Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1976 Aug;19(2):338–345. doi: 10.1128/jvi.19.2.338-345.1976

Enzymatic degradation of uracil-containing DNA. II. Evidence for N-glycosidase and nuclease activities in unfractionated extracts of Bacillus subtilis.

J Duncan, L Hamilton, E C Friedberg
PMCID: PMC354871  PMID: 822172

Abstract

Further studies have confirmed our earlier observations that in the presence of EDTA, degradation of phage PBS2 [3H]uracil-labeled DNA is effected by an N-glycosidase activity in extracts of Bacillus subtilis that removes free uracil from DNA. In addition, such extracts contain a nuclease activity that attacks PBS2 DNA in the presence of CaCl2. The nuclease activity is not observed under conditions that inactivate N-glycosidase activity but does attack DNA that has been preincubated to remove uracil by N-glycosidase action. We therefore postulate that the nuclease requires N-glycosidase action to generate substrate for its activity, i.e., the nuclease appears to attack depyrimidinated sites rather than uracil sites in phage PBS2 DNA.

Full text

PDF
345

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chang L. M., Bollum F. J. Deoxynucleotide-polymerizing enzymes of calf thymus gland. V. Homogeneous terminal deoxynucleotidyl transferase. J Biol Chem. 1971 Feb 25;246(4):909–916. [PubMed] [Google Scholar]
  2. Friedberg E. C., Ganesan A. K., Minton K. N-Glycosidase activity in extracts of Bacillus subtilis and its inhibition after infection with bacteriophage PBS2. J Virol. 1975 Aug;16(2):315–321. doi: 10.1128/jvi.16.2.315-321.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Friedberg E. C., Goldthwait D. A. Endonuclease II of E. coli. I. Isolation and purification. Proc Natl Acad Sci U S A. 1969 Mar;62(3):934–940. doi: 10.1073/pnas.62.3.934. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hunter B. I., Yamagishi H., Takahashi I. Molecular weight of bacteriophage PBS 1 deoxyribonucleic acid. J Virol. 1967 Aug;1(4):841–842. doi: 10.1128/jvi.1.4.841-842.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lindahl T. An N-glycosidase from Escherichia coli that releases free uracil from DNA containing deaminated cytosine residues. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3649–3653. doi: 10.1073/pnas.71.9.3649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lindahl T. New class of enzymes acting on damaged DNA. Nature. 1976 Jan 1;259(5538):64–66. doi: 10.1038/259064a0. [DOI] [PubMed] [Google Scholar]
  7. Lindahl T., Nyberg B. Heat-induced deamination of cytosine residues in deoxyribonucleic acid. Biochemistry. 1974 Jul 30;13(16):3405–3410. doi: 10.1021/bi00713a035. [DOI] [PubMed] [Google Scholar]
  8. Tomita F. Changes in DNase activities in Bacillus subtilis infected with bacteriophage PBS 1. J Virol. 1975 May;15(5):1073–1080. doi: 10.1128/jvi.15.5.1073-1080.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Tomita F., Takahashi I. DNase Specific for Uracil-Containing Bacteriophage DNA. J Virol. 1975 May;15(5):1081–1087. doi: 10.1128/jvi.15.5.1081-1087.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Verly W. G., Paquette Y. An endonuclease for depurinated DNA in Escherichia coli B. Can J Biochem. 1972 Feb;50(2):217–224. doi: 10.1139/o72-029. [DOI] [PubMed] [Google Scholar]
  11. Verly W. G., Rassart E. Purification of Escherichia coli endonuclease specific for apurinic sites in DNA. J Biol Chem. 1975 Oct 25;250(20):8214–8219. [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES