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. 1974 Jan;13(1):73–80. doi: 10.1128/jvi.13.1.73-80.1974

Localization of Membrane Protein Synthesized After Infection with Bacteriophage T4

Gail Fletcher 1, Judith L Wulff 1, C F Earhart 1
PMCID: PMC355260  PMID: 4590020

Abstract

The synthesis of membrane protein after infection with bacteriophage T4 was examined. Protein constituents of both the cytoplasmic and outer membrane are made during the infective cycle. In addition, newly synthesized membrane protein is found in material which has a buoyant density greater than that of either of the two host membrane fractions. Polyacrylamide gel analyses and solubilization studies using the detergent Sarkosyl indicate that synthesis of most of the membrane proteins made during the first 5 min of infection is directed by bacterial genes. New membrane proteins synthesized at times greater than 6 min after infection appear to be distinct from those of the host, and new proteins of the outer membrane are different from those of the inner. Proteins in the new dense membrane fraction are similar to those of the outer membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DULBECCO R. Mutual exclusion between related phages. J Bacteriol. 1952 Feb;63(2):209–217. doi: 10.1128/jb.63.2.209-217.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DePamphilis M. L., Adler J. Attachment of flagellar basal bodies to the cell envelope: specific attachment to the outer, lipopolysaccharide membrane and the cyoplasmic membrane. J Bacteriol. 1971 Jan;105(1):396–407. doi: 10.1128/jb.105.1.396-407.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Duckworth D. H. Inhibition of T4 bacteriophage multiplication by superinfecting ghosts and the development of tolerance after bacteriophage infection. J Virol. 1971 Jan;7(1):8–14. doi: 10.1128/jvi.7.1.8-14.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Earhart C. F. The association of host and phage DNA with the membrane of Escherichia coli. Virology. 1970 Oct;42(2):420–436. [PubMed] [Google Scholar]
  5. Emrich J. Lysis of T4-infected bacteria in the absence of lysozyme. Virology. 1968 May;35(1):158–165. doi: 10.1016/0042-6822(68)90315-2. [DOI] [PubMed] [Google Scholar]
  6. Ennis H. L., Kievitt K. D. Association of the rIIA protein with the bacterial membrane. Proc Natl Acad Sci U S A. 1973 May;70(5):1468–1472. doi: 10.1073/pnas.70.5.1468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Filip C., Fletcher G., Wulff J. L., Earhart C. F. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. J Bacteriol. 1973 Sep;115(3):717–722. doi: 10.1128/jb.115.3.717-722.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Furrow M. H., Pizer L. I. Phospholipid synthesis in Escherichia coli infected with T4 bacteriophages. J Virol. 1968 Jun;2(6):594–605. doi: 10.1128/jvi.2.6.594-605.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Glauert A. M., Thornley M. J. The topography of the bacterial cell wall. Annu Rev Microbiol. 1969;23:159–198. doi: 10.1146/annurev.mi.23.100169.001111. [DOI] [PubMed] [Google Scholar]
  10. Inouye M., Guthrie J. P. A mutation which changes a membrane protein of E. coli. Proc Natl Acad Sci U S A. 1969 Nov;64(3):957–961. doi: 10.1073/pnas.64.3.957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Inouye M., Yee M. L. Homogeneity of envelope proteins of Escherichia coli separated by gel electrophoresis in sodium dodecyl sulfate. J Bacteriol. 1973 Jan;113(1):304–312. doi: 10.1128/jb.113.1.304-312.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Munch-Petersen A., Schwartz M. Inhibition of the catabolism of deoxyribonucleosides in Escherichia coli after infection by T 4 phage. Eur J Biochem. 1972 Jun 9;27(3):443–447. doi: 10.1111/j.1432-1033.1972.tb01858.x. [DOI] [PubMed] [Google Scholar]
  14. Osborn M. J., Gander J. E., Parisi E., Carson J. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. J Biol Chem. 1972 Jun 25;247(12):3962–3972. [PubMed] [Google Scholar]
  15. Peterson R. F., Kievitt K. D., Ennis H. L. Membrane protein synthesis after infection of Escherichia coli B with phage T4: the rIIB protein. Virology. 1972 Nov;50(2):520–527. doi: 10.1016/0042-6822(72)90403-5. [DOI] [PubMed] [Google Scholar]
  16. Peterson R. H., Buller C. S. Phospholipid metabolism in T4 bacteriophage infected Escherichia coli K-12 (lambda). J Virol. 1969 May;3(5):463–468. doi: 10.1128/jvi.3.5.463-468.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. ROTHFIELD L., OSBORN M. J., HORECKER B. L. BIOSYNTHESIS OF BACTERIAL LIPOPOLYSACCHARIDE. II. INCORPORATION OF GLUCOSE AND GALACTOSE CATALYZED BY PARTICULATE AND SOLUBLE ENZYMES IN SALMONELLA. J Biol Chem. 1964 Sep;239:2788–2795. [PubMed] [Google Scholar]
  18. SISTROM W. R. On the physical state of the intracellularly accumulates substrates of beta-galactoside-permease in Escherichia coli. Biochim Biophys Acta. 1958 Sep;29(3):579–587. doi: 10.1016/0006-3002(58)90015-5. [DOI] [PubMed] [Google Scholar]
  19. Schnaitman C. A. Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli. J Bacteriol. 1970 Nov;104(2):890–901. doi: 10.1128/jb.104.2.890-901.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schnaitman C. A. Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100. J Bacteriol. 1971 Oct;108(1):545–552. doi: 10.1128/jb.108.1.545-552.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Silver S. Acridine sensitivity of bacteriophage T2: a virus gene affecting cell permeability. J Mol Biol. 1967 Oct 14;29(1):191–202. doi: 10.1016/0022-2836(67)90190-8. [DOI] [PubMed] [Google Scholar]
  22. Silver S., Levine E., Spielman P. M. Cation fluxes and permeability changes accompanying bacteriophage infection of Escherichia coli. J Virol. 1968 Aug;2(8):763–771. doi: 10.1128/jvi.2.8.763-771.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Simon L. D. Infection of Escherichia coli by T2 and T4 bacteriophages as seen in the electron microscope: T4 head morphogenesis. Proc Natl Acad Sci U S A. 1972 Apr;69(4):907–911. doi: 10.1073/pnas.69.4.907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Takano T., Kakefuda T. Involvement of a bacterial factor in morphogenesis of bacteriophage capsid. Nat New Biol. 1972 Sep 13;239(89):34–37. doi: 10.1038/newbio239034a0. [DOI] [PubMed] [Google Scholar]
  25. VISCONTI N. Resistance to lysis from without in bacteria infected with T2 bacteriophage. J Bacteriol. 1953 Sep;66(3):247–253. doi: 10.1128/jb.66.3.247-253.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Vallée M., Cornett J. B. A new gene of bacteriophage T4 determining immunity against superinfecting ghosts and phage in T4-infected Escherichia coli. Virology. 1972 Jun;48(3):777–784. doi: 10.1016/0042-6822(72)90161-4. [DOI] [PubMed] [Google Scholar]
  27. Weintraub S. B., Frankel F. R. Identification of the T4rIIB gene product as a membrane protein. J Mol Biol. 1972 Oct 14;70(3):589–615. doi: 10.1016/0022-2836(72)90561-x. [DOI] [PubMed] [Google Scholar]
  28. Wu H. C. Isolation and characterization of an Escherichia coli mutant with alteration in the outer membrane porteins of the cell envelope. Biochim Biophys Acta. 1972 Dec 1;290(1):274–289. doi: 10.1016/0005-2736(72)90070-3. [DOI] [PubMed] [Google Scholar]

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