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. 1975 Aug;16(2):284–289. doi: 10.1128/jvi.16.2.284-289.1975

Interaction of P2 bacteriophage with the dnaB gene of Escherichia coli.

M Sunshine, D Usher, R Calendar
PMCID: PMC354666  PMID: 1097733

Abstract

The dnaB gene product of Escherischia coli is required for multiplication of temperate phage P2. At 37 C in dnaB-ts mutnats, P2 will not plaque and gives a very small burst of progeny. P2 mutants have been isolated which can grow well enough to plaque under these conditions. This type of phage mutant is cis dominant, and one such mutant (P2rlb1) has been mapped near the left end of the early gene B and to the right of the cox4 (excision) mutation. The rlb1 mutation does not lie at the replication origin, but may affect transcription in the early region, which includes the replication origin. It may also represent a site on the P2 DNA which interacts with the dnaB gene product.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERTANI G. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J Bacteriol. 1951 Sep;62(3):293–300. doi: 10.1128/jb.62.3.293-300.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BERTANI L. E. The effect of ultraviolet light on the establishment of lysogeny. Virology. 1959 Jan;7(1):92–111. doi: 10.1016/0042-6822(59)90179-5. [DOI] [PubMed] [Google Scholar]
  3. Bertani G., Bertani L. E. Constitutive expression of bacteriophage P2 early genes resulting from a tandem duplication. Proc Natl Acad Sci U S A. 1974 Feb;71(2):315–319. doi: 10.1073/pnas.71.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bertani G. Deletions in bacteriophage P2. Circularity of the genetic map and its orientation relative to the DNA denaturation map. Mol Gen Genet. 1975;136(2):107–137. doi: 10.1007/BF00272034. [DOI] [PubMed] [Google Scholar]
  5. Bertani L. E. Abortive induction of bacteriophage P2. Virology. 1968 Sep;36(1):87–103. doi: 10.1016/0042-6822(68)90119-0. [DOI] [PubMed] [Google Scholar]
  6. Calendar R., Lindqvist B., Sironi G., Clark A. J. Characterization of REP- mutants and their interaction with P2 phage. Virology. 1970 Jan;40(1):72–83. doi: 10.1016/0042-6822(70)90380-6. [DOI] [PubMed] [Google Scholar]
  7. Chattoraj D. K., Inman R. B. Position of two deletion mutations on the physical map of bacteriophage P2. J Mol Biol. 1972 May 28;66(3):423–434. doi: 10.1016/0022-2836(72)90424-x. [DOI] [PubMed] [Google Scholar]
  8. Chattoraj D. K., Inman R. B. Tandem duplication in bacteriophage P2: electron microscopic mapping. Proc Natl Acad Sci U S A. 1974 Feb;71(2):311–314. doi: 10.1073/pnas.71.2.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Denhardt D. T., Dressler D. H., Hathaway A. THE ABORTIVE REPLICATION OF PhiX174 DNA IN A RECOMBINATION-DEFICIENT MUTANT OF Escherichia coli. Proc Natl Acad Sci U S A. 1967 Mar;57(3):813–820. doi: 10.1073/pnas.57.3.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dumas L. B., Miller C. A. Inhibition of bacteriophage phi chi 174 DNA replication in dnaB mutants of Escherichia coli C. J Virol. 1974 Dec;14(6):1369–1379. doi: 10.1128/jvi.14.6.1369-1379.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fangman W. L., Novick A. Characterization of two bacterial mutants with temperature-sensitive synthesis of DNA. Genetics. 1968 Sep;60(1):1–17. doi: 10.1093/genetics/60.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Geisselsoder J., Mandel M., Calendar R., Chattoraj D. K. In vivo transcription patterns of temperate coliphage P2. J Mol Biol. 1973 Jul 5;77(3):405–415. doi: 10.1016/0022-2836(73)90447-6. [DOI] [PubMed] [Google Scholar]
  13. Kohiyama M., Cousin D., Ryter A., Jacob F. Mutants thermosensibles d'Escherichia coli K 12. I. Isolement et caractérisation rapide. Ann Inst Pasteur (Paris) 1966 Apr;110(4):465–486. [PubMed] [Google Scholar]
  14. Lindahl G. Bacteriophage P2: replication of the chromosome requires a protein which acts only on the genome that coded for it. Virology. 1970 Oct;42(2):522–533. doi: 10.1016/0042-6822(70)90295-3. [DOI] [PubMed] [Google Scholar]
  15. Lindahl G. Genetic map of bacteriophage P2. Virology. 1969 Dec;39(4):839–860. doi: 10.1016/0042-6822(69)90021-x. [DOI] [PubMed] [Google Scholar]
  16. Lindahl G. Multiple recombination mechanisms in bacteriophage P2. Virology. 1969 Dec;39(4):861–866. doi: 10.1016/0042-6822(69)90022-1. [DOI] [PubMed] [Google Scholar]
  17. Lindahl G. On the control of transcription in bacteriophage P2. Virology. 1971 Dec;46(3):620–633. doi: 10.1016/0042-6822(71)90065-1. [DOI] [PubMed] [Google Scholar]
  18. Lindahl G., Sironi G., Bialy H., Calendar R. Bacteriophage lambda; abortive infection of bacteria lysogenic for phage P2. Proc Natl Acad Sci U S A. 1970 Jul;66(3):587–594. doi: 10.1073/pnas.66.3.587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lindahl G., Sunshine M. Excision-deficient mutants of bacteriophage P2. Virology. 1972 Jul;49(1):180–187. doi: 10.1016/s0042-6822(72)80019-9. [DOI] [PubMed] [Google Scholar]
  20. Lindqvist B. H., Bovre K. Asymmetric transcription of the coliphage P2 genome during infection. Virology. 1972 Sep;49(3):690–699. doi: 10.1016/0042-6822(72)90526-0. [DOI] [PubMed] [Google Scholar]
  21. Lindqvist B. H. Vegetative DNA of temperate coliphage P2. Mol Gen Genet. 1971;110(2):178–196. doi: 10.1007/BF00332647. [DOI] [PubMed] [Google Scholar]
  22. Sasaki I., Bertani G. Growth abnormalities in Hfr derivatives of Escherichia coli strain C. J Gen Microbiol. 1965 Sep;40(3):365–376. doi: 10.1099/00221287-40-3-365. [DOI] [PubMed] [Google Scholar]
  23. Schnös M., Inman R. B. Starting point and direction of replication in P2 DNA. J Mol Biol. 1971 Jan 14;55(1):31–38. doi: 10.1016/0022-2836(71)90278-6. [DOI] [PubMed] [Google Scholar]
  24. Six E. W., Klug C. A. Bacteriophage P4: a satellite virus depending on a helper such as prophage P2. Virology. 1973 Feb;51(2):327–344. doi: 10.1016/0042-6822(73)90432-7. [DOI] [PubMed] [Google Scholar]
  25. Sunshine M. G., Thorn M., Gibbs W., Calendar R., Kelly B. P2 phage amber mutants: characterization by use of a polarity suppressor. Virology. 1971 Dec;46(3):691–702. doi: 10.1016/0042-6822(71)90071-7. [DOI] [PubMed] [Google Scholar]
  26. Wiman M., Bertani G., Kelly B., Sasaki I. Genetic map of Escherichia coli strain C. Mol Gen Genet. 1970;107(1):1–31. doi: 10.1007/BF00433220. [DOI] [PubMed] [Google Scholar]

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