Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1973 May;11(5):694–701. doi: 10.1128/jvi.11.5.694-701.1973

Preparation and Properties of an Inhibitory Extract from Frog Virus 3 Particles

A-M Aubertin 1, C Hirth 1, C Travo 1, H Nonnenmacher 1, A Kirn 1
PMCID: PMC355165  PMID: 4736109

Abstract

The structural constituents of the frog virus 3 particle were solubilized by treatment with a nonionic detergent followed by the addition of a high salt concentration. This soluble viral extract (SVE) inhibits host nucleic acid synthesis. Its activity on RNA synthesis was studied in KB cells and found to be dependent on the presence of DEAE dextran. Inactivation of the inhibitory properties of SVE were obtained by trypsin digestion, treatment with urea, or heat denaturation. Neutralization of the activity of SVE was obtained by anti-frog virus 3 serum but not by anti-BHK serum. In vitro a complex may be formed between polynucleotides and the inhibitor indicating a possible mechanism for vivo inhibition.

Full text

PDF
695

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aubertin A. M., Decker C., Kirn A. Effect of gamma-rays on infectivity and capacity for nuclear-associated and cytoplasmic DNA replication of FV 3 in chick embryo fibroblasts. Radiat Res. 1970 Oct;44(1):178–186. [PubMed] [Google Scholar]
  2. Bingen-Brendel A., Batzenschlager A., Gut J. P., Hirth C., Vetter J. M., Kirn A. Etude histologique et virologique de l'hépatite dégénérative aigue provoquée par le FV3 (Frog Virus 3) chez la souris. Ann Inst Pasteur (Paris) 1972 Jan;122(1):125–142. [PubMed] [Google Scholar]
  3. Costanzo F., La Placa M., Palenzoma A. M., Novello F., Stirpe F. Selective inhibition of a nuclear RNA polymerase in cells infected with frog virus. Nature. 1970 Jul 18;227(5255):294–295. doi: 10.1038/227294a0. [DOI] [PubMed] [Google Scholar]
  4. Ensminger W. D., Tamm I. The step in cellular DNA synthesis blocked by Newcastle disease or mengovirus infection. Virology. 1970 Jan;40(1):152–165. doi: 10.1016/0042-6822(70)90387-9. [DOI] [PubMed] [Google Scholar]
  5. Ensminger W. D., Tamm I. The step in cellular DNA synthesis blocked by reovirus infection. Virology. 1969 Dec;39(4):935–938. doi: 10.1016/0042-6822(69)90032-4. [DOI] [PubMed] [Google Scholar]
  6. Gravell M., Cromeans T. L. Viron-associated protein kinase and its involvement in nongenetic reactivation of frog polyhedral cytoplasmic deoxyribovirus. Virology. 1972 Jun;48(3):847–851. doi: 10.1016/0042-6822(72)90167-5. [DOI] [PubMed] [Google Scholar]
  7. Guir J., Braunwald J., Kirn A. Inhibition of host-specific DNA and RNA synthesis in KB cells following infection with frog virus 3. J Gen Virol. 1971 Sep;12(3):293–301. doi: 10.1099/0022-1317-12-3-293. [DOI] [PubMed] [Google Scholar]
  8. Hodge L. D., Scharff M. D. Effect of adenovirus on host cell DNA synthesis in synchronized cells. Virology. 1969 Apr;37(4):554–564. doi: 10.1016/0042-6822(69)90273-6. [DOI] [PubMed] [Google Scholar]
  9. Jungwirth C., Launer J. Effect of poxvirus infection on host cell deoxyribonucleic acid synthesis. J Virol. 1968 May;2(5):401–408. doi: 10.1128/jvi.2.5.401-408.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kirn A., Gut J. P., Bingen A., Hirth C. Acute hepatitis produced by frog virus 3 in mice. Brief report. Arch Gesamte Virusforsch. 1972;36(3):394–397. doi: 10.1007/BF01249871. [DOI] [PubMed] [Google Scholar]
  11. Kirn A., Steffan A. M., Gut J. P. Stimulation du pouvoir neutralisant des immunsérums FV3 par le sérum frais de cobaye. Ann Inst Pasteur (Paris) 1972 Aug;123(2):265–273. [PubMed] [Google Scholar]
  12. Levine A. J., Ginsberg H. S. Mechanism by which fiber antigen inhibits multiplication of type 5 adenovirus. J Virol. 1967 Aug;1(4):747–757. doi: 10.1128/jvi.1.4.747-757.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Levine A. J., Ginsberg H. S. Role of adenovirus structural proteins in the cessation of host-cell biosynthetic functions. J Virol. 1968 May;2(5):430–439. doi: 10.1128/jvi.2.5.430-439.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Maes R., Granoff A. Viruses and renal carcinoma of Rana pipiens. IV. Nucleic acid synthesis in frog virus 3-infected BHK 21/13 cells. Virology. 1967 Nov;33(3):491–502. doi: 10.1016/0042-6822(67)90125-0. [DOI] [PubMed] [Google Scholar]
  15. McAuslan B. R., Smith W. R. Deoxyribonucleic acid synthesis in FV-3-infected mammalian cells. J Virol. 1968 Oct;2(10):1006–1015. doi: 10.1128/jvi.2.10.1006-1015.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Morrison J. F. Chromatographic separation of nucleoside phosphates on diethylaminoethylcellulose paper. Anal Biochem. 1968 Jul;24(1):106–111. doi: 10.1016/0003-2697(68)90064-x. [DOI] [PubMed] [Google Scholar]
  17. Palese P., Koch G. Degradation of single- and double-stranded RNA by frog virus 3. Proc Natl Acad Sci U S A. 1972 Mar;69(3):698–701. doi: 10.1073/pnas.69.3.698. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Palese P., McAuslan B. R. Virus-associated DNase: endonuclease in a polyhedral cytoplasmic deoxyvirus. Virology. 1972 Jul;49(1):319–321. doi: 10.1016/s0042-6822(72)80036-9. [DOI] [PubMed] [Google Scholar]
  19. Suarez H. G., Sonenshein G. E., Estrade S., Bourali M. F., Cassingena R., Tournier P. Activation of the viral genome in Simian Virus 40-transformed nonpermissive cells by permissive cell extracts. Proc Natl Acad Sci U S A. 1972 May;69(5):1290–1293. doi: 10.1073/pnas.69.5.1290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vilagines R., McAuslan B. R. Proteins of polyhedal cytoplasmic deoxyvirus. II. Nucleotide phosphohydrolase activity associated with frog virus 3. J Virol. 1971 May;7(5):619–624. doi: 10.1128/jvi.7.5.619-624.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yaoi Y., Amano M. Inhibitory effect of ultraviolet-inactivated vesicular stomatitis virus on initiation o DNA synthesis in cultured chick embryo cells. J Gen Virol. 1970 Oct;9(1):69–75. doi: 10.1099/0022-1317-9-1-69. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES