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. 1973 Jan;11(1):9–16. doi: 10.1128/jvi.11.1.9-16.1973

Transcription During the Development of Bacteriophage φ29: Definition of “Early” and “Late” φ29 Ribonucleic Acid

Charles F Schachtele 1, Carol V De Sain 1, Dwight L Anderson 1
PMCID: PMC355054  PMID: 4630804

Abstract

Bacteriophage φ29 messenger ribonucleic acid (mRNA) production following infection of Bacillus subtilis has been analyzed. Early (e) φ29 RNA, made prior to the onset of phage deoxyribonucleic acid (DNA) replication and exclusively from the light (L) φ29 DNA strand, has been shown by RNA-DNA hybridization-competition experiments to be present throughout the phage latent period. No repression of e RNA production during φ29 development could be demonstrated. Unmodified host RNA polymerase molecules appear to be sufficient for the synthesis of e RNA since phage-specific RNA made in the presence of chloramphenicol hybridizes only to the L strand of φ29 DNA, and this RNA can be effectively competed during hybridization by e RNA. The appearance of late (l) φ29 RNA is coincident with the onset of viral DNA replication. This RNA consists of L DNA strand transcripts which are identical to e RNA and a new class of mRNAs made exclusively from the “heavy” (H) φ29 DNA strand (lH). Protein synthesis in infected cells is required for lH RNA production. Studies with the antibiotic rifamycin demonstrated that synthesis of the major φ29 structural proteins is dependent on production of lH RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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