Abstract
It was possible to transfect Streptococcus pneumoniae with DNA obtained from a newly isolated bacteriophage, diplophage-4 (Dp-4). Optimal frequency of transfection (0.9%) required the use of a nuclease-defective mutant; with wild-type bacteria, the transfection frequency was about 100-fold lower. Transfection requires physiological conditions that appear to be similar to the competent state needed for genetic transformation (A. Tomasz, J. Bacteriol. 91:1050--1061, 1966).
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Benzinger R., Enquist L. W., Skalka A. Transfection of Escherichia coli spheroplasts. V. Activity of recBC nuclease in rec+ and rec minus spheroplasts measured with different forms of bacteriophage DNA. J Virol. 1975 Apr;15(4):861–871. doi: 10.1128/jvi.15.4.861-871.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bradley D. E. A comparative study of some properties of the phi-X174 type bacteriophages. Can J Microbiol. 1970 Oct;16(10):965–971. doi: 10.1139/m70-165. [DOI] [PubMed] [Google Scholar]
- HOTTA Y., BASSEL A. MOLECULAR SIZE AND CIRCULARITY OF DNA IN CELLS OF MAMMALS AND HIGHER PLANTS. Proc Natl Acad Sci U S A. 1965 Feb;53:356–362. doi: 10.1073/pnas.53.2.356. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Horne D. S., Perry D. Effect of competence induction on macromolecular synthesis in a group H streptococcus. J Bacteriol. 1974 Jun;118(3):830–836. doi: 10.1128/jb.118.3.830-836.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lacks S., Greenberg B., Neuberger M. Identification of a deoxyribonuclease implicated in genetic transformation of Diplococcus pneumoniae. J Bacteriol. 1975 Jul;123(1):222–232. doi: 10.1128/jb.123.1.222-232.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lopez R., Ronda C., Tomasz A., Portoles A. Properties of "diplophage": a lipid-containing bacteriophage. J Virol. 1977 Oct;24(1):201–210. doi: 10.1128/jvi.24.1.201-210.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mcdonnell M., Lain R., Tomasz A. "Diplophage": a bacteriophage of Diplococcus pneumoniae. Virology. 1975 Feb;63(2):577–582. doi: 10.1016/0042-6822(75)90329-3. [DOI] [PubMed] [Google Scholar]
- Parsons C. L., Cole R. M. Transfection of group H streptococci. J Bacteriol. 1973 Mar;113(3):1505–1506. doi: 10.1128/jb.113.3.1505-1506.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Porter R. D., Guild W. R. Characterization of some pneumococcal bacteriophages. J Virol. 1976 Aug;19(2):659–667. doi: 10.1128/jvi.19.2.659-667.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Riva S., Polsinelli M. Relationship between competence for transfection and for transformation. J Virol. 1968 Jun;2(6):587–593. doi: 10.1128/jvi.2.6.587-593.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sjöström J. E., Lindberg M., Philipson L. Transfection of Staphylococcus aureus with bacteriophage deoxyribonucleic acid. J Bacteriol. 1972 Jan;109(1):285–291. doi: 10.1128/jb.109.1.285-291.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spatz H. C., Trautner T. A. The role of recombination in transfection of B. subtilis. Mol Gen Genet. 1971;113(2):174–190. doi: 10.1007/BF00333191. [DOI] [PubMed] [Google Scholar]
- TOMASZ A., HOTCHKISS R. D. REGULATION OF THE TRANSFORMABILITY OF PHEUMOCOCCAL CULTURES BY MACROMOLECULAR CELL PRODUCTS. Proc Natl Acad Sci U S A. 1964 Mar;51:480–487. doi: 10.1073/pnas.51.3.480. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tiraby J. G., Tiraby E., Fox M. S. Pneumococcal bacteriophages. Virology. 1975 Dec;68(2):566–569. doi: 10.1016/0042-6822(75)90300-1. [DOI] [PubMed] [Google Scholar]
- Tomasz A. Cellular metabolism in genetic transformation of pneumococci: requirement for protein synthesis during induction of competence. J Bacteriol. 1970 Mar;101(3):860–871. doi: 10.1128/jb.101.3.860-871.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tomasz A. Model for the mechanism controlling the expression of competent state in Pneumococcus cultures. J Bacteriol. 1966 Mar;91(3):1050–1061. doi: 10.1128/jb.91.3.1050-1061.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tomasz A. Model for the mechanism controlling the expression of competent state in Pneumococcus cultures. J Bacteriol. 1966 Mar;91(3):1050–1061. doi: 10.1128/jb.91.3.1050-1061.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Trautner T. A., Spatz H. C. Transfection in B. subtilis. Curr Top Microbiol Immunol. 1973;62:61–88. doi: 10.1007/978-3-642-65772-6_3. [DOI] [PubMed] [Google Scholar]