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. 1973 May;11(5):655–664. doi: 10.1128/jvi.11.5.655-664.1973

Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses V. Isolation of Additional Hybrids Which Differ in Their Simian Virus 40-Specific Biological Properties

Andrew M Lewis Jr 1,2,3,4, Arthur S Levine 1,2,3,4, Clyde S Crumpacker 1,2,3,4,1, Myron J Levin 1,2,3,4, Richard J Samaha 1,2,3,4, Patrick H Henry 1,2,3,4
PMCID: PMC355161  PMID: 4350710

Abstract

Four new nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses have been isolated. Although these viruses (designated Ad2+ND2, Ad2+ND3, Ad2+ND4, and Ad2+ND5) were clonal derivatives of the same Ad2-SV40 hybrid population, they differ significantly from each other and from the previously isolated nondefective hybrid, Ad2+ND1, in their biological properties or in the amount of SV40-specific RNA induced during lytic infection.

Like Ad2+ND1, Ad2+ND2, and Ad2+ND4 pass serially in both human embryonic kidney (HEK) and primary African green monkey kidney cells. In contrast, Ad2+ND3 and Ad2+ND5 pass serially only in HEK cells. Ad2+ND2 is like Ad2+ND1 in that it induces the SV40 U antigen, but not SV40 T antigen; however, in contrast to the perinuclear SV40 antigen induced by Ad2+ND1, the SV40 antigen induced by Ad2+ND2 is located peripherally in the cytoplasm as well as in the perinuclear region of infected cells. Ad2+ND4 induces both the SV40 T and U antigens. Ad2+ND3 and Ad2+ND5 do not induce serologically detectable SV40 antigens and are distinguished from each other on the basis of the relative quantities of SV40-specific RNA which they induce. The induction of different SV40-specific functions suggests the incorporation of different segments of SV40 DNA within the genomes of the respective hybrid viruses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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