Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1976 Jun;18(3):885–893. doi: 10.1128/jvi.18.3.885-893.1976

Herpes simplex virus DNA in transformed cells: sequence complexity in five hamster cell lines and one derived hamster tumor.

N Frenkel, H Locker, B Cox, B Roizman, F Rapp
PMCID: PMC354787  PMID: 178927

Abstract

Analyses of the hybridization kinetics of labeled herpes simplex virus 2 (HSV-2) DNA with DNA from five hamster cell lines transformed by UV light-irradiated HSV-2 revealed the following. (i) Viral DNA sequences were detected in all five cell lines tested. (ii) None of the cell lines contained the full complement of HSV-2 DNA. (iii) The amount of viral DNA present in the cells varied in different transformed cell lines and ranged from 8 to 32% of the HSV-2 DNA genome in 1 to 3 copies/cell. (iv) Two parallel passages of the same cell line (333-2-29) differed in the amount of viral DNA they contained. We also compared the viral DNA sequences present in (i) one transformed cell line (333-8-9) propagated serially in culture for 80 passages, (ii) a tumor produced by inoculation of a newborn hamster with the 333-8-9 cells, and (iii) a cell line derived from a hamster tumor as above and propagated in culture for 32 passages. The results show that viral DNA present in the hamster tumor and in the cells derived from the tumor had a lower sequence complexity than that present in the original serially passaged 333-8-9 cell line.

