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. 1973 Feb;11(2):243–249. doi: 10.1128/jvi.11.2.243-249.1973

Stimulation of Sendai Virion Transcriptase by Polyanions

Henry O Stone 1, D W Kingsbury 1
PMCID: PMC355089  PMID: 4347427

Abstract

Exogenous polyribonucleotides stimulated the ribonucleic acid (RNA) transcriptase in Sendai virions. Added yeast RNA, polyadenylic acid, or polycytidylic acid increased incorporation of 3H-guanosine monophosphate as much as fivefold. The products of stimulated reactions were virus-specific as determined by hybridization with Sendai virion RNA, but they sedimented more slowly (13s) than the product of an unstimulated reaction (16s). The stimulating activity was nondialyzable and heat stable, but was abolished by alkaline hydrolysis. Nucleoside monophosphates, individually or in combination, were ineffective, confirming the requirement for a polymer. Among other substances tested for effects on Sendai virion transcriptase, polyaspartic acid and polyglutamic acid stimulated the enzyme; polyinosinic acid, polyuridylic acid, and polyamines had no effect; and dextran sulfate and polyvinyl sulfate were inhibitory.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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