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. 1975 Jan;15(1):115–120. doi: 10.1128/jvi.15.1.115-120.1975

Antigenic characterization of type C RNA virus isolates of gibbon apes.

S R Tronick, J R Stephenson, S A Aaronson, T G Kawakami
PMCID: PMC354424  PMID: 46280

Abstract

Type C RNA viruses initially isolated from a lymphosarcoma of a gibbon ape and from a fibrosarcoma of a woolly monkey are very closely related immunologically. However, recent studies have shown that these viruses are distinguishable in a radioimmunoassay for the 12,000-molecular-weight polypeptide (p12) of the woolly monkey virus. In the present report, an immunoassay has been developed for the p12 polypeptide of the gibbon ape type C virus. This assay is shown to further distinguish the woolly monkey and gibbon ape viruses. In type-specific assays for the p12 polypeptides of these viruses, two new type C viruses isolated from gibbons in a second colony, characterized by high incidence of hemopoietic neoplasia, are immunologically distinguishable from the original gibbon ape virus. The p12 type-specific immunoassays described in the present report may be of importance in studying the natural history of these viruses and their relationship to tumors of primates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A. Chemical induction of focus-forming virus from nonproducer cells transformed by murine sarcoma virus. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3069–3072. doi: 10.1073/pnas.68.12.3069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aaronson S. A., Hartley J. W., Todaro G. J. Mouse leukemia virus: "spontaneous" release by mouse embryo cells after long-term in vitro cultivation. Proc Natl Acad Sci U S A. 1969 Sep;64(1):87–94. doi: 10.1073/pnas.64.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Aaronson S. A., Todaro G. J., Scolnick E. M. Induction of murine C-type viruses from clonal lines of virus-free BALB-3T3 cells. Science. 1971 Oct 8;174(4005):157–159. doi: 10.1126/science.174.4005.157. [DOI] [PubMed] [Google Scholar]
  4. August J. T., Bolognesi D. P., Fleissner E., Gilden R. V., Nowinski R. C. A proposed nomenclature for the virion proteins of oncogenic RNA viruses. Virology. 1974 Aug;60(2):595–600. doi: 10.1016/0042-6822(74)90356-0. [DOI] [PubMed] [Google Scholar]
  5. Baluda M. A., Roy-Burman P. Partial characterization of RD114 virus by DNA-RNA hybridization studies. Nat New Biol. 1973 Jul 11;244(132):59–62. doi: 10.1038/newbio244059a0. [DOI] [PubMed] [Google Scholar]
  6. Benveniste R. E., Heinemann R., Wilson G. L., Callahan R., Todaro G. J. Detection of baboon type C viral sequences in various primate tissues by molecular hybridization. J Virol. 1974 Jul;14(1):56–67. doi: 10.1128/jvi.14.1.56-67.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Benveniste R. E., Todaro G. J. Homology between type-C viruses of various species as determined by molecular hybridization. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3316–3320. doi: 10.1073/pnas.70.12.3316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Chattopadhyay S. K., Lowy D. R., Teich N. M., Levine A. S., Rowe W. P. Evidence that the AKR murine-leukemia-virus genome is complete in DNA of the high-virus AKR mouse and incomplete in the DNA of the "virus-negative" NIH mouse. Proc Natl Acad Sci U S A. 1974 Jan;71(1):167–171. doi: 10.1073/pnas.71.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fischinger P. J., Peebles P. T., Nomura S., Haapala D. K. Isolation of RD-114-like oncornavirus from a cat cell line. J Virol. 1973 Jun;11(6):978–985. doi: 10.1128/jvi.11.6.978-985.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gallagher R. E., Todaro G. J., Smith R. G., Livingston D. M., Gallo R. C. Relationship between RNA-directed DNA polymerase (reverse transcriptase) from human acute leukemic blood cells and primate type-C viruses. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1309–1313. doi: 10.1073/pnas.71.4.1309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gelb L. D., Aaronson S. A., Martin M. A. Heterogeneity of murine leukemia virus in vitro DNA; detection of viral DNA in mammalian cells. Science. 1971 Jun 25;172(3990):1353–1355. doi: 10.1126/science.172.3990.1353. [DOI] [PubMed] [Google Scholar]
