Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1976 Aug;19(2):318–324. doi: 10.1128/jvi.19.2.318-324.1976

Action of ethyl and methyl methane sulfonates on DNA injection and genetic recombination in T7 bacteriophage.

B Karska-Wysocki, M D Mamet-Bratley, W G Verly
PMCID: PMC354868  PMID: 183007

Abstract

After treatment with methyl or ethyl methane sulfonate, T7 amber mutants display a reduced capacity for recombination. Moreover, alkylation reduces recombination frequency involving markers on the right-hand side of the genetic map more than it reduces recombination frequency involving markers on the left-hand side. We interpret this to mean that alkylation can stop DNA injection at any point along the DNA molecule, and that T7 phage injects its DNA in a unique fashion starting from the end carrying the genes for early proteins.

Full text

PDF
324

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brakier L., Verly W. G. The lethal action of ehtyl methanesulfonate, nitrogen mustard and myleran on the T7 coliphage. Biochim Biophys Acta. 1970 Aug 8;213(2):296–311. doi: 10.1016/0005-2787(70)90038-9. [DOI] [PubMed] [Google Scholar]
  2. Doermann A. H., Boehner L. The identification of complex genotypes in bacteriophage T4. I. Methods. Genetics. 1970 Nov;66(3):417–428. doi: 10.1093/genetics/66.3.417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Doermann A. H. T4 and the rolling circle model of replication. Annu Rev Genet. 1973;7:325–341. doi: 10.1146/annurev.ge.07.120173.001545. [DOI] [PubMed] [Google Scholar]
  4. Dressler D., Wolfson J., Magazin M. Initiation and reinitiation of DNA synthesis during replication of bacteriophage T7. Proc Natl Acad Sci U S A. 1972 Apr;69(4):998–1002. doi: 10.1073/pnas.69.4.998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dussault P., Bourgault J. M., Verly W. G. T7 coliphage inactivation by nitrous acid. Biochim Biophys Acta. 1970 Aug 8;213(2):312–319. doi: 10.1016/0005-2787(70)90039-0. [DOI] [PubMed] [Google Scholar]
  6. Golomb M., Chamberlin M. A preliminary map of the major transcription units read by T7 RNA polymerase on the T7 and T3 bacteriophage chromosomes. Proc Natl Acad Sci U S A. 1974 Mar;71(3):760–764. doi: 10.1073/pnas.71.3.760. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hausmann R., Gomez B. Amber mutants of bacteriophages T3 and T7 defective in phage-directed deoxyribonucleic acid synthesis. J Virol. 1967 Aug;1(4):779–792. doi: 10.1128/jvi.1.4.779-792.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hyman R. W., Brunovskis I., Summers W. C. DNA base sequence homology between coliphages T7 and phiII and between T3 and phiII as determined by heteroduplex mapping in the electron microscope. J Mol Biol. 1973 Jun 25;77(2):189–196. doi: 10.1016/0022-2836(73)90330-6. [DOI] [PubMed] [Google Scholar]
  9. Kerr C., Sadowski P. D. The involvement of genes 3,4,5 and 6 in genetic recombination in bacteriophage T7. Virology. 1975 May;65(1):281–285. doi: 10.1016/0042-6822(75)90031-8. [DOI] [PubMed] [Google Scholar]
  10. Krisch R. E. Lethal and genetic effects of radiophosphorus decay in bacteriophages SP82G and T7. Int J Radiat Biol Relat Stud Phys Chem Med. 1974 Mar;25(3):261–276. doi: 10.1080/09553007414550321. [DOI] [PubMed] [Google Scholar]
  11. Lawley P. D., Lethbridge J. H., Edwards P. A., Shooter K. V. Inactivation of bacteriophage T7 by mono- and difunctional sulphur mustards in relation to cross-linking and depurination of bacteriophage DNA. J Mol Biol. 1969 Jan 14;39(1):181–198. doi: 10.1016/0022-2836(69)90341-6. [DOI] [PubMed] [Google Scholar]
  12. Pao C. C., Speyer J. F. Order of injection of T7 bacteriophage DNA. J Virol. 1973 Jun;11(6):1024–1026. doi: 10.1128/jvi.11.6.1024-1026.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Saigo K. Polar DNA ejection in bacteriophage T7. Virology. 1975 May;65(1):120–127. doi: 10.1016/0042-6822(75)90012-4. [DOI] [PubMed] [Google Scholar]
  14. Simon M. N., Studier F. W. Physical mapping of the early region of bacteriophage T7 DNA. J Mol Biol. 1973 Sep 15;79(2):249–265. doi: 10.1016/0022-2836(73)90004-1. [DOI] [PubMed] [Google Scholar]
  15. Studier F. W. Bacteriophage T7. Science. 1972 Apr 28;176(4033):367–376. doi: 10.1126/science.176.4033.367. [DOI] [PubMed] [Google Scholar]
  16. Studier F. W. The genetics and physiology of bacteriophage T7. Virology. 1969 Nov;39(3):562–574. doi: 10.1016/0042-6822(69)90104-4. [DOI] [PubMed] [Google Scholar]
  17. Verly W. G., Crine P., Bannon P., Forget A. Immediate inactivation of T7 coliphage treated by monofunctional alkylating agents. Biochim Biophys Acta. 1974 May 17;349(2):204–213. doi: 10.1016/0005-2787(74)90081-1. [DOI] [PubMed] [Google Scholar]
  18. Womack F. C. Cross-reactivation differences in bacteriophage T4D. Virology. 1965 Aug;26(4):758–760. [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES