Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1975 Apr;15(4):913–917. doi: 10.1128/jvi.15.4.913-917.1975

Inhibition of group B arbovirus antigen production and replication in cells enucleated with cytochalasin B.

K A Kos, B A Osborne, R A Goldsby
PMCID: PMC354536  PMID: 1090752

Abstract

A comparative study of the growth of Sindbis (SIN) virus, a group A arbovirus (togavirus), and Japanese encephalitis (JE) virus, a representative group B arbovirus (togavirus), was conducted in enucleate and nucleate cells. Immunofluorescent tests and yield measurements demonstrated that chicken embryo cells which had been enucleated and subsequently infected with SIN virus produced virus-specific antigens and infectious virus. By contrast, JE failed to replicate or produce virus-specific antigen in cells which had been enucleated before or even 2 h post infection. Studies of the effect of enulceation at various times after infection demonstrated that a nucleus must be present at least 2 and possibly as long as 4 h after infection to produce either JE-specific antigen or infectious JE virus. These studies demonstrate that the replication of SIN, a group A arbovirus (togavirus), which has no nuclear requirement, contrasts sharply with that of a group B arbovirus (togavirus), JE, which may have an initial dependence on a nucleus-associated process.

Full text

PDF
917

Images in this article

915Image
on p.915

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BHAMARAPRAVATI N., HALSTEAD S. B., SOOKAVACHANA P., BOONYAPAKNAVIK V. STUDIES ON DENGUE VIRUS INFECTION. 1. IMMUNOFLUORESCENT LOCALIZATION OF VIRUS IN MOUSE TISSUE. Arch Pathol. 1964 May;77:538–543. [PubMed] [Google Scholar]
  2. BUESCHER E. L., SCHERER W. F., ROSENBERG M. Z., GRESSER I., HARDY J. L., BULLOCK H. R. Ecologic studies of Japanese encephalitis virus in Japan. II. Mosquito infection. Am J Trop Med Hyg. 1959 Nov;8:651–664. doi: 10.4269/ajtmh.1959.8.651. [DOI] [PubMed] [Google Scholar]
  3. Brandt W. E., Buescher E. L., Hetrick F. M. Production and characterization of arbovirus antibody in mouse ascitic fluid. Am J Trop Med Hyg. 1967 May;16(3):339–347. doi: 10.4269/ajtmh.1967.16.339. [DOI] [PubMed] [Google Scholar]
  4. Cardiff R. D., Russ S. B., Brandt W. E., Russell P. K. Cytological localization of Dengue-2 antigens: an immunological study with ultrastructural correlation. Infect Immun. 1973 May;7(5):809–816. doi: 10.1128/iai.7.5.809-816.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eldadah A. H., Nathanson N. Pathogenesis of West Nile Virus encepahlitis in mice and rats. II. Virus multiplication, evolution of immunofluorescence, and development of histological lesions in the brain. Am J Epidemiol. 1967 Nov;86(3):776–790. doi: 10.1093/oxfordjournals.aje.a120786. [DOI] [PubMed] [Google Scholar]
  6. Follett E. A., Pringle C. R., Wunner W. H., Skehel J. J. Virus replication in enucleate cells: vesicular stomatitis virus and influenza virus. J Virol. 1974 Feb;13(2):394–399. doi: 10.1128/jvi.13.2.394-399.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HELLER E. ENHANCEMENT OF CHIKUNGUNYA VIRUS REPLICATION AND INHIBITION OF INTERFERON PRODUCTION BY ACTINOMYCIN D. Virology. 1963 Dec;21:652–656. doi: 10.1016/0042-6822(63)90239-3. [DOI] [PubMed] [Google Scholar]
  8. Matsumura T., Stollar V., Schlesinger R. W. Studies on the nature of dengue viruses. V. Structure and development of dengue virus in Vero cells. Virology. 1971 Nov;46(2):344–355. doi: 10.1016/0042-6822(71)90036-5. [DOI] [PubMed] [Google Scholar]
  9. Murphy F. A., Harrison A. K., Gary G. W., Jr, Whitfield S. G., Forrester F. T. St. Louis encephalitis virus infection in mice. Electron microscopic studies of central nervous system. Lab Invest. 1968 Dec;19(6):652–662. [PubMed] [Google Scholar]
