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. 1974 May;13(5):1093–1100. doi: 10.1128/jvi.13.5.1093-1100.1974

Evidence for Heterogeneity in Populations of T5 Bacteriophage

B Labedan 1, J Legault-Demare 1
PMCID: PMC355419  PMID: 4596294

Abstract

Each T5 stock contains a population of particular phages that, just after adsorption onto the host bacteria. release their entire chromosome outside the bacterial membrane in a place where it is sensitive to bacterial enzymes. This release takes place before the sensitization step to deprivation of calcium and before the transfer of the first-step DNA fragment. Secondarily, this released DNA is degraded by bacterial enzymes, mainly by the endonuclease I; the products of degradation are spontaneously released in the surrounding medium. Thus, in each T5 phage stock it seems that there is a minor population that is deficient for the mechanism of controlled DNA injection into the bacteria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson C. W., Eigner J. Breakdown and exclusion of superinfecting T-even bacteriophage in Escherichia coli. J Virol. 1971 Dec;8(6):869–886. doi: 10.1128/jvi.8.6.869-886.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bayer M. E. Adsorption of bacteriophages to adhesions between wall and membrane of Escherichia coli. J Virol. 1968 Apr;2(4):346–356. doi: 10.1128/jvi.2.4.346-356.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Crothers D. M., Zimm B. H. Viscosity and sedimentation of the DNA from bacteriophages T2 and T7 and the relation to molecular weight. J Mol Biol. 1965 Jul;12(3):525–536. doi: 10.1016/s0022-2836(65)80310-2. [DOI] [PubMed] [Google Scholar]
  4. Dürwald H., Hoffmann-Berling H. Endonuclease-I-deficient and ribonuclease I-deficient Escherichia coli mutants. J Mol Biol. 1968 Jul 14;34(2):331–346. doi: 10.1016/0022-2836(68)90257-x. [DOI] [PubMed] [Google Scholar]
  5. Fielding P. E., Lunt M. R. The relation between breakdown of superinfecting virus deoxyribonucleic acid and temporal exclusion induced by T4 and T5 bacteriophages. J Gen Virol. 1970 Mar;6(3):333–342. doi: 10.1099/0022-1317-6-3-333. [DOI] [PubMed] [Google Scholar]
  6. HERSHEY A. D., CHASE M. Independent functions of viral protein and nucleic acid in growth of bacteriophage. J Gen Physiol. 1952 May;36(1):39–56. doi: 10.1085/jgp.36.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Heppel L. A. Selective release of enzymes from bacteria. Science. 1967 Jun 16;156(3781):1451–1455. doi: 10.1126/science.156.3781.1451. [DOI] [PubMed] [Google Scholar]
  8. Jacquemin-Sablon A., Richardson C. C. Analysis of the interruptions in bacteriophage T5 DNA. J Mol Biol. 1970 Feb 14;47(3):477–493. doi: 10.1016/0022-2836(70)90316-5. [DOI] [PubMed] [Google Scholar]
  9. LANNI Y. T. Infection by bacteriophage T5 and its intracellular growth; a study by complement fixation. J Bacteriol. 1954 Jun;67(6):640–650. doi: 10.1128/jb.67.6.640-650.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LANNI Y. T. Invasion by bacteriophage T5. I. Some basic kinetic features. Virology. 1960 Apr;10:501–513. doi: 10.1016/0042-6822(60)90132-x. [DOI] [PubMed] [Google Scholar]
  11. LANNI Y. T. Invasion by bacteriophage T5. II. Dissociation of calcium-independent and calcium-dependent processes. Virology. 1960 Apr;10:514–529. doi: 10.1016/0042-6822(60)90133-1. [DOI] [PubMed] [Google Scholar]
  12. LANNI Y. T. Invasion by bacteriophage T5. III. Stages revealed by changes in susceptibility of early complexes to abortive infection. Virology. 1961 Oct;15:127–135. doi: 10.1016/0042-6822(61)90229-x. [DOI] [PubMed] [Google Scholar]
  13. LEHMAN I. R., ROUSSOS G. G., PRATT E. A. The deoxyribonucleases of Escherichia coli. II. Purification and properties of a ribonucleic acid-inhibitable endonuclease. J Biol Chem. 1962 Mar;237:819–828. [PubMed] [Google Scholar]
  14. LURIA S. E., STEINER D. L. The role of calcium in the penetration of bacteriophage T5 into its host. J Bacteriol. 1954 Jun;67(6):635–639. doi: 10.1128/jb.67.6.635-639.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Labedan B., Crochet M., Legault-Demare J., Stevens B. J. Location of the first step transfer fragment and single-strand interruptions in T5stO bacteriophage DNA. J Mol Biol. 1973 Apr 5;75(2):213–234. doi: 10.1016/0022-2836(73)90017-x. [DOI] [PubMed] [Google Scholar]
  16. Labedan B., Legault-Demare J. Penetration into host cells of naked, partially injected (post-FST) DNA of bacteriophage T5. J Virol. 1973 Aug;12(2):226–229. doi: 10.1128/jvi.12.2.226-229.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lanni Y. T. DNA transfer from phage T5 to host cells: dependence on intercurrent protein synthesis. Proc Natl Acad Sci U S A. 1965 May;53(5):969–973. doi: 10.1073/pnas.53.5.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lanni Y. T. First-step-transfer deoxyribonucleic acid of bacteriophage T5. Bacteriol Rev. 1968 Sep;32(3):227–242. doi: 10.1128/br.32.3.227-242.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Leighton S. B., Rubenstein I. Calibration of molecular weight scales for DNA. J Mol Biol. 1969 Dec 14;46(2):313–328. doi: 10.1016/0022-2836(69)90424-0. [DOI] [PubMed] [Google Scholar]
  20. MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
  21. NEU H. C., HEPPEL L. A. THE RELEASE OF RIBONUCLEASE INTO THE MEDIUM WHEN ESCHERICHIA COLI CELLS ARE CONVERTED TO SPEROPLASTS. J Biol Chem. 1964 Nov;239:3893–3900. [PubMed] [Google Scholar]
  22. Nossal N. G., Heppel L. A. The release of enzymes by osmotic shock from Escherichia coli in exponential phase. J Biol Chem. 1966 Jul 10;241(13):3055–3062. [PubMed] [Google Scholar]
  23. WEIDEL W., KELLENBERGER E. The E. coli B-receptor for the phage T5. II. Electron microscopic studies. Biochim Biophys Acta. 1955 May;17(1):1–9. doi: 10.1016/0006-3002(55)90314-0. [DOI] [PubMed] [Google Scholar]
  24. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology. 1970 Mar;40(3):734–744. doi: 10.1016/0042-6822(70)90218-7. [DOI] [PubMed] [Google Scholar]

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