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. 1975 Apr;15(4):1042–1046. doi: 10.1128/jvi.15.4.1042-1046.1975

Purification and properties of a bacteriophage T5-modified form of Escherichia coli RNA polymerase.

C Szabo, R W Moyer
PMCID: PMC354552  PMID: 1090747

Abstract

A modified form of Escherichia coli RNA polymerase that contains one of the three T5-specific polypeptides known to interact with the host enzyme was purified from bacteriophage T5-infected cells. The properties of this T5-modified enzyme appeared identical to those of the RNA polymerase derived from uninfected non-colicinogenic cells and to a fully active enzyme isolated from T5-infected ColIb+ cells after the limited in vivo transcription of T5 genes allowed by the plasmid had ceased.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burgess R. R. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase. J Biol Chem. 1969 Nov 25;244(22):6160–6167. [PubMed] [Google Scholar]
  2. Cooper T. G. Procedure for uniform binding of DNA to nitrocellulose filters. Anal Biochem. 1972 Jun;47(2):481–486. doi: 10.1016/0003-2697(72)90141-8. [DOI] [PubMed] [Google Scholar]
  3. Lembach K. J., Buchanan J. M. The relationship of protein synthesis to early transcriptive events in bacteriophage T4-infected Escherichia coli B. J Biol Chem. 1970 Apr 10;245(7):1575–1587. [PubMed] [Google Scholar]
  4. Moyer R. W., Buchanan J. M. Effect of calcium ions on synthesis of T5-specific proteins. J Biol Chem. 1970 Nov 25;245(22):5897–5903. [PubMed] [Google Scholar]
  5. Moyer R. W., Buchanan J. M. Patterns of RNA synthesis in T5-infected cells. I. As studied by the technique of DNA-RNA hybridization-competition. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1249–1256. doi: 10.1073/pnas.64.4.1249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Pispa J. P., Buchanan J. M. Synthesis of bacteriophage T5 specific RNA in vitro. Biochim Biophys Acta. 1971 Sep 30;247(1):181–184. doi: 10.1016/0005-2787(71)90823-9. [DOI] [PubMed] [Google Scholar]
  7. Schachner M., Seifert W., Zillig W. A correlation of changes in host and T 4 bacteriophage specific RNA synthesis with changes of DNA-dependent RNA polymerase in Escherichia coli infected with bacteriophage T 4 . Eur J Biochem. 1971 Oct 26;22(4):520–528. doi: 10.1111/j.1432-1033.1971.tb01572.x. [DOI] [PubMed] [Google Scholar]
  8. Stevens A. Deoxyribonucleic acid dependent ribonucleic acid polymerases from two T4 phage-infected systems. Biochemistry. 1974 Jan 29;13(3):493–503. doi: 10.1021/bi00700a015. [DOI] [PubMed] [Google Scholar]
  9. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

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