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. 1974 Aug;14(2):375–383. doi: 10.1128/jvi.14.2.375-383.1974

In Vitro Synthesis of Adenovirus Core Proteins

Larry Eron 1, Heiner Wesphal 1, Robert Callahan 1
PMCID: PMC355523  PMID: 4211166

Abstract

mRNA extracted from polysomes of KB cells at late stages of productive infection with adenovirus type 2 was translated in a cell-free system derived from Krebs II ascites cells. Two of the polypeptides obtained in this reaction corresponded to the adenovirus core protein V and the precursor to core protein VII. The following criteria were used to establish identity between the in vitro products and the virion proteins: comigration during sodium dodecyl sulfatepolyacrylamide gel electrophoresis, cochromatography in sodium dodecyl sulfate-hydroxyapatite, specific immunoprecipitation of the precursor to core protein VII, and tryptic peptide analysis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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