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. 1974 Apr;13(4):881–887. doi: 10.1128/jvi.13.4.881-887.1974

Effect of d-Penicillamine on Poliovirus Replication In HeLa Cells

Parvin Merryman 1, Israeli A Jaffe 1, Ellie Ehrenfeld 1
PMCID: PMC355386  PMID: 4362514

Abstract

A series of mercaptan compounds were studied with respect to their ability to inhibit the growth of poliovirus in cultured cells. Of the compounds tested only d-penicillamine possessed antiviral activity. There was no direct effect on the virus itself, nor were the processes of adsorption, penetration and uncoating, or virus-induced “shut-off” of host cell protein synthesis inhibited. At concentrations where there was no effect on host cell RNA or protein synthesis, d-penicillamine caused a marked inhibition of virus-specific RNA and protein synthesis. Although much reduced, the relative concentrations of single-stranded and double-stranded viral RNA synthesized in the presence of d-penicillamine was unchanged. Similarly, all apparent precursor and cleavage product proteins could be synthesized in the presence of the drug. The inhibitory effect was reversible, after a lag of 1.5 to 2 h after removal of the drug, and normal yields of virus could be obtained. The structural and functional properties of d-penicillamine are discussed in relation to requirements for anti-polioviral activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D., Girard M., Darnell J. E. Aspects of the synthesis of poliovirus RNA and the formation of virus particles. Virology. 1966 Jun;29(2):179–189. doi: 10.1016/0042-6822(66)90024-9. [DOI] [PubMed] [Google Scholar]
  2. Baum S. G., Horwitz M. S., Maizel J. V., Jr Studies of the mechanism of enhancement of human adenovirus infection in monkey cells by simian virus 40. J Virol. 1972 Aug;10(2):211–219. doi: 10.1128/jvi.10.2.211-219.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CHRISTIAN C. L. RHEUMATOID ARTHRITIS--ETIOLOGIC CONSIDERATIONS. Arthritis Rheum. 1964 Oct;7:455–466. doi: 10.1002/art.1780070502. [DOI] [PubMed] [Google Scholar]
  4. CRAWHALL J. C., SCOWEN E. F., WATTS R. W. Effect of penicillamine on cystinuria. Br Med J. 1963 Mar 2;1(5330):588–590. doi: 10.1136/bmj.1.5330.588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CROWTHER D., MELNICK J. L. Studies of the inhibitory action of guanidine on poliovirus multiplication in cell cultures. Virology. 1961 Sep;15:65–74. doi: 10.1016/0042-6822(61)90078-2. [DOI] [PubMed] [Google Scholar]
  6. Gessa G. L., Loddo B., Brotzu G., Schivo M. L., Tagliamonte A., Spanedda A., Bo G., Ferrari W. Selective inhibition of poliovirus growth by d-Penicillamine in vitro. Virology. 1966 Dec;30(4):618–622. doi: 10.1016/0042-6822(66)90166-8. [DOI] [PubMed] [Google Scholar]
  7. JAFFE I. A., ALTMAN K., MERRYMAN P. THE ANTIPYRIDOXINE EFFECT OF PENICILLAMINE IN MAN. J Clin Invest. 1964 Oct;43:1869–1873. doi: 10.1172/JCI105060. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jacobson M. F., Baltimore D. Polypeptide cleavages in the formation of poliovirus proteins. Proc Natl Acad Sci U S A. 1968 Sep;61(1):77–84. doi: 10.1073/pnas.61.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jaffe I. A. The treatment of rheumatoid arthritis and necrotizing vasculitis with penicillamine. Arthritis Rheum. 1970 Jul-Aug;13(4):436–443. doi: 10.1002/art.1780130412. [DOI] [PubMed] [Google Scholar]
  10. KUCHINSKAS E. J., HORVATH A., DU VIGNEAUD V. An anti-vitamin B6 action of L-penicillamine. Arch Biochem Biophys. 1957 May;68(1):69–75. doi: 10.1016/0003-9861(57)90327-2. [DOI] [PubMed] [Google Scholar]
  11. La Colla P., Marcialis M. A., Mereu G. P., Loddo B. Specific inhibition of poliovirus induced blockade of cell protein synthesis by a thiopyrimidine derivative. J Gen Virol. 1972 Oct;17(1):13–18. doi: 10.1099/0022-1317-17-1-13. [DOI] [PubMed] [Google Scholar]
  12. Nimni M. E., Bavetta L. A. Collagen defect induced by penicillamine. Science. 1965 Nov 12;150(3698):905–907. doi: 10.1126/science.150.3698.905. [DOI] [PubMed] [Google Scholar]
  13. Penman S., Summers D. Effects on host cell metabolism following synchronous infection with poliovirus. Virology. 1965 Dec;27(4):614–620. doi: 10.1016/0042-6822(65)90187-x. [DOI] [PubMed] [Google Scholar]
  14. Studier F. W. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. doi: 10.1016/0022-2836(73)90003-x. [DOI] [PubMed] [Google Scholar]
  15. Summers D. F., Maizel J. V., Jr, Darnell J. E., Jr Evidence for virus-specific noncapsid proteins in poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1965 Aug;54(2):505–513. doi: 10.1073/pnas.54.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. WALSHE J. M. Disturbances of aminoacid metabolism following liver injury; a study by means of paper chromatography. Q J Med. 1953 Oct;22(88):483–505. [PubMed] [Google Scholar]

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