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. 1976 Jun;18(3):873–884. doi: 10.1128/jvi.18.3.873-884.1976

Absence of interparental recombination in multiplicity reconstitution from incomplete bacteriophage T4 genomes.

A W Kozinski, A H Doermann, P B Kozinski
PMCID: PMC354786  PMID: 1271533

Abstract

Interparental recombination between injected T4 DNA molecules is indetectable for incomplete petite phages (carrying a terminally deficient genome and therefore unable to circularize) as well as for genetically complete phages. The nonvialbe petite phages can individually replicate their DNA repeatedly, and they aso undergo multiplicity reconstitution, producing complete phages, provided that a host bacterium is infected by several petite particles that carry genetically complementary segments of DNA. The formation of complete phages in multiplicity reconstitution must be due to recombination among incomplete progeny fragments, i.e., partial replicas of the T4 genomes. It evidently does not result from interparental recombination. To test for interparental recombination, light bacteria (containing no bromouracil) were simultaneously infected in light medium with light radioactive phage in minority (usually less than one per cell) and heavy (bromouracil-labeled) phage in majority (usually about nine per cell). Any interparental recombination should, under these circumstances of infection, head to movement of the radioactive label of the minority light phage DNA to a position of higher density. That possibility was not observed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANRAKU N., TOMIZAWA J. MOLECULAR MECHANISMS OF GENETIC RECOMBINATION IN BACTERIOPHAGE. 3. JOINING OF PARENTAL POLYNUCLEOTIDES OF PHAGE T4 IN THE PRESENCE OF 5-FLUORODEOXYURIDINE. J Mol Biol. 1965 Mar;11:501–508. doi: 10.1016/s0022-2836(65)80006-7. [DOI] [PubMed] [Google Scholar]
  2. Broker T. R., Doermann A. H. Molecular and genetic recombination of bacteriophage T4. Annu Rev Genet. 1975;9:213–244. doi: 10.1146/annurev.ge.09.120175.001241. [DOI] [PubMed] [Google Scholar]
  3. Delius H., Howe C., Kozinski A. W. Structure of the replicating DNA from bacteriophage T4. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3049–3053. doi: 10.1073/pnas.68.12.3049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Doermann A. H., Eiserling F. A., Boehner L. Genetic control of capsid length in bacteriophage T4. I. Isolation and preliminary description of four new mutants. J Virol. 1973 Aug;12(2):374–385. doi: 10.1128/jvi.12.2.374-385.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doermann A. H., Parma D. H. Recombination in bacteriophage T4. J Cell Physiol. 1967 Oct;70(2 Suppl):147–164. doi: 10.1002/jcp.1040700411. [DOI] [PubMed] [Google Scholar]
  6. Doermann A. H. T4 and the rolling circle model of replication. Annu Rev Genet. 1973;7:325–341. doi: 10.1146/annurev.ge.07.120173.001545. [DOI] [PubMed] [Google Scholar]
  7. EPSTEIN R. H. A study of multiplicity-reactivation in the bacteriophage T4. I. Genetic and functional analysis of T4D-K12(lambda) complexes. Virology. 1958 Oct;6(2):382–404. doi: 10.1016/0042-6822(58)90090-4. [DOI] [PubMed] [Google Scholar]
  8. Howe C. C., Buckley P. J., Carlson K. M., Kozinski A. W. Multiple and specific initiation of T4 DNA replication. J Virol. 1973 Jul;12(1):130–148. doi: 10.1128/jvi.12.1.130-148.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KOZINSKI A. W., KOZINSKI P. B. REPLICATIVE FRAGMENTATION IN T4 BACTERIOPHAGE DNA. II. BIPARENTAL MOLECULAR RECOMBINATION. Proc Natl Acad Sci U S A. 1964 Aug;52:211–218. doi: 10.1073/pnas.52.2.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KOZINSKI A. W., SZYBALSKI W. Dispersive transfer of the parental DNA molecule to the progeny of phage phiX-174. Virology. 1959 Oct;9:260–274. doi: 10.1016/0042-6822(59)90119-9. [DOI] [PubMed] [Google Scholar]
  11. Kozinski A. W., Doermann A. H. Repetitive DNA replication of the incomplete genomes of phage T4 petite particles. Proc Natl Acad Sci U S A. 1975 May;72(5):1734–1738. doi: 10.1073/pnas.72.5.1734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kozinski A. W., Felgenhauer Z. Z. Molecular recombination in T4 bacteriophage deoxyribonucleic acid. II. Single-strand breaks and exposure of uncomplemented areas as a prerequisite for recombination. J Virol. 1967 Dec;1(6):1193–1202. doi: 10.1128/jvi.1.6.1193-1202.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kozinski A. W., Kozinski P. B. Autonomous replication of short DNA fragments in the ligase negative T4 AM H39X. Biochem Biophys Res Commun. 1968 Nov 25;33(4):670–674. doi: 10.1016/0006-291x(68)90348-3. [DOI] [PubMed] [Google Scholar]
  14. Kozinski A. W., Kozinski P. B., James R. Molecular recombination in T4 bacteriophage deoxyribonucleic acid. I. Tertiary structure of early replicative and recombining deoxyribonucleic acid. J Virol. 1967 Aug;1(4):758–770. doi: 10.1128/jvi.1.4.758-770.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kozinski A. W., Kozinski P. B., Shannon P. REPLICATIVE FRAGMENTATION IN T4 PHAGE: INHIBITION BY CHLORAMPHENICOL. Proc Natl Acad Sci U S A. 1963 Oct;50(4):746–753. doi: 10.1073/pnas.50.4.746. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kozinski A. W., Lin T. H. Early intracellular events in the replication of T4 phage DNA. I. Complex formation of replicative DNA. Proc Natl Acad Sci U S A. 1965 Jul;54(1):273–278. doi: 10.1073/pnas.54.1.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kozinski A. W., Mitchell M. Restoration by Chloramphenicol of Bacteriophage Production in Escherichia coli B Infected with a Ligase-Deficient Amber Mutant. J Virol. 1969 Dec;4(6):823–836. doi: 10.1128/jvi.4.6.823-836.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kozinski A. W. Molecular recombination in the ligase negative T4 amber mutant. Cold Spring Harb Symp Quant Biol. 1968;33:375–391. doi: 10.1101/sqb.1968.033.01.044. [DOI] [PubMed] [Google Scholar]
  19. Luria S. E. Reactivation of Irradiated Bacteriophage by Transfer of Self-Reproducing Units. Proc Natl Acad Sci U S A. 1947 Sep;33(9):253–264. doi: 10.1073/pnas.33.9.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mosig G., Ehring R., Schliewen W., Bock S. The patterns of recombination and segregation in terminal regions of T4DNA molecules. Mol Gen Genet. 1971;113(1):51–91. doi: 10.1007/BF00335007. [DOI] [PubMed] [Google Scholar]
  21. Parma D. H. The structure of genomes of individual petit particles of the bacteriophage T4D mutant E920/96/41. Genetics. 1969 Oct;63(2):247–261. doi: 10.1093/genetics/63.2.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. STAHL F. W., EDGAR R. S., STEINBERG J. THE LINKAGE MAP OF BACTERIOPHAGE T4. Genetics. 1964 Oct;50:539–552. doi: 10.1093/genetics/50.4.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tomizawa J. I. Molecular mechanisms of genetic recombination in bacteriophage: joint molecules and their conversion to recombinant molecules. J Cell Physiol. 1967 Oct;70(2 Suppl):201–213. doi: 10.1002/jcp.1040700414. [DOI] [PubMed] [Google Scholar]
  24. Womack F. C. Cross-reactivation differences in bacteriophage T4D. Virology. 1965 Aug;26(4):758–760. [PubMed] [Google Scholar]

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