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Journal of Virology logoLink to Journal of Virology
. 1975 Feb;15(2):416–419. doi: 10.1128/jvi.15.2.416-419.1975

Alterations in Sindbis Viral Envelope Proteins by Treating BHK cells with Glucosamine

Ernö Duda a,1, Milton J Schlesinger a
PMCID: PMC354468  PMID: 1167605

Abstract

Addition of d-glucosamine to BHK cells infected with Sindbis virus inhibited the formation of the E-2 viral envelope from its precursor PE-2. Release of virus was blocked, and two new viral protein bands replaced the normal envelope protein bands detected in SDS-gel electropherograms of infected cell extracts.

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Selected References

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  1. Birdwell C. R., Strauss E. G., Strauss J. H. Replication of Sindbis virus. 3. An electron microscopic study of virus maturation using the surface replica technique. Virology. 1973 Dec;56(2):429–438. doi: 10.1016/0042-6822(73)90047-0. [DOI] [PubMed] [Google Scholar]
  2. Bosmann H. B. Inhibition of protein, glycoprotein, ribonucleic acid and deoxyribonucleic acid synthesis by D-glucosamine and other sugars in mouse leukemic cells L5178Y and selective inhibition in SV-3T3 compared with 3T3 cells. Biochim Biophys Acta. 1971 Jun 17;240(1):74–93. doi: 10.1016/0005-2787(71)90515-6. [DOI] [PubMed] [Google Scholar]
  3. Brown D. T., Waite M. R., Pfefferkorn E. R. Morphology and morphogenesis of Sindbis virus as seen with freeze-etching techniques. J Virol. 1972 Sep;10(3):524–536. doi: 10.1128/jvi.10.3.524-536.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burge B. W., Huang A. S. Comparison of membrane protein glycopeptides of Sindbis virus and vesicular stomatitis virus. J Virol. 1970 Aug;6(2):176–182. doi: 10.1128/jvi.6.2.176-182.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grimes W. J., Burge B. W. Modification of Sindbis virus glycoprotein by host-specified glycosyl transferases. J Virol. 1971 Mar;7(3):309–313. doi: 10.1128/jvi.7.3.309-313.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kaluza G., Scholtissek C., Rott R. Inhibition of the multiplication of enveloped RNA-viruses by glucosamine and 2-deoxy-D-glucose. J Gen Virol. 1972 Mar;14(3):251–259. doi: 10.1099/0022-1317-14-3-251. [DOI] [PubMed] [Google Scholar]
  7. Klenk H. D., Scholtissek C., Rott R. Inhibition of glycoprotein biosynthesis of influenza virus by D-glucosamine and 2-deoxy-D-glucose. Virology. 1972 Sep;49(3):723–734. doi: 10.1016/0042-6822(72)90529-6. [DOI] [PubMed] [Google Scholar]
  8. PFEFFERKORN E. R., HUNTER H. S. PURIFICATION AND PARTIAL CHEMICAL ANALYSIS OF SINDBIS VIRUS. Virology. 1963 Jul;20:433–445. doi: 10.1016/0042-6822(63)90092-8. [DOI] [PubMed] [Google Scholar]
  9. Pedersen C. E., Jr, Sagik B. P. Sindbis virus maturation. J Gen Virol. 1973 Mar;18(3):375–379. doi: 10.1099/0022-1317-18-3-375. [DOI] [PubMed] [Google Scholar]
  10. Roth S., McGuire E. J., Roseman S. Evidence for cell-surface glycosyltransferases. Their potential role in cellular recognition. J Cell Biol. 1971 Nov;51(21):536–547. doi: 10.1083/jcb.51.2.536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Scheele C. M., Pfefferkorn E. R. Kinetics of incorporation of structural proteins into Sindbis virions. J Virol. 1969 Apr;3(4):369–375. doi: 10.1128/jvi.3.4.369-375.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schlesinger M. J., Schlesinger S., Burge B. W. Identification of a second glycoprotein in Sindbis virus. Virology. 1972 Feb;47(2):539–541. doi: 10.1016/0042-6822(72)90298-x. [DOI] [PubMed] [Google Scholar]
  13. Schlesinger M. J., Schlesinger S. Large-molecular-weight precursors of sindbis virus proteins. J Virol. 1973 Jun;11(6):1013–1016. doi: 10.1128/jvi.11.6.1013-1016.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schlesinger S., Schlesinger M. J. Formation of Sindbis virus proteins: identification of a precursor for one of the envelope proteins. J Virol. 1972 Nov;10(5):925–932. doi: 10.1128/jvi.10.5.925-932.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sefton B. M., Burge B. W. Biosynthesis of the Sindbis virus carbohydrates. J Virol. 1973 Dec;12(6):1366–1374. doi: 10.1128/jvi.12.6.1366-1374.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Studier F. W. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. doi: 10.1016/0022-2836(73)90003-x. [DOI] [PubMed] [Google Scholar]

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