  13. Gelb L. D., Milstien J. B., Martin M. A., Aaronson S. A. Characterization of murine leukaemia virus-specific DNA present in normal mouse cells. Nat New Biol. 1973 Jul 18;244(133):76–79. doi: 10.1038/newbio244076a0. [DOI] [PubMed] [Google Scholar]
  14. Gilden R. V., Toni R., Hanson M., Bova D., Charman H. P., Oroszlan S. Immunochemical studies of the major internal polypeptide of woolly monkey and gibbon ape type C viruses. J Immunol. 1974 Mar;112(3):1250–1254. [PubMed] [Google Scholar]
  15. Gillespie D., Gillespie S., Gallo R. C., East J. L., Dmochowski L. Genetic origin of RD114 and other RNA tumour viruses assayed by molecular hybridization. Nat New Biol. 1973 Jul 11;244(132):51–54. doi: 10.1038/newbio244051a0. [DOI] [PubMed] [Google Scholar]
  16. Gregoriades A., Old L. J. Isolation and some characteristics of a group-specific antigen of the murine leukemia viruses. Virology. 1969 Feb;37(2):189–202. doi: 10.1016/0042-6822(69)90198-6. [DOI] [PubMed] [Google Scholar]
  17. Huu Duc-Nguyen, Rosenblum E. N., Zeigel R. F. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J Bacteriol. 1966 Oct;92(4):1133–1140. doi: 10.1128/jb.92.4.1133-1140.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kawakami T. G., Buckley P. M. Antigenic studies on gibbon type-C viruses. Transplant Proc. 1974 Jun;6(2):193–196. [PubMed] [Google Scholar]
  19. Kawakami T. G., Buckley P., Huff S., McKain D., Fielding H. A comparative study in vitro of a simian virus isolated from spontaneous woolly monkey fibrosarcoma and of a known feline fibrosarcoma virus. Bibl Haematol. 1973;39:236–243. doi: 10.1159/000427847. [DOI] [PubMed] [Google Scholar]
  20. Kawakami T. G., Huff S. D., Buckley P. M., Dungworth D. L., Synder S. P., Gilden R. V. C-type virus associated with gibbon lymphosarcoma. Nat New Biol. 1972 Feb 9;235(58):170–171. doi: 10.1038/newbio235170a0. [DOI] [PubMed] [Google Scholar]
  21. Kawakami T., Buckley P. M., McDowell T. S., DePaoli A. Antibodies to simian C-type virus antigen in sera of gibbons (Hylobates sp.). Nat New Biol. 1973 Nov 28;246(152):105–107. doi: 10.1038/newbio246105a0. [DOI] [PubMed] [Google Scholar]
  22. Klement V., Nicolson M. O., Huebner R. J. Rescue of the genome of focus forming virus from rat non-productive lines by 5'-bromodeoxyruidine. Nat New Biol. 1971 Nov 3;234(44):12–14. doi: 10.1038/newbio234012a0. [DOI] [PubMed] [Google Scholar]
  23. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  24. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  25. Livingston D. M., Todaro G. J. Endogenous type C virus from a cat cell clone with properties distinct from previously described feline type C virus. Virology. 1973 May;53(1):142–151. doi: 10.1016/0042-6822(73)90473-x. [DOI] [PubMed] [Google Scholar]
  26. Lowy D. R., Rowe W. P., Teich N., Hartley J. W. Murine leukemia virus: high-frequency activation in vitro by 5-iododeoxyuridine and 5-bromodeoxyuridine. Science. 1971 Oct 8;174(4005):155–156. doi: 10.1126/science.174.4005.155. [DOI] [PubMed] [Google Scholar]
  27. Neiman P. E. Measurement of RD114 virus nucleotide sequences in feline cellular DNA. Nat New Biol. 1973 Jul 11;244(132):62–64. doi: 10.1038/newbio244062a0. [DOI] [PubMed] [Google Scholar]
  28. Okabe H., Gilden R. V., Hatanaka M. Extensive homology of RD114 virus DNA with RNA of feline cell origin. Nat New Biol. 1973 Jul 11;244(132):54–56. doi: 10.1038/newbio244054a0. [DOI] [PubMed] [Google Scholar]
  29. Okabe H., Gilden R. V., Hatanaka M. Specificity of the DNA product of RNA-dependent DNA polymerase in type C viruses. II. Quantitative analysis. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3923–3927. doi: 10.1073/pnas.70.12.3923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Parks W. P., Scolnick E. M., Noon M. C., Watson C. J., Kawakami T. G. Radioimmunoassay of mammalian type-C polypeptides. IV. Characterization of woolly monkey and gibbon viral antigens. Int J Cancer. 1973 Jul 15;12(1):129–137. doi: 10.1002/ijc.2910120114. [DOI] [PubMed] [Google Scholar]
