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. 1978 Feb;25(2):546–552. doi: 10.1128/jvi.25.2.546-552.1978

Protein kinase from avian myeloblastosis virus.

G E Houts, M Miyagi, C Ellis, D Beard, K F Watson, J W Beard
PMCID: PMC353967  PMID: 24125

Abstract

A protein kinase associated with purified virions of avian myeloblastosis BAI strain A was partially purified by ion-exchange chromatography and gel filtration. The transfer of phosphate catalyzed by this enzyme required a divalent metal ion and ATP as phosphate donor. GTP could not be substituted for ATP, and the reaction was unaffected by either cyclic AMP or beef-heart protein-kinase inhibitor. Of the virus and nonvirus proteins tested as phosphate acceptors, only acidic proteins were phosphorylated. In particular, purified preparations of reverse transcriptase from avian myeloblastosis virus did not accept phosphate. The enzyme is a basic protein (pI = 9.3), and, on the basis of molecular sieving through Sephadex G-200 and velocity sedimentation on glycerol gradient, the protein kinase has a molecular weight of 45,000.

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Selected References

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  1. BEAUDREAU G. S., BECKER C. Virus of avian myeloblastosis. X. Photometric microdetermination of adenosinetriphosphatase activity. J Natl Cancer Inst. 1958 Feb;20(2):339–349. [PubMed] [Google Scholar]
  2. Efstratiadis A., Maniatis T., Kafatos F. C., Jeffrey A., Vournakis J. N. Full length and discrete partial reverse transcripts of globin and chorion mRNAs. Cell. 1975 Apr;4(4):367–378. doi: 10.1016/0092-8674(75)90157-9. [DOI] [PubMed] [Google Scholar]
  3. Gravell M., Cromeans T. L. Viron-associated protein kinase and its involvement in nongenetic reactivation of frog polyhedral cytoplasmic deoxyribovirus. Virology. 1972 Jun;48(3):847–851. doi: 10.1016/0042-6822(72)90167-5. [DOI] [PubMed] [Google Scholar]
  4. Hatanaka M., Twiddy E., Gilden R. V. Protein kinase associated with RNA tumor viruses and other budding RNA viruses. Virology. 1972 Feb;47(2):536–538. doi: 10.1016/0042-6822(72)90297-8. [DOI] [PubMed] [Google Scholar]
  5. Hizi A., Joklik W. K. The beta subunit of the DNA polymerase of avian sarcoma virus strain B77 is a phosphoprotein. Virology. 1977 May 15;78(2):571–575. doi: 10.1016/0042-6822(77)90132-5. [DOI] [PubMed] [Google Scholar]
  6. Imblum R. L., Wagner R. R. Protein kinase and phosphoproteins of vesicular stomatitis virus. J Virol. 1974 Jan;13(1):113–124. doi: 10.1128/jvi.13.1.113-124.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kacian D. L., Spiegelman S. Purification and detection of reverse transcriptase in viruses and cells. Methods Enzymol. 1974;29:150–173. doi: 10.1016/0076-6879(74)29018-9. [DOI] [PubMed] [Google Scholar]
  8. Kleiman J. H., Moss B. Characterization of a protein kinase and two phosphate acceptor proteins from vaccinia virions. J Biol Chem. 1975 Apr 10;250(7):2430–2437. [PubMed] [Google Scholar]
  9. Kleiman J. H., Moss B. Purification of a protein kinase and two phosphate acceptor proteins from vaccinia virions. J Biol Chem. 1975 Apr 10;250(7):2420–2429. [PubMed] [Google Scholar]
  10. Lee S. G., Miceli M. V., Jungmann R. A., Hung P. P. Protein kinase and its regulatory effect on reverse transcriptase activity of Rous sarcoma virus. Proc Natl Acad Sci U S A. 1975 Aug;72(8):2945–2949. doi: 10.1073/pnas.72.8.2945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Monahan J. J., Harris S. E., Woo S. L., Robberson D. L., O'Malley B. W. The synthesis and properties of the complete complementary DNA transcript of ovalbumin mRNA. Biochemistry. 1976 Jan 13;15(1):223–233. doi: 10.1021/bi00646a034. [DOI] [PubMed] [Google Scholar]
  12. Paoletti E., Moss B. Protein kinase and specific phosphate acceptor proteins associated with vaccinia virus cores. J Virol. 1972 Sep;10(3):417–424. doi: 10.1128/jvi.10.3.417-424.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rabbitts T. H. Bacterial cloning of plasmids carrying copies of rabbit globin messenger RNA. Nature. 1976 Mar 18;260(5548):221–225. doi: 10.1038/260221a0. [DOI] [PubMed] [Google Scholar]
  14. Randall C. C., Rogers H. W., Downer D. N., Gentry G. A. Protein kinase activity in equine herpesvirus. J Virol. 1972 Feb;9(2):216–222. doi: 10.1128/jvi.9.2.216-222.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Roux L., Kolakofsky D. Protein kinase associated with Sendai virions. J Virol. 1974 Feb;13(2):545–547. doi: 10.1128/jvi.13.2.545-547.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rubenstein A. S., Gravell M., Darlington R. Protein kinase in enveloped herpes simplex virions. Virology. 1972 Oct;50(1):287–290. doi: 10.1016/0042-6822(72)90374-1. [DOI] [PubMed] [Google Scholar]
  17. Silberstein H., August J. T. Characterization of a virion protein kinase as a virus-specified enzyme. J Biol Chem. 1976 May 25;251(10):3185–3190. [PubMed] [Google Scholar]
  18. Silberstein H., August J. T. Phosphorylation of animal virus proteins by a virion protein kinase. J Virol. 1973 Sep;12(3):511–522. doi: 10.1128/jvi.12.3.511-522.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Silberstein H., August J. T. Purification and properties of a virion protein kinase. J Biol Chem. 1976 May 25;251(10):3176–3184. [PubMed] [Google Scholar]
  20. Strand M., August J. T. Protein kinase and phosphate acceptor proteins in Rauscher murine leukaemia virus. Nat New Biol. 1971 Sep 29;233(39):137–140. doi: 10.1038/newbio233137a0. [DOI] [PubMed] [Google Scholar]
  21. Taborsky G. Phosphoproteins. Adv Protein Chem. 1974;28:1–210. doi: 10.1016/s0065-3233(08)60230-2. [DOI] [PubMed] [Google Scholar]
  22. Tan K. B. Comparative study of the protein kinase associated with animal viruses. Virology. 1975 Apr;64(2):566–570. doi: 10.1016/0042-6822(75)90135-x. [DOI] [PubMed] [Google Scholar]
  23. Tan K. B., Sokol F. Virion-bound protein kinase in Semliki forest and Sindbis viruses. J Virol. 1974 Jun;13(6):1245–1253. doi: 10.1128/jvi.13.6.1245-1253.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tsiapalis C. M. Chemical modification of DNA polymerase phosphoprotein from avian myeloblastosis virus. Nature. 1977 Mar 3;266(5597):27–31. doi: 10.1038/266027a0. [DOI] [PubMed] [Google Scholar]
  25. Tsiapalis C. M. Protein kinase and phosphoproteins of avian myeloblastosis virus. J Virol. 1977 Mar;21(3):843–848. doi: 10.1128/jvi.21.3.843-848.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Waite M. R., Lubin M., Jones K. J., Bose H. R. Phosphorylated proteins of Sindbis virus. J Virol. 1974 Jan;13(1):244–246. doi: 10.1128/jvi.13.1.244-246.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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