Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1974 Oct;14(4):733–739. doi: 10.1128/jvi.14.4.733-739.1974

Comparative Studies on Functions of Human Adenovirus Type 12 and Its Low Oncogenic Mutant Virions

Hisanori Ezoe 1, Stanley Mak 1
PMCID: PMC355576  PMID: 4607584

Abstract

Physical and biological properties of highly oncogenic human adenovirus type 12 were compared with a low oncogenic mutant (cyt mutant). Parental and cyt mutant virions had very similar density and DNA size. However, the parental strain virion preparations contained a much higher proportion of defective virions (capable of cell killing, but not able to induce T- or V-antigen) than cyt mutant stock. It was also found that cyt mutant had a reduced virus yield in several human cell lines compared with the parental strain.

Full text

PDF
736

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Butel J. S., Melnick J. L., Rapp F. Detection of biologically active adenovirions unable to plaque in human cells. J Bacteriol. 1966 Aug;92(2):433–438. doi: 10.1128/jb.92.2.433-438.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Defendi V., Jensen F. Oncogenicity by DNA tumor viruses: enhancement after ultraviolet and cobalt-60 radiations. Science. 1967 Aug 11;157(3789):703–705. doi: 10.1126/science.157.3789.703. [DOI] [PubMed] [Google Scholar]
  3. Duff R., Knight P., Rapp F. Variation in oncogenic and transforming potential of PARA (defective SV40)-adenovirus 7. Virology. 1972 Mar;47(3):849–853. doi: 10.1016/0042-6822(72)90579-x. [DOI] [PubMed] [Google Scholar]
  4. GREEN M., PINA M. Biochemical studies on adenovirus multiplication. IV. Isolation, purification, and chemical analysis of adenovirus. Virology. 1963 May;20:199–207. doi: 10.1016/0042-6822(63)90157-0. [DOI] [PubMed] [Google Scholar]
  5. Green M. Oncogenic viruses. Annu Rev Biochem. 1970;39:701–756. doi: 10.1146/annurev.bi.39.070170.003413. [DOI] [PubMed] [Google Scholar]
  6. Lawrence W. C., Ginsberg H. S. Intracellular uncoating of type 5 adenovirus deoxyribonucleic acid. J Virol. 1967 Oct;1(5):851–867. doi: 10.1128/jvi.1.5.851-867.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mak S. Defective virions in human adenovirus type 12. J Virol. 1971 Apr;7(4):426–433. doi: 10.1128/jvi.7.4.426-433.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mak S., Mak I. Viral DNA synthesis in cells infected with ultraviolet-irradiated human adenovirus type 2. Biochim Biophys Acta. 1974 Mar 8;340(2):117–129. doi: 10.1016/0005-2787(74)90104-x. [DOI] [PubMed] [Google Scholar]
  9. Nashimoto H., Uchida H. Indole as an activator for in vitro attachment of tail fibers in the assembly of bacteriophage T4D. Virology. 1969 Jan;37(1):1–7. doi: 10.1016/0042-6822(69)90299-2. [DOI] [PubMed] [Google Scholar]
  10. POPE J. H., ROWE W. P. IMMUNOFLUORESCENT STUDIES OF ADENOVIRUS 12 TUMORS AND OF CELLS TRANSFORMED OR INFECTED BY ADENOVIRUSES. J Exp Med. 1964 Oct 1;120:577–588. doi: 10.1084/jem.120.4.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rainbow A. J., Mak S. Functional heterogeneity of virions in human adenovirus types 2 and 12. J Virol. 1970 Feb;5(2):188–193. doi: 10.1128/jvi.5.2.188-193.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shimojo H., Yamashita T. Induction of DNA synthesis by adenoviruses in contact-inhibited hamster cells. Virology. 1968 Nov;36(3):422–433. doi: 10.1016/0042-6822(68)90167-0. [DOI] [PubMed] [Google Scholar]
  13. Sitch H. F., Yohn D. S. Viruses and chromosomes. Prog Med Virol. 1970;12:78–127. [PubMed] [Google Scholar]
  14. Strohl W. A. The response of BHK21 cells to infection with type 12 adenovirus. 1. Cell killing and T antigen synthesis as correlated viral genome functions. Virology. 1969 Dec;39(4):642–652. doi: 10.1016/0042-6822(69)90003-8. [DOI] [PubMed] [Google Scholar]
  15. Takemori N., Riggs J. L., Aldrich C. D. Genetic studies with tumorigenic adenoviruses. II. Heterogeneity of cyt mutants of adenovirus type 12. Virology. 1969 May;38(1):8–15. doi: 10.1016/0042-6822(69)90122-6. [DOI] [PubMed] [Google Scholar]
  16. Takemori N., Riggs J. L., Aldrich C. Genetic studies with tumorigenic adenoviruses. I. Isolation of cytocidal (cyt) mutants of adenovirus type 12. Virology. 1968 Dec;36(4):575–586. doi: 10.1016/0042-6822(68)90189-x. [DOI] [PubMed] [Google Scholar]
  17. Uchida S., Watanabe S. Transformation of mouse 3T3 cells by T antigen-forming defective SV40 virions (T particles). Virology. 1969 Dec;39(4):721–728. doi: 10.1016/0042-6822(69)90009-9. [DOI] [PubMed] [Google Scholar]
  18. Yamamoto H., Shimojo H., Hamada C. Less tumorigenic (cyt) mutants of adenovirus 12 defective in induction of cell surface change. Virology. 1972 Dec;50(3):743–752. doi: 10.1016/0042-6822(72)90428-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES