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. 1974 Sep;14(3):509–516. doi: 10.1128/jvi.14.3.509-516.1974

Isolation and Characterization of Prototrophic Mutants of Escherichia coli Unable to Support the Intracellular Growth of T7

Michael Chamberlin 1
PMCID: PMC355544  PMID: 4604641

Abstract

Mutants of E. coli B/1 were isolated which grew normally but did not permit the intracellular growth of bacteriophage T7. Two classes of mutants were studied in detail (tsnB− and tsnC−). These strains adsorbed T7 normally and were killed by the infection. Synthesis of T7 RNA and of early and late classes of T7 proteins occurred normally after infection. In T7-infected tsnB− cells, T7 DNA synthesis stopped prematurely shortly after its onset, suggesting that the tsnB function affects a step in the late phase of T7 DNA replication. Mutants of T7 were isolated (T7β) which could grow on tsnB− cells. In T7-infected tsnC− cells, T7 DNA synthesis was completely blocked, suggesting that the tsnC function affects a step in an early phase of T7 DNA replication.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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