Full text

PDF
893

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ben-Porat T., Demarchi J. M., Kaplan A. S. Characterization of defective interfering viral particles present in a population of pseudorabies virions. Virology. 1974 Sep;61(1):29–37. doi: 10.1016/0042-6822(74)90239-6. [DOI] [PubMed] [Google Scholar]
  2. Botchan M., Ozanne B., Sugden B., Sharp P. A., Sambrook J. Viral DNA in transformed cells. III. The amounts of different regions of the SV40 genome present in a line of transformed mouse cells. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4183–4187. doi: 10.1073/pnas.71.10.4183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
  4. Bronson D. L., Dreesman G. R., Biswal N., Benyesh-Melnick M. Defective virions of herpes simplex viruses. Intervirology. 1973;1(3):141–153. doi: 10.1159/000148841. [DOI] [PubMed] [Google Scholar]
  5. Collard W., Thornton H., Green M. Cells transformed by human Herpesvirus type 2 transcribe virus-specific RNA sequences shared by Herpesvirus types 1 and 2. Nat New Biol. 1973 Jun 27;243(130):264–266. doi: 10.1038/newbio243264a0. [DOI] [PubMed] [Google Scholar]
  6. Davidson R. L., Adelstein S. J., Oxman M. N. Herpes simplex virus as a source of thymidine kinase for thymidine kinase-deficient mouse cells: suppression and reactivation of the viral enzyme. Proc Natl Acad Sci U S A. 1973 Jul;70(7):1912–1916. doi: 10.1073/pnas.70.7.1912. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Duff R., Kreider J. W., Levy B. M., Katz M., Rapp F. Comparative pathology of cells transformed by herpes simplex virus type 1 or type 2. J Natl Cancer Inst. 1974 Oct;53(4):1159–1164. doi: 10.1093/jnci/53.4.1159. [DOI] [PubMed] [Google Scholar]
  8. Duff R., Rapp F. Oncogenic transformation of hamster embryo cells after exposure to inactivated herpes simplex virus type 1. J Virol. 1973 Aug;12(2):209–217. doi: 10.1128/jvi.12.2.209-217.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Duff R., Rapp F. Properties of hamster embryo fibroblasts transformed in vitro after exposure to ultraviolet-irradiated herpes simplex virus type 2. J Virol. 1971 Oct;8(4):469–477. doi: 10.1128/jvi.8.4.469-477.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Frenkel N., Jacob R. J., Honess R. W., Hayward G. S., Locker H., Roizman B. Anatomy of herpes simplex virus DNA. III. Characterization of defective DNA molecules and biological properties of virus populations containing them. J Virol. 1975 Jul;16(1):153–167. doi: 10.1128/jvi.16.1.153-167.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Frenkel N., Lavi S., Winocour E. The host DNA sequences in different populations of serially passaged SV40. Virology. 1974 Jul;60(1):9–20. doi: 10.1016/0042-6822(74)90360-2. [DOI] [PubMed] [Google Scholar]
  12. Frenkel N., Roizman B., Cassai E., Nahmias A. A DNA fragment of Herpes simplex 2 and its transcription in human cervical cancer tissue. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3784–3789. doi: 10.1073/pnas.69.12.3784. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gallimore P. H. Viral DNA in transformed cells. II. A study of the sequences of adenovirus 2 DNA IN NINE LINES OF TRANSFORMED RAT CELLS USING SPECIFIC FRAGMENTS OF THE VIRAL GENOME;. J Mol Biol. 1974 Oct 15;89(1):49–72. doi: 10.1016/0022-2836(74)90162-4. [DOI] [PubMed] [Google Scholar]
  14. Graham F. L., Abrahams P. J., Mulder C., Heijneker H. L., Warnaar S. O., De Vries F. A., Fiers W., Van Der Eb A. J. Studies on in vitro transformation by DNA and DNA fragments of human adenoviruses and simian virus 40. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):637–650. doi: 10.1101/sqb.1974.039.01.077. [DOI] [PubMed] [Google Scholar]
  15. Hayward G. S., Frenkel N., Roizman B. Anatomy of herpes simplex virus DNA: strain differences and heterogeneity in the locations of restriction endonuclease cleavage sites. Proc Natl Acad Sci U S A. 1975 May;72(5):1768–1772. doi: 10.1073/pnas.72.5.1768. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kieff E. D., Bachenheimer S. L., Roizman B. Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. J Virol. 1971 Aug;8(2):125–132. doi: 10.1128/jvi.8.2.125-132.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LIN H. J., CHARGAFF E. METAPHASE CHROMOSOMES AS A SOURCE OF DNA. Biochim Biophys Acta. 1964 Dec 16;91:691–694. doi: 10.1016/0926-6550(64)90032-5. [DOI] [PubMed] [Google Scholar]
  18. Lee T. N., Nathans D. A transcriptional map of the SV40 genome in transformed cell lines. Virology. 1975 Jan;63(1):263–272. doi: 10.1016/0042-6822(75)90390-6. [DOI] [PubMed] [Google Scholar]
  19. Munyon W., Kraiselburd E., Davis D., Mann J. Transfer of thymidine kinase to thymidine kinaseless L cells by infection with ultraviolet-irradiated herpes simplex virus. J Virol. 1971 Jun;7(6):813–820. doi: 10.1128/jvi.7.6.813-820.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nonoyama M., Pagano J. S. Homology between Epstein-Barr virus DNA and viral DNA from Burkitt's lymphoma and nasopharyngeal carcinoma determined by DNA-DNA reassociation kinetics. Nature. 1973 Mar 2;242(5392):44–47. doi: 10.1038/242044a0. [DOI] [PubMed] [Google Scholar]
  21. Ozanne B., Sharp P. A., Sambrook J. Transcription of simian virus 40. II. Hybridization of RNA extracted from different lines of transformed cells to the separated strands of simian virus 40 DNA. J Virol. 1973 Jul;12(1):90–98. doi: 10.1128/jvi.12.1.90-98.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. ROIZMAN B. Polykaryocytosis. Cold Spring Harb Symp Quant Biol. 1962;27:327–342. doi: 10.1101/sqb.1962.027.001.031. [DOI] [PubMed] [Google Scholar]
  23. Rapp F., Duff R. Transformation of hamster embryo fibroblasts by herpes simplex viruses type 1 and type 2. Cancer Res. 1973 Jun;33(6):1527–1534. [PubMed] [Google Scholar]
  24. Rapp F. Herpesviruses and cancer. Adv Cancer Res. 1974;19(0):265–302. doi: 10.1016/s0065-230x(08)60056-1. [DOI] [PubMed] [Google Scholar]
  25. Roizman B., Spear P. G. Herpesvirus antigens on cell membranes detected by centrifugation of membrane-antibody complexes. Science. 1971 Jan 22;171(3968):298–300. doi: 10.1126/science.171.3968.298. [DOI] [PubMed] [Google Scholar]
  26. Sambrook J., Botchan M., Gallimore P., Ozanne B., Pettersson U., Williams J., Sharp P. A. Viral DNA sequences in cells transformed by simian virus 40, adenovirus type 2 and adenovirus type 5. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):615–632. doi: 10.1101/sqb.1974.039.01.075. [DOI] [PubMed] [Google Scholar]
  27. Sambrook J., Sharp P. A., Keller W. Transcription of Simian virus 40. I. Separation of the strands of SV40 DNA and hybridization of the separated strands to RNA extracted from lytically infected and transformed cells. J Mol Biol. 1972 Sep 14;70(1):57–71. doi: 10.1016/0022-2836(72)90163-5. [DOI] [PubMed] [Google Scholar]
  28. Sharp P. A., Pettersson U., Sambrook J. Viral DNA in transformed cells. I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome. J Mol Biol. 1974 Jul 15;86(4):709–726. doi: 10.1016/0022-2836(74)90348-9. [DOI] [PubMed] [Google Scholar]
  29. Sutton W. D. A crude nuclease preparation suitable for use in DNA reassociation experiments. Biochim Biophys Acta. 1971 Jul 29;240(4):522–531. doi: 10.1016/0005-2787(71)90709-x. [DOI] [PubMed] [Google Scholar]
  30. Takahashi M., Yamanishi K. Transformation of hamster embryo and human embryo cells by temperature sensitive mutants of herpes simplex virus type 2. Virology. 1974 Sep;61(1):306–311. doi: 10.1016/0042-6822(74)90267-0. [DOI] [PubMed] [Google Scholar]
  31. Thuring R. W., Sanders J. P., Borst P. A freeze-squeeze method for recovering long DNA from agarose gels. Anal Biochem. 1975 May 26;66(1):213–220. doi: 10.1016/0003-2697(75)90739-3. [DOI] [PubMed] [Google Scholar]
  32. Wadsworth S., Hayward G. S., Roizman B. Anatomy of herpes simplex virus DNA. V. Terminally repetitive sequences. J Virol. 1976 Feb;17(2):503–512. doi: 10.1128/jvi.17.2.503-512.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Wadsworth S., Jacob R. J., Roizman B. Anatomy of herpes simplex virus DNA. II. Size, composition, and arrangement of inverted terminal repetitions. J Virol. 1975 Jun;15(6):1487–1497. doi: 10.1128/jvi.15.6.1487-1497.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Wagner M., Skare J., Summers W. C. Analysis of DNA of defective herpes simplex virus type 1 by restriction endonuclease cleavage and nucleic acid hybridization. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):683–686. doi: 10.1101/sqb.1974.039.01.082. [DOI] [PubMed] [Google Scholar]
  35. zur Hausen H. Epstein-Barr virus in human tumor cells. Int Rev Exp Pathol. 1972;11:233–258. [PubMed] [Google Scholar]
  36. zur Hausen H., Schulte-Holthausen H., Wolf H., Dörries K., Egger H. Attempts to detect virus-specific DNA in human tumors. II. Nucleic acid hybridizations with complementary RNA of human herpes group viruses. Int J Cancer. 1974 May 15;13(5):657–664. doi: 10.1002/ijc.2910130510. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES