Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1975 Mar;15(3):458–465. doi: 10.1128/jvi.15.3.458-465.1975

Complementary strands of CELO virus DNA.

A J Robinson, A J Bellett
PMCID: PMC354480  PMID: 1167606

Abstract

When alkali-denatured DNA from CELO virus (an avian adenovirus) was annealed for 15 min at 37 C in 0.1 M NaCl, 70% of the molecules formed single-stranded circles. This is probably due to base pairing of complementary sequences not more than 110 nucleotides long at the ends of the single strands and implies an inverted terminal repetition in the duplex DNA similar to that reported for the DNA from human adenoviruses. The circular molecules had a uniform length that was approximately the same as that of linear single-stranded molecules. The complementary strands of CELO virus DNA were separated on a preparative scale, and at least 40% of the heavy strands and 56% of the light strands were found to be intact as judged by the formation of single-stranded circles.

Full text

PDF
463

Images in this article

460Image
on p.460

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J., Yates V. J., Jasty V., Mancini L. O. In vitro transformation by an avian adenovirus (CELO). I. Hamster-embryo fibroblastic cultures. J Natl Cancer Inst. 1969 Jan;42(1):1–7. [PubMed] [Google Scholar]
  2. Anderson J., Yates V. J., Jasty V., Mancini L. O. The in vitro transformation by an avian adenovirus (CELO). 3. Human amnion cellcultures. J Natl Cancer Inst. 1969 Sep;43(3):575–580. [PubMed] [Google Scholar]
  3. Berns K. I., Kelly T. J., Jr Letter: Visualization of the inverted terminal repetition in adeno-associated virus DNA. J Mol Biol. 1974 Jan 15;82(2):267–271. doi: 10.1016/0022-2836(74)90344-1. [DOI] [PubMed] [Google Scholar]
  4. Garon C. F., Berry K. W., Rose J. A. A unique form of terminal redundancy in adenovirus DNA molecules. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2391–2395. doi: 10.1073/pnas.69.9.2391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gerry H. W., Kelly T. J., Jr, Berns K. I. Arrangement of nucleotide sequences in adeno-associated virus DNA. J Mol Biol. 1973 Sep 15;79(2):207–225. doi: 10.1016/0022-2836(73)90001-6. [DOI] [PubMed] [Google Scholar]
  6. Koczot F. J., Carter B. J., Garon C. F., Rose J. A. Self-complementarity of terminal sequences within plus or minus strands of adenovirus-associated virus DNA. Proc Natl Acad Sci U S A. 1973 Jan;70(1):215–219. doi: 10.1073/pnas.70.1.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Landgraf-Leurs M., Green M. Adenovirus DNA. 3. Separation of the complementary strands of adenovirus types 2, 7 and 12 DNA molecules. J Mol Biol. 1971 Aug 28;60(1):185–202. doi: 10.1016/0022-2836(71)90457-8. [DOI] [PubMed] [Google Scholar]
  8. Laver W. G., Younghusband H. B., Wrigley N. G. Purification and properties of chick embryo lethal orphan virus (an avian adenovirus). Virology. 1971 Sep;45(3):598–614. doi: 10.1016/0042-6822(71)90175-9. [DOI] [PubMed] [Google Scholar]
  9. Rhoades M., MacHattie L. A., Thomas C. A., Jr The P22 bacteriophage DNA molecule. I. The mature form. J Mol Biol. 1968 Oct 14;37(1):21–40. doi: 10.1016/0022-2836(68)90071-5. [DOI] [PubMed] [Google Scholar]
  10. Robinson A. J., Younghusband H. B., Bellett A. J. A circula DNA-protein complex from adenoviruses. Virology. 1973 Nov;56(1):54–69. doi: 10.1016/0042-6822(73)90287-0. [DOI] [PubMed] [Google Scholar]
  11. Sarma P. S., Huebner R. J., Lane W. T. Induction of tumors in hamsters with an avian adenovirus (CELO). Science. 1965 Sep 3;149(3688):1108–1108. doi: 10.1126/science.149.3688.1108. [DOI] [PubMed] [Google Scholar]
  12. Sussenbach J. S., Ellens D. J., Jansz H. S. Studies on the mechanism of replication of adenovirus DNA. II. The nature of single-stranded DNA in replicative intermediates. J Virol. 1973 Nov;12(5):1131–1138. doi: 10.1128/jvi.12.5.1131-1138.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tibbetts C., Pettersson U., Johansson K., Philpson L. Relationship of mRNA from productively infected cells to the complementary strands of adenovirus type 2 DNA. J Virol. 1974 Feb;13(2):370–377. doi: 10.1128/jvi.13.2.370-377.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wolfson J., Dressler D. Adenovirus-2 DNA contains an inverted terminal repetition. Proc Natl Acad Sci U S A. 1972 Oct;69(10):3054–3057. doi: 10.1073/pnas.69.10.3054. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Younghusband H. B., Bellett A. J. Denaturation pattern of the deoxyribonucleic acid from chicken embryo lethal orphan virus. J Virol. 1972 Oct;10(4):855–857. doi: 10.1128/jvi.10.4.855-857.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Younghusband H. B., Bellett A. J. Mature form of the deoxyribonucleic acid from chick embryo lethal orphan virus. J Virol. 1971 Sep;8(3):265–274. doi: 10.1128/jvi.8.3.265-274.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES