Abstract
Simian virus 40-transformed hamster cells were induced to produce infectious virus by treatment with mitomycin C or gamma-irradiation. A portion of the simian virus-40 DNA, which is integrated into the host cell genome in uninduced cells, was recovered in a pool of relatively low-molecular-weight DNA early after induction treatment in the absence of DNA replication. The data indicte that excision of the viral genome occurs subsequent to the induction stimulus.
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Selected References
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- Black P. H. The oncogenic DNA viruses: a review of in vitro transformation studies. Annu Rev Microbiol. 1968;22:391–426. doi: 10.1146/annurev.mi.22.100168.002135. [DOI] [PubMed] [Google Scholar]
- Gelb L. D., Kohne D. E., Martin M. A. Quantitation of Simian virus 40 sequences in African green monkey, mouse and virus-transformed cell genomes. J Mol Biol. 1971 Apr 14;57(1):129–145. doi: 10.1016/0022-2836(71)90123-9. [DOI] [PubMed] [Google Scholar]
- Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
- Kaplan J. C., Kleinman L. F., Black P. H. Cell cycle dependence of simian virus 40 induction from transformed hamster cells by ultraviolet irradiation. Virology. 1975 Nov;68(1):215–220. doi: 10.1016/0042-6822(75)90162-2. [DOI] [PubMed] [Google Scholar]
- Manor H., Fogel M., Sachs L. Integration of viral into chromosomal deoxyribonucleic acid in an inducible line of polyoma-transformed cells. Virology. 1973 May;53(1):174–185. doi: 10.1016/0042-6822(73)90476-5. [DOI] [PubMed] [Google Scholar]
- Ozanne B., Sharp P. A., Sambrook J. Transcription of simian virus 40. II. Hybridization of RNA extracted from different lines of transformed cells to the separated strands of simian virus 40 DNA. J Virol. 1973 Jul;12(1):90–98. doi: 10.1128/jvi.12.1.90-98.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sambrook J., Westphal H., Srinivasan P. R., Dulbecco R. The integrated state of viral DNA in SV40-transformed cells. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1288–1295. doi: 10.1073/pnas.60.4.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Signer E. R. Lysogeny: the integration problem. Annu Rev Microbiol. 1968;22:451–488. doi: 10.1146/annurev.mi.22.100168.002315. [DOI] [PubMed] [Google Scholar]
- Wetmur J. G., Davidson N. Kinetics of renaturation of DNA. J Mol Biol. 1968 Feb 14;31(3):349–370. doi: 10.1016/0022-2836(68)90414-2. [DOI] [PubMed] [Google Scholar]