S A. 1973 Feb;70(2):512–516. doi: 10.1073/pnas.70.2.512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Neville M. M., Brown N. C. Inhibition of a discrete bacterial DNA polymerase by 6-(p-hydroxyphenylazo)-uracil and 6-(p-hydroxyphenylazo-)-isocytosine. Nat New Biol. 1972 Nov 15;240(98):80–82. doi: 10.1038/newbio240080a0. [DOI] [PubMed] [Google Scholar]
  17. OKAZAKI T., KORNBERG A. ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XV. PURIFICATION AND PROPERTIES OF A POLYMERASE FROM BACILLUS SUBTILIS. J Biol Chem. 1964 Jan;239:259–268. [PubMed] [Google Scholar]
  18. Riva S., Polsinelli M., Falaschi A. A new phage of Bacillus subtilis with infectious DNA having separable strands. J Mol Biol. 1968 Jul 28;35(2):347–356. doi: 10.1016/s0022-2836(68)80029-4. [DOI] [PubMed] [Google Scholar]
  19. Rohrer H., Zillig W., Mailhammer R. ADP-ribosylation of DNA-dependent RNA polymerase of Escherichia coli by an NAD+: protein ADP-ribosyltransferase from bacteriophage T4. Eur J Biochem. 1975 Dec 1;60(1):227–238. doi: 10.1111/j.1432-1033.1975.tb20995.x. [DOI] [PubMed] [Google Scholar]
  20. Rutberg L., Armentrout R. W., Jonasson J. Unrelatedness of temperate Bacillus subtilis bacteriophages SP02 and phi105. J Virol. 1972 May;9(5):732–737. doi: 10.1128/jvi.9.5.732-737.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yasbin R. E., Young F. E. Transduction in Bacillus subtilis by bacteriophage SPP1. J Virol. 1974 Dec;14(6):1343–1348. doi: 10.1128/jvi.14.6.1343-1348.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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