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. 1974 Jun;13(6):1338–1345. doi: 10.1128/jvi.13.6.1338-1345.1974

Poly(A) Polymerase Activity in Reovirus

C M Stoltzfus a,1, M Morgan a, A K Banerjee a, A J Shatkin a
PMCID: PMC355454  PMID: 4833612

Abstract

An enzymatic activity which synthesized oligo(A) in vitro was found in highly purified reovirus. The poly(A) polymerase activity was dependent on Mn2+ and utilized only ATP, whereas the virion-associated RNA polymerase required all four ribonucleoside triphosphates and Mg2+. Oligo(A) synthesis was demonstrated with complete virions and infectious subviral particles derived from virus by limited chymotrypsin digestion but not with cores, a product of extensive chymotrypsin digestion of virus. The enzymatic product and the oligo(A) from purified virions were isolated by binding to oligo(dT)-cellulose columns. Most of the in vitro product was similar in size and structure to the oligo(A) from purified virions by the criteria of gel electrophoresis, DEAE-cellulose chromatography, end-group analysis, and sensitivity to RNase. The evidence suggests that oligo(A) synthesis is mediated by the poly(A) polymerase during a late step in viral morphogenesis and may result from an alternative activity of the virion-associated transcriptase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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