Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1974 Oct;14(4):1017–1021. doi: 10.1128/jvi.14.4.1017-1021.1974

Quantitation of Endogenous C-type Virion Production in Several Murine Cell Lines

Ronald B Luftig 1, Paul N Mcmillan 1, Mollie Gudger 1
PMCID: PMC355610  PMID: 4371663

Abstract

Quantitation by enumeration of virion particles, measurement of absorbancy at 260 nm, and densitometry on sodium dodecyl sulfate-polyacrylamide gels has shown that mouse L-M cells yielded 7- to 10-fold less endogenous C-type virions than the parental lines, L or L929. The previously noted stimulation of L-M cell virion production by a concomitant 10% increase in fetal calf serum concentration (D. A. Kindig, R. Karp, and W. H. Kirsten, 1968), was not observed.

Full text

PDF
1020

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DALES S., HOWATSON A. F. Virus-like particles in association with L strain cells. Cancer Res. 1961 Feb;21:193–197. [PubMed] [Google Scholar]
  2. Kalter S. S., Helmke R. J., Panigel M., Heberling R. L., Felsburg P. J., Axelrod L. R. Observations of apparent C-type particles in baboon (Papio cynocephalus) placentas. Science. 1973 Mar 30;179(4080):1332–1333. doi: 10.1126/science.179.4080.1332. [DOI] [PubMed] [Google Scholar]
  3. Kindig D. A., Karp R., Kirsten W. H. Further characterization of L-cell virions. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1103–1109. doi: 10.1073/pnas.59.4.1103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kindig D. A., Kirsten W. H. Virus-like particles in established murine cell lines: electron-microscopic observations. Science. 1967 Mar 24;155(3769):1543–1545. doi: 10.1126/science.155.3769.1543. [DOI] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Lieber M. M., Benveniste R. E., Livingston D. M., Todaro G. J. Mammalian cells in culture frequently release type C viruses. Science. 1973 Oct 5;182(4107):56–59. doi: 10.1126/science.182.4107.56. [DOI] [PubMed] [Google Scholar]
  7. Lowy D. R., Rowe W. P., Teich N., Hartley J. W. Murine leukemia virus: high-frequency activation in vitro by 5-iododeoxyuridine and 5-bromodeoxyuridine. Science. 1971 Oct 8;174(4005):155–156. doi: 10.1126/science.174.4005.155. [DOI] [PubMed] [Google Scholar]
  8. Nichols J. L., Quade K., Luftig R. B. Physiocochemical studies on L-cell virions. J Virol. 1973 Mar;11(3):432–440. doi: 10.1128/jvi.11.3.432-440.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sethi K. K., Pelster B., Brandis H. Cytochalasin B-induced activation in the synthesis of L-cell virus particles. J Gen Virol. 1973 Nov;21(2):435–440. doi: 10.1099/0022-1317-21-2-435. [DOI] [PubMed] [Google Scholar]
  10. Smith R. E., Bernstein E. H. Production and purification of large amounts of Rous sarcoma virus. Appl Microbiol. 1973 Mar;25(3):346–353. doi: 10.1128/am.25.3.346-353.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Weinstein I. B., Gebert R., Stadler U. C., Orenstein J. M., Axel R. Type C virus from cell cultures of chemically induced rat hepatomas. Science. 1972 Dec 8;178(4065):1098–1100. doi: 10.1126/science.178.4065.1098. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES