Abstract
A DNA-containing bacteriophage, designated RS1, infecting Rhodopseudomonas spheroides 2.4.1, has been isolated from sewage. The buoyant density of RS1 in CsCl equilibrium centrifugation is 1.50 g/cm3, and the buoyant density of RS1 DNA is 1.706. The phage possesses a polyhedral head, approximately 65 nm in diameter, and a tail 60 nm long. When grown on aerobic cells, RS1 has a latent period of 120 min and an average burst size of 20. When grown on anaerobic cells, RS1 has a latent period of 150 min, and a burst size similar to that observed during aerobic infection. The adsorption rate constant of RS1 to aerobic cells is 1.2 × 10−9 ml/min, and 0.58 × 10−9 ml/min to anaerobic cells. Adsorption of RS1 to R. spheroides requires the presence of divalent cations.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
- CERIOTTI G. A microchemical determination of desoxyribonucleic acid. J Biol Chem. 1952 Sep;198(1):297–303. [PubMed] [Google Scholar]
- COHEN-BAZIRE G., SISTROM W. R., STANIER R. Y. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol. 1957 Feb;49(1):25–68. doi: 10.1002/jcp.1030490104. [DOI] [PubMed] [Google Scholar]
- Fraker P. J., Kaplan S. Isolation and fractionation of the photosynthetic membranous organelles from Rhodopseudomonas spheroides. J Bacteriol. 1971 Oct;108(1):465–473. doi: 10.1128/jb.108.1.465-473.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Freifelder D. Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M. J Mol Biol. 1970 Dec 28;54(3):567–577. doi: 10.1016/0022-2836(70)90127-0. [DOI] [PubMed] [Google Scholar]
- Freund-Mölbert E., Drews G., Bosecker K., Schubel B. Morphologie und Wirtskresi eines neu isolierten Rhodopseudomonas pallustris-Phagen. Arch Mikrobiol. 1968;64(1):1–8. [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. doi: 10.1016/s0022-2836(62)80066-7. [DOI] [PubMed] [Google Scholar]
- Rudner R., Shapiro H. S., Chargaff E. Studies on the nucleotide arrangement in deoxyribonucleic acids. X. Frequency and composition of pyrimidine isostichs in microbial deoxyribonucleic acids and in the DNA of E. coli phage T3. Biochim Biophys Acta. 1966 Oct 24;129(1):85–103. [PubMed] [Google Scholar]
- SISTROM W. R. A requirement for sodium in the growth of Rhodopseudomonas spheroides. J Gen Microbiol. 1960 Jun;22:778–785. doi: 10.1099/00221287-22-3-778. [DOI] [PubMed] [Google Scholar]
- SISTROM W. R. The kinetics of the synthesis of photopigments in Rhodopseudomonas spheroides. J Gen Microbiol. 1962 Sep;28:607–616. doi: 10.1099/00221287-28-4-607. [DOI] [PubMed] [Google Scholar]
- SUEOKA N., MARMUR J., DOTY P., 2nd Dependence of the density of deoxyribonucleic acids on guanine-cytosine content. Nature. 1959 May 23;183(4673):1429–1431. doi: 10.1038/1831429a0. [DOI] [PubMed] [Google Scholar]