  10. Nishimura C., Tsukeda H. Replication and synthesis of Japanese encephalitis virus ribonucleic acids in Vero cells. Jpn J Microbiol. 1971 Jul;15(4):309–316. doi: 10.1111/j.1348-0421.1971.tb00586.x. [DOI] [PubMed] [Google Scholar]
  11. OTA Z. ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF JAPANESE B ENCEPHALITIS VIRUS IN PORCINE KIDNEY STABLE (PS) CELLS. Virology. 1965 Mar;25:372–378. doi: 10.1016/0042-6822(65)90057-7. [DOI] [PubMed] [Google Scholar]
  12. PFEFFERKORN E. R., HUNTER H. S. PURIFICATION AND PARTIAL CHEMICAL ANALYSIS OF SINDBIS VIRUS. Virology. 1963 Jul;20:433–445. doi: 10.1016/0042-6822(63)90092-8. [DOI] [PubMed] [Google Scholar]
  13. PUCK T. T., CIECIURA S. J., ROBINSON A. Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. J Exp Med. 1958 Dec 1;108(6):945–956. doi: 10.1084/jem.108.6.945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Prescott D. M., Myerson D., Wallace J. Enucleation of mammalian cells with cytochalasin B. Exp Cell Res. 1972;71(2):480–485. doi: 10.1016/0014-4827(72)90322-9. [DOI] [PubMed] [Google Scholar]
  15. Shapiro D., Brandt W. E., Cardiff R. D., Russell P. K. The proteins of Japanese encephalitis virus. Virology. 1971 Apr;44(1):108–124. doi: 10.1016/0042-6822(71)90158-9. [DOI] [PubMed] [Google Scholar]
  16. Stollar V., Stevens T. M., Schlesinger R. W. Studies on the nature of dengue viruses. II. Characterization of viral RNA and effects of inhibitors of RNA synthesis. Virology. 1966 Oct;30(2):303–312. doi: 10.1016/0042-6822(66)90105-x. [DOI] [PubMed] [Google Scholar]
  17. TAKEDA H., YAMADA M. A., AOYAMA Y. DEMONSTRATION OF RNA SYNTHESIS CAUSED BY JAPANESE ENCEPHALITIS VIRUS INFECTION IN PS(Y-15) CELLS WITH THE AID OF CHROMOMYCIN A-3. Jpn J Med Sci Biol. 1965 Apr;18:111–120. doi: 10.7883/yoken1952.18.111. [DOI] [PubMed] [Google Scholar]
  18. TAYLOR R. M., HURLBUT H. S., WORK T. H., KINGSTON J. R., FROTHINGHAM T. E. Sindbis virus: a newly recognized arthropodtransmitted virus. Am J Trop Med Hyg. 1955 Sep;4(5):844–862. doi: 10.4269/ajtmh.1955.4.844. [DOI] [PubMed] [Google Scholar]
  19. Trent D. W., Swensen C. C., Qureshi A. A. Synthesis of Saint Louis encephalitis virus ribonucleic acid in BHK-21-13 cells. J Virol. 1969 Apr;3(4):385–394. doi: 10.1128/jvi.3.4.385-394.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. YASUZUMI G., TSUBO I. ANALYSIS OF THE DEVELOPMENT OF JAPANESE B ENCEPHALITIS (JBE) VIRUS. 3. ELECTRON MICROSCOPE STUDIES ON INCLUSION BODIES APPEARING IN NEURONS AND MICROGLIAL CELLS INFECTED WITH JBE VIRUS. J Ultrastruct Res. 1965 Apr;12:317–327. doi: 10.1016/s0022-5320(65)80102-2. [DOI] [PubMed] [Google Scholar]
  21. YASUZUMI G., TSUBO I. ANALYSIS OF THE DEVELOPMENT OF JAPANESE B ENCEPHALITIS (JBE) VIRUS. II. ELECTRON MICROSCOPE STUDIES OF NEURONS INFECTED WITH JBE VIRUS. J Ultrastruct Res. 1965 Apr;12:304–316. doi: 10.1016/s0022-5320(65)80101-0. [DOI] [PubMed] [Google Scholar]
  22. YASUZUMI G., TSUBO I., SUGIHARA R., NAKAI Y. ANALYSIS OF THE DEVELOPMENT OF JAPANESE B ENCEPHALITIS (JBE) VISUS. I. ELECTRON MICROSCOPE STUDIES OF MICROGLIA INFECTED WITH JBE VIRUS. J Ultrastruct Res. 1964 Oct;11:213–229. doi: 10.1016/s0022-5320(64)90029-2. [DOI] [PubMed] [Google Scholar]
  23. Zebovitz E., Leong J. K., Doughty S. C. Involvement of the host cell nuclear envelope membranes in the replication of Japanese encephalitis virus. Infect Immun. 1974 Jul;10(1):204–211. doi: 10.1128/iai.10.1.204-211.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Zebovitz E., Leong J. K., Doughty S. C. Japanese encephalitis virus replication: a procedure for the selective isolation and characterization of viral RNA species. Arch Gesamte Virusforsch. 1972;38(4):319–327. doi: 10.1007/BF01262822. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES