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. 1978 Feb;25(2):570–578. doi: 10.1128/jvi.25.2.570-578.1978

Polyoma virus complementary RNA directs the in vitro synthesis of capsid proteins VP1 and VP2.

W F Mangel, R M Hewick, S T Bayley, T Wheeler, R Harvey, M D Waterfield, A E Smith
PMCID: PMC353970  PMID: 203720

Abstract

Polyoma virus complementary RNA, synthesized in vitro by using highly purified Escherichia coli RNA polymerase and nondefective form I polyoma DNA, was translated in a wheat germ cell-free system. Polypeptides were synthesized that comigrated on sodium dodecyl sulfate-polyacrylamide gels with the polyoma capsid proteins VP1 and VP2, although most of the cell-free products were of smaller molecular weights. The VP1-size protein specifically immunoprecipitated with anti-polyoma virus serum, and upon digestion by trypsin yielded [35S]methionine-labeled tryptic peptides that co-chromatographed with the [3H]methionine-labeled tryptic peptides of virion-derived VP1 on both cation-exchange and anion-exchange resins. The VP2-size in vitro product contained all the virion VP2 methionine-labeled tryptic peptides, as shown by cation- and anion-exchange chromatography and two-dimensional fingerprinting on cellulose. We conclude that full-length polyoma VP1 and VP2 are synthesized in response to complementary RNA and consequently that the viral capsid proteins VP1, VP2, and VP3 are entirely virus coded.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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