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. 1975 Oct;16(4):887–896. doi: 10.1128/jvi.16.4.887-896.1975

Murine intracisternal type A particles: a biochemical characterization.

F Wong-Staal, M S Reitz Jr, C D Trainor, R C Gallo
PMCID: PMC354750  PMID: 51937

Abstract

Intracisternal A particle preparations from a murine neuroblastoma cell line (N18) and from a mineral oil-induced murine plasmacytoma (MOPC-104E) contain both an endogenous RNA-dependent DNA polymerase activity and high molecular-weight polyadenylic acid (poly[A])-containing RNA. The DNA polymerase activity is stimulated by oligo(dG)-poly(C) and oligo(dT)-poly(A) and to a lesser extent by oligo(dT)-poly(dA), in agreement with previous reports. The high-molecular-weight RNA is predominantly 35S and contains a poly(A) tract of approximately 220 nucleotides as judged by polyacrylamide gel electrophoresis. Small amounts of 70S RNA are also present. This RNA preparation contains RNA homologous to RNA from type-C particles, as judged by molecular hybridization experiments. However, since this RNA derives only in part from A-particles and in part from other cellular RNA, hybridization of A-particle endogenously synthesized DNA or reverse transcripts of A-particle RNA to purified type C viral 70S RNA may more accurately reflect the relationship of A-particle RNA to RNA from C-particles. None of these DNA transcripts hybridizes significantly to C-particle 70S RNA, although MOPC and N18 DNA transcripts share significant homology. Our interpretation of these results is that murine intracisternal A particles are not closely related genetically to the tested murine type C viruses, although an alternate possibility is that all the A-particle DNA transcripts are copied from only a small part of the genome, which is unrelated to C-particle RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abrell J. W., Gallo R. C. Purification, characterization, and comparison of the DNA polymerases from two primate RNA tumor viruses. J Virol. 1973 Sep;12(3):431–439. doi: 10.1128/jvi.12.3.431-439.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BERNHARD W. The detection and study of tumor viruses with the electron microscope. Cancer Res. 1960 Jun;20:712–727. [PubMed] [Google Scholar]
  3. Benveniste R. E., Scolnick E. M. RNA in mammalian sarcoma virus transformed nonproducer cells homologous to murine leukemia virus RNA. Virology. 1973 Feb;51(2):370–382. doi: 10.1016/0042-6822(73)90436-4. [DOI] [PubMed] [Google Scholar]
  4. Benveniste R. E., Todaro G. J. Homology between type-C viruses of various species as determined by molecular hybridization. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3316–3320. doi: 10.1073/pnas.70.12.3316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bhattacharyya J., Xuma M., Reitz M., Sarin P. S., Gallo R. C. Utilization of mammalian 70S RNA by a purified reverse transcriptase from human myelocytic leukemic cells. Biochem Biophys Res Commun. 1973 Sep 5;54(1):324–334. doi: 10.1016/0006-291x(73)90926-1. [DOI] [PubMed] [Google Scholar]
  6. Bishop D. H., Claybrook J. R., Spiegelman S. Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967 Jun 28;26(3):373–387. doi: 10.1016/0022-2836(67)90310-5. [DOI] [PubMed] [Google Scholar]
  7. Calarco P. G., Szollosi D. Intracisternal A particles in ova and preimplantation stages of the mouse. Nat New Biol. 1973 May 16;243(124):91–93. [PubMed] [Google Scholar]
  8. DALTON A. J., POTTER M., MERWIN R. M. Some ultrastructural characteristics of a series of primary and transplanted plasma-cell tumors of the mouse. J Natl Cancer Inst. 1961 May;26:1221–1267. [PubMed] [Google Scholar]
  9. Dalton A. J. RNA tumor viruses. Terminology and ultrastructural aspects of virion morphology and replication. J Natl Cancer Inst. 1972 Aug;49(2):323–327. [PubMed] [Google Scholar]
  10. Dion A. S., Vaidya B., Fout G. S., Moore D. H. Isolation and characterization of RNA-directed DNA polymerase from a B-type RNA tumor virus. J Virol. 1974 Jul;14(1):40–46. doi: 10.1128/jvi.14.1.40-46.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Garapin A. C., Varmus H. E., Faras A. J., Levinson W. E., Bishop J. M. RNA-directed DNA synthesis by virions of Rous sarcoma virus: further characterization of the templates and the extent of their transcription. Virology. 1973 Mar;52(1):264–274. doi: 10.1016/0042-6822(73)90414-5. [DOI] [PubMed] [Google Scholar]
  12. Gardner M. B., Officer J. E., Rongey R. W., Estes J. D., Turner H. C., Huebner R. J. C-type RNA tumour virus genome expression in wild house mice. Nature. 1971 Aug 27;232(5313):617–620. doi: 10.1038/232617a0. [DOI] [PubMed] [Google Scholar]