  31. Quintrell N., Varmus H. E., Bishop J. M., Nicholson M. O., McAllister R. M. Homologies among the nucleotide sequences of the genomes of C-type viruses. Virology. 1974 Apr;58(2):568–575. doi: 10.1016/0042-6822(74)90090-7. [DOI] [PubMed] [Google Scholar]
  32. Rangan S. R. Antigenic relatedness of simian C-type viruses. Int J Cancer. 1974 Jan 15;13(1):64–70. doi: 10.1002/ijc.2910130108. [DOI] [PubMed] [Google Scholar]
  33. Scolnick E. M., Parks W. P., Todaro G. J. Reverse transcriptases of primate viruses as immunological markers. Science. 1972 Sep 22;177(4054):1119–1121. doi: 10.1126/science.177.4054.1119. [DOI] [PubMed] [Google Scholar]
  34. Scolnick E. M., Parks W., Kawakami T., Kohne D., Okabe H., Gilden R., Hatanaka M. Primate and murine type-C viral nucleic acid association kinetics: analysis of model systems and natural tissues. J Virol. 1974 Feb;13(2):363–369. doi: 10.1128/jvi.13.2.363-369.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Snyder S. P., Dungworth D. L., Kawakami T. G., Callaway E., Lau D. T. Lymphosarcomas in two gibbons (Hylobates lar) with associated C-type virus. J Natl Cancer Inst. 1973 Jul;51(1):89–94. doi: 10.1093/jnci/51.1.89. [DOI] [PubMed] [Google Scholar]
  36. Stephenson J. R., Aaronson S. A. Expression of endogenous RNA C-type virus group-specific antigens in mammalian cells. J Virol. 1973 Sep;12(3):564–569. doi: 10.1128/jvi.12.3.564-569.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Stephenson J. R., Aaronson S. A. Genetic factors influencing C-type RNA virus induction. J Exp Med. 1972 Jul 1;136(1):175–184. doi: 10.1084/jem.136.1.175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Stephenson J. R., Tronick S. R., Aaronson S. A. Analysis of type specific antigenic determinants of two structural polypeptides of mouse RNA C-Type viruses. Virology. 1974 Mar;58(1):1–8. doi: 10.1016/0042-6822(74)90135-4. [DOI] [PubMed] [Google Scholar]
  39. Stephenson J. R., Tronick S. R., Aaronson S. A. Isolation from BALB/c mouse cells of a structural polypeptide of a third endogenous type C virus. Cell. 1974 Dec;3(4):347–353. doi: 10.1016/0092-8674(74)90049-x. [DOI] [PubMed] [Google Scholar]
  40. Theilen G. H., Gould D., Fowler M., Dungworth D. L. C-type virus in tumor tissue of a woolly monkey (Lagothrix spp.) with fibrosarcoma. J Natl Cancer Inst. 1971 Oct;47(4):881–889. [PubMed] [Google Scholar]
  41. Todaro G. J., Gallo R. C. Immunological relationship of DNA polymerase from human acute leukaemia cells and primate and mouse leukaemia virus reverse transcriptase. Nature. 1973 Jul 27;244(5413):206–209. doi: 10.1038/244206a0. [DOI] [PubMed] [Google Scholar]
  42. Tronick S. R., Stephenson J. R., Aaronson S. A. Comparative immunological studies of RNA C-type viruses: radioimmunoassay for a low molecular weight polypeptide of woolly monkey leukemia virus. Virology. 1974 Feb;57(2):347–356. doi: 10.1016/0042-6822(74)90174-3. [DOI] [PubMed] [Google Scholar]
  43. Tronick S. R., Stephenson J. R., Aaronson S. A. Immunological characterization of a low molecular weight polypeptide of murine leukemia virus. Virology. 1973 Jul;54(1):199–206. doi: 10.1016/0042-6822(73)90129-3. [DOI] [PubMed] [Google Scholar]
  44. Tronick S. R., Stephenson J. R., Aaronson S. A. Immunological properties of two polypeptides of Mason-Pfizer monkey virus. J Virol. 1974 Jul;14(1):125–132. doi: 10.1128/jvi.14.1.125-132.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. Weiss R. A., Friis R. R., Katz E., Vogt P. K. Induction of avian tumor viruses in normal cells by physical and chemical carcinogens. Virology. 1971 Dec;46(3):920–938. doi: 10.1016/0042-6822(71)90091-2. [DOI] [PubMed] [Google Scholar]

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