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. 1978 May;26(2):221–225. doi: 10.1128/jvi.26.2.221-225.1978

Transfection of Streptococcus pneumoniae with bacteriophage DNA.

C Ronda, R Lopez, A Tomasz, A Portoles
PMCID: PMC354058  PMID: 26809

Abstract

It was possible to transfect Streptococcus pneumoniae with DNA obtained from a newly isolated bacteriophage, diplophage-4 (Dp-4). Optimal frequency of transfection (0.9%) required the use of a nuclease-defective mutant; with wild-type bacteria, the transfection frequency was about 100-fold lower. Transfection requires physiological conditions that appear to be similar to the competent state needed for genetic transformation (A. Tomasz, J. Bacteriol. 91:1050--1061, 1966).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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