  13. Hall W. T., Hartley J. W., Sanford K. K. Characteristics of and relationship between C particles and intracisternal A particles in cloned cell strains. J Virol. 1968 Mar;2(3):238–247. doi: 10.1128/jvi.2.3.238-247.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ihle J. N., Lee K. L., Kennedy F. T. Fractionation of 34 S ribonucleic acid subunits from oncornaviruses on polyuridylate-sepharose columns. J Biol Chem. 1974 Jan 10;249(1):38–42. [PubMed] [Google Scholar]
  15. Kuff E. L., Leuders K. K., Ozer H. L., Wivel N. A. Some structural and antigenic properties of intracisternal A particles occurring in mouse tumors (complement fixation-immunodiffusion-neuroblastoma-plasma-cell tumor). Proc Natl Acad Sci U S A. 1972 Jan;69(1):218–222. doi: 10.1073/pnas.69.1.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kuff E. L., Wivel N. A., Lueders K. K. The extraction of intracisternal A-particles from a mouse plasma-cell tumor. Cancer Res. 1968 Oct;28(10):2137–2148. [PubMed] [Google Scholar]
  17. Miller N. R., Saxinger W. C., Reitz M. S., Gallagher R. E., Wu A. M., Gallo R. C., Gillespie D. Systematics of RNA tumor viruses and virus-like particles of human origin. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3177–3181. doi: 10.1073/pnas.71.8.3177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Reitz M. S., Jr, Abrell J. W., Trainor C. D., Gallo R. C. Precipitation of nucleic acids with cetyltrimethylammonium bromide: a method for preparing viral and cellular DNA polymerase products for cesium sulfate density gradient analysis. Biochem Biophys Res Commun. 1972 Oct 6;49(1):30–38. doi: 10.1016/0006-291x(72)90005-8. [DOI] [PubMed] [Google Scholar]
  19. Robert M. S., Smith R. G., Gallo R. C., Sarin P. S., Abrell J. W. Viral and cellular DNA polymerase: comparison of activities with synthetic and natural RNA templates. Science. 1972 May 19;176(4036):798–800. doi: 10.1126/science.176.4036.798. [DOI] [PubMed] [Google Scholar]
  20. Robertson D. L., Baenziger N. L., Dobbertin D. C., Thach R. E. Characterization of DNA polymerase and RNA associated with A-type particles from murine myeloma cells. J Virol. 1975 Feb;15(2):407–415. doi: 10.1128/jvi.15.2.407-415.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Swan D., Aviv H., Leder P. Purification and properties of biologically active messenger RNA for a myeloma light chain. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1967–1971. doi: 10.1073/pnas.69.7.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tavitian A., Hamelin R., Tchen P., Olofsson B., Boiron M. Extent of transcription of mouse sarcoma-leukemia virus by RNA-directed DNA polymerase. Proc Natl Acad Sci U S A. 1974 Mar;71(3):755–759. doi: 10.1073/pnas.71.3.755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tsuchida N., Robin M. S., Green M. Viral RNA subunits in cells transformed by RNA tumor viruses. Science. 1972 Jun 30;176(4042):1418–1420. doi: 10.1126/science.176.4042.1418. [DOI] [PubMed] [Google Scholar]
  24. Wilson S. H., Bohn E. W., Matsukage A., Lueders K. K., Kuff E. L. Studies on the relationship between deoxyribonucleic acid polymerase activity and intracisternal A-type particles in mouse myeloma. Biochemistry. 1974 Mar 12;13(6):1087–1094. doi: 10.1021/bi00703a005. [DOI] [PubMed] [Google Scholar]
  25. Wilson S. H., Kuff E. L. A novel DNA polymerase activity found in association with intracisternal A-type particles. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1531–1536. doi: 10.1073/pnas.69.6.1531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wu A. M., Reitz M. S., Paran M., Gallo R. C. Mechanism of stimulation of murine type-C RNA tumor virus production by glucocorticoids: post-transcriptional effects. J Virol. 1974 Oct;14(4):802–812. doi: 10.1128/jvi.14.4.802-812.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Yang S. S., Wivel N. A. Analysis of high-molecular-weight ribonucleic acid associated with intracisternal A particles. J Virol. 1973 Feb;11(2):287–298. doi: 10.1128/jvi.11.2.287-298.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Yang S. S., Wivel N. A. Characterization of an endogenous RNA-dependent DNA polymerase associated with murine intracisternal A particles. J Virol. 1974 Mar;13(3):712–720. doi: 10.1128/jvi.13.3.712-720.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Yotsuyanagi Y., Ephrussi B. Behavior of three types of ribovirus-like particles in segregating hamster times mouse somatic hybrids. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4575–4578. doi: 10.1073/pnas.71.11.4575. [DOI] [PMC free article] [PubMed] [Google Scholar]

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