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. 1973 May;11(5):741–747. doi: 10.1128/jvi.11.5.741-747.1973

Relationship of Reticuloendotheliosis Virus to the Avian Tumor Viruses: Nucleic Acid and Polypeptide Composition

Reynaldo L Maldonado 1, Henry R Bose 1
PMCID: PMC355171  PMID: 4350718

Abstract

The RNA content and polypeptide composition of reticuloendotheliosis virus (REV) was compared to that of C-type RNA tumor viruses. Two RNA species with approximate sedimentation values of 64S and 4S were observed after sucrose gradient centrifugation of RNA extracted from purified REV. The high-molecular-weight RNA species of REV sedimented slightly faster than that of the Bryan strain of Rous sarcoma virus (RSV). Although these characteristics were consistent with those of other C-type RNA tumor viruses, significant differences were observed when the polypeptide composition of REV was compared with that of RSV possessing envelope determinants of Rous-associated virus RAV-2 and RAV-3. Five polypeptides of which two were glycosylated were resolved by polyacrylamide gel electrophoresis. The major nonglycosylated polypeptide of REV did not comigrate with that of RSV (RAV-2)-RSV(RAV-3). The majority of the group-specific antigen reactivity resides in this major nonglycosylated polypeptide of avian tumor viruses and comigrates when proteins of several avian tumor viruses are subjected to coelectrophoresis. This difference in the migration of the major polypeptide of REV and RSV(RAV-2)-RSV(RAV-3) may explain the absence of avian tumor virus group-specific antigen in REV.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen D. W., Sarma P. S., Niall H. D., Sauer R. Isolation of a second avian leukosis group-specific antigen (gs-b) from avian myeloblastosis virus. Proc Natl Acad Sci U S A. 1970 Oct;67(2):837–842. doi: 10.1073/pnas.67.2.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baxter-Gabbard K. L., Campbell W. F., Padgett F., Raitano-Fenton A., Levin A. S. Avian reticuloendotheliosis virus (strain T). II. Biochemical and biophysical properties. Avian Dis. 1971 Oct-Dec;15(4):850–862. [PubMed] [Google Scholar]
  3. Bishop J. M., Levinson W. E., Quintrell N., Sullivan D., Fanshier L., Jackson J. The low molecular weight RNAs of Rous sarcoma virus. I. The 4 S RNA. Virology. 1970 Sep;42(1):182–195. doi: 10.1016/0042-6822(70)90251-5. [DOI] [PubMed] [Google Scholar]
  4. Bolognesi D. P., Graf T. Size differences among the high molecular weight RNA's of avian tumor viruses. Virology. 1971 Jan;43(1):214–222. doi: 10.1016/0042-6822(71)90239-x. [DOI] [PubMed] [Google Scholar]
  5. Bonar R. A., Sverak L., Bolognesi D. P., Langlois A. J., Beard D., Beard J. W. Ribonucleic acid components of BAI strain A (myeloblastosis) avian tumor virus. Cancer Res. 1967 Jun;27(6):1138–1157. [PubMed] [Google Scholar]
  6. Bose H. R., Jr, Levine A. S. Replication of the reticuloendotheliosis virus (strain T) in chicken embryo cell culture. J Virol. 1967 Dec;1(6):1117–1121. doi: 10.1128/jvi.1.6.1117-1121.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Campbell W. F., Baxter-Gabbard K. L., Levine A. S. Avian reticuloendotheliosis virus (strain T). I. Virological characterization. Avian Dis. 1971 Oct-Dec;15(4):837–849. [PubMed] [Google Scholar]
  8. Duesberg P. H., Martin G. S., Vogt P. K. Glycoprotein components of avian and murine RNA tumor viruses. Virology. 1970 Aug;41(4):631–646. doi: 10.1016/0042-6822(70)90428-9. [DOI] [PubMed] [Google Scholar]
  9. Geering G., Aoki T., Old L. J. Shared viral antigen of mammalian leukaemia viruses. Nature. 1970 Apr 18;226(5242):265–266. doi: 10.1038/226265a0. [DOI] [PubMed] [Google Scholar]
  10. Geering G., Hardy W. D., Jr, Old L. J., de Harven E., Brodey R. S. Shared group-specific antigen of murine and feline leukemia viruses. Virology. 1968 Dec;36(4):678–680. doi: 10.1016/0042-6822(68)90199-2. [DOI] [PubMed] [Google Scholar]
  11. Hay A. J., Skehel J. J., Burke D. C. Proteins synthesized in chick cells following infection with Semliki Forest virus. J Gen Virol. 1968 Sep;3(2):175–184. doi: 10.1099/0022-1317-3-2-175. [DOI] [PubMed] [Google Scholar]
  12. Hung P. P., Robinson H. L., Robinson W. S. Isolation and characterization of proteins from Rous sarcoma virus. Virology. 1971 Jan;43(1):251–266. doi: 10.1016/0042-6822(71)90243-1. [DOI] [PubMed] [Google Scholar]
  13. Johnston R. E., Bose H. R. An adenylate-rich segment in the virion RNA of Sindbis virus. Biochem Biophys Res Commun. 1972 Jan 31;46(2):712–718. doi: 10.1016/s0006-291x(72)80198-0. [DOI] [PubMed] [Google Scholar]
  14. Kang C. Y., Temin H. M. Endogenous RNA-directed DNA polymerase activity in uninfected chicken embryos. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1550–1554. doi: 10.1073/pnas.69.6.1550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Maldonado R. L., Bose H. R. Separation of reticuloendotheliosis virus from avian tumor viruses. J Virol. 1971 Nov;8(5):813–815. doi: 10.1128/jvi.8.5.813-815.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Old L. J., Boyse E. A., Geering G., Oettgen H. F. Serologic approaches to the study of cancer in animals and in man. Cancer Res. 1968 Jul;28(7):1288–1299. [PubMed] [Google Scholar]
  17. Oroszlan S., Foreman C., Kelloff G., Gilden R. V. The group-specific antigen and other structural proteins of hamster and mouse C-type viruses. Virology. 1971 Mar;43(3):665–674. doi: 10.1016/0042-6822(71)90290-x. [DOI] [PubMed] [Google Scholar]
  18. Oroszlan S., Huebner R. J., Gilden R. V. Species-specific and interspecific antigenic determinants associated with the structural protein of feline C-type virus. Proc Natl Acad Sci U S A. 1971 May;68(5):901–904. doi: 10.1073/pnas.68.5.901. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Padgett F., Baxter-Gabbard K. L., Raitano-Fenton A., Levine A. S. Avian reticuloendotheliosis virus (strain T). 3. Ultrastructure studies. Avian Dis. 1971 Oct-Dec;15(4):863–873. [PubMed] [Google Scholar]
  20. Peterson D. A., Baxter-Gabbard K. L., Levine A. S. Avian reticuloendotheliosis virus (strain T): V. DNA polymerase. Virology. 1972 Jan;47(1):251–254. doi: 10.1016/0042-6822(72)90259-0. [DOI] [PubMed] [Google Scholar]
  21. Robinson W. S., Hung P., Robinson H. L., Ralph D. D. Proteins of avian tumor viruses with different coat antigens. J Virol. 1970 Nov;6(5):695–698. doi: 10.1128/jvi.6.5.695-698.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Roth F. K., Dougherty R. M. Multiple antigenic components of the group-specific antigen of the avian leukosis-sarcoma viruses. Virology. 1969 Jun;38(2):278–284. doi: 10.1016/0042-6822(69)90369-9. [DOI] [PubMed] [Google Scholar]
  23. Scheele C. M., Hanafusa H. Proteins of helper-dependent RSV. Virology. 1971 Aug;45(2):401–410. doi: 10.1016/0042-6822(71)90341-2. [DOI] [PubMed] [Google Scholar]
  24. Theilen G. H., Zeigel R. F., Twiehaus M. J. Biological studies with RE virus (strain T) that induces reticuloendotheliosis in turkeys, chickens, and Japanese quail. J Natl Cancer Inst. 1966 Dec;37(6):731–743. [PubMed] [Google Scholar]
  25. Vogt P. K., Ishizaki R. Patterns of viral interference in the avian leukosis and sarcoma complex. Virology. 1966 Nov;30(3):368–374. doi: 10.1016/0042-6822(66)90115-2. [DOI] [PubMed] [Google Scholar]
  26. Zeigel R. F., Theilen G. H., Twiehaus M. J. Electron microscopic observations on RE virus (strain T) that induces reticuloendotheliosis in turkeys, chickens, and Japanese quail. J Natl Cancer Inst. 1966 Dec;37(6):709–729. [PubMed] [Google Scholar]

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