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. 1974 Feb;13(2):400–410. doi: 10.1128/jvi.13.2.400-410.1974

Replication Process of the Parvovirus H-1 II. Isolation and Characterization of H-1 Replicative Form DNA

Solon L Rhode III 1
PMCID: PMC355310  PMID: 4855740

Abstract

The Parvovirus H-1 replicates autonomously in hamster embryo cells. A DNA synthetic event, called HA-DNA synthesis, upon which subsequent viral RNA and viral hemagglutinin synthesis is dependent, is initiated in late S phase of the infected cell (18). It was postulated that HA-DNA represents parental viral replicative form DNA (RF DNA). This study describes the isolation and characterization of H-1 RF DNA as part of the continuing study of the mechanisms and control of DNA replication in the eukaryotic cell. The H-1 RF DNA is a linear duplex molecule containing the viral strand and its complement. The complementary strands of the RF DNA have been separated by equilibrium density gradient centrifugation. The RF DNA has a buoyant density of 1.705 in neutral CsCl and an estimated guanine plus cytosine (GC) content of 45.9%. It has a sedimentation coefficient of 17S. The calculated molecular weight of 3.7 × 106 is twice that of the single-stranded virion DNA. H-1 virions contain DNA that is homogeneous and free of complementary strands.

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Selected References

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