Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1975 Nov;16(5):1229–1235. doi: 10.1128/jvi.16.5.1229-1235.1975

Biochemical and biophysical characteristics of diarrhea viruses of human and calf origin.

S M Rodger, R D Schnagl, I H Holmes
PMCID: PMC355722  PMID: 171450

Abstract

Polyacrylamide gel electrophoretic analysis of purified preparations of human and calf diarrhea viruses indicated eight polypeptide components, or possibly nine in the case of the calf diarrhea virus. Thermal denaturation and analytical studies of the calf diarrhea virus genome showed it to consist of 11 double-stranded segments of RNA. The placing of the human and calf diarrhea viruses together with other similar viruses into a genus separate from reovirus and orbivirus, but within the family Reoviridae, is discussed.

Full text

PDF
1233

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Banfield W. G., Kasnic G., Blackwell J. H. Further observations on the virus of epizootic diarrhea of infant mice. An electron microscopic study. Virology. 1968 Nov;36(3):411–421. doi: 10.1016/0042-6822(68)90166-9. [DOI] [PubMed] [Google Scholar]
  3. Bellamy A. R., Shapiro L., August J. T., Joklik W. K. Studies on reovirus RNA. I. Characterization of reovirus genome RNA. J Mol Biol. 1967 Oct 14;29(1):1–17. doi: 10.1016/0022-2836(67)90177-5. [DOI] [PubMed] [Google Scholar]
  4. Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. Detection of a new virus by electron microscopy of faecal extracts from children with acute gastroenteritis. Lancet. 1974 Feb 2;1(7849):149–151. doi: 10.1016/s0140-6736(74)92440-4. [DOI] [PubMed] [Google Scholar]
  5. Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis. Lancet. 1973 Dec 8;2(7841):1281–1283. doi: 10.1016/s0140-6736(73)92867-5. [DOI] [PubMed] [Google Scholar]
  6. Davidson G. P., Bishop R. F., Townley R. R., Holmes I. H. Importance of a new virus in acute sporadic enteritis in children. Lancet. 1975 Feb 1;1(7901):242–246. doi: 10.1016/s0140-6736(75)91140-x. [DOI] [PubMed] [Google Scholar]
  7. Els H. J., Lecatsas G. Morphological studies on simian virus S.A. 11 and the 'related' O agent. J Gen Virol. 1972 Oct;17(1):129–132. doi: 10.1099/0022-1317-17-1-129. [DOI] [PubMed] [Google Scholar]
  8. Flewett T. H., Bryden A. S., Davies H. Letter: Virus particles in gastroenteritis. Lancet. 1973 Dec 29;2(7844):1497–1497. doi: 10.1016/s0140-6736(73)92760-8. [DOI] [PubMed] [Google Scholar]
  9. Flewett T. H., Bryden A. S., Davies H., Woode G. N., Bridger J. C., Derrick J. M. Relation between viruses from acute gastroenteritis of children and newborn calves. Lancet. 1974 Jul 13;2(7872):61–63. doi: 10.1016/s0140-6736(74)91631-6. [DOI] [PubMed] [Google Scholar]
  10. Fujii-Kawata I., Miura K. I. Segments of genome of viruses containing double-stranded ribonucleic acid. J Mol Biol. 1970 Jul 28;51(2):247–253. doi: 10.1016/0022-2836(70)90140-3. [DOI] [PubMed] [Google Scholar]
  11. Kapikian A. Z., Kim H. W., Wyatt R. G., Rodriguez W. J., Ross S., Cline W. L., Parrott R. H., Chanock R. M. Reoviruslike agent in stools: association with infantile diarrhea and development of serologic tests. Science. 1974 Sep 20;185(4156):1049–1053. doi: 10.1126/science.185.4156.1049. [DOI] [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Loening U. E. The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation. Biochem J. 1969 Jun;113(1):131–138. doi: 10.1042/bj1130131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Loh P. C., Shatkin A. J. Structural proteins of reoviruses. J Virol. 1968 Nov;2(11):1353–1359. doi: 10.1128/jvi.2.11.1353-1359.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Martin S. A., Zweerink H. J. Isolation and characterization of two types of bluetongue virus particles. Virology. 1972 Nov;50(2):495–506. doi: 10.1016/0042-6822(72)90400-x. [DOI] [PubMed] [Google Scholar]
  16. Mebus C. A., Kono M., Underdahl N. R., Twiehaus M. J. Cell culture propagation of neonatal calf diarrhea (scours) virus. Can Vet J. 1971 Mar;12(3):69–72. [PMC free article] [PubMed] [Google Scholar]
  17. Melnick J. L. Classification and nomenclature of viruses, 1973. Prog Med Virol. 1973;16:337–342. [PubMed] [Google Scholar]
  18. Nonoyama M., Watanabe Y., Graham A. F. Defective virions of reovirus. J Virol. 1970 Aug;6(2):226–236. doi: 10.1128/jvi.6.2.226-236.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Oliver D., Chalkley R. An improved photographic system for polyacrylamide gels. Anal Biochem. 1971 Dec;44(2):540–542. doi: 10.1016/0003-2697(71)90242-9. [DOI] [PubMed] [Google Scholar]
  20. Reddy D. V., Black L. M. Electrophoretic separation of all components of the double-stranded RNA of wound tumor virus. Virology. 1973 Aug;54(2):557–562. doi: 10.1016/0042-6822(73)90168-2. [DOI] [PubMed] [Google Scholar]
  21. Shatkin A. J., Sipe J. D., Loh P. Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis. J Virol. 1968 Oct;2(10):986–991. doi: 10.1128/jvi.2.10.986-991.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Smith R. E., Zweerink H. J., Joklik W. K. Polypeptide components of virions, top component and cores of reovirus type 3. Virology. 1969 Dec;39(4):791–810. doi: 10.1016/0042-6822(69)90017-8. [DOI] [PubMed] [Google Scholar]
  23. Turner A. J., Caple I. W., Craven J. A., Reinganum C. Letter: Demonstration of virus particles in intestinal contents of calves with diarrhoea. Aust Vet J. 1973 Nov;49(11):544–544. doi: 10.1111/j.1751-0813.1973.tb02354.x. [DOI] [PubMed] [Google Scholar]
  24. Verwoerd D. W., Els H. J., De Villiers E. M., Huismans H. Structure of the bluetongue virus capsid. J Virol. 1972 Oct;10(4):783–794. doi: 10.1128/jvi.10.4.783-794.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Verwoerd D. W., Louw H., Oellermann R. A. Characterization of bluetongue virus ribonucleic acid. J Virol. 1970 Jan;5(1):1–7. doi: 10.1128/jvi.5.1.1-7.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Welch A. B. Purification, morphology and partial characterization of a reovirus-like agent associated with neonatal calf diarrhea. Can J Comp Med. 1971 Jul;35(3):195–202. [PMC free article] [PubMed] [Google Scholar]
  27. Welch A. B., Thompson T. L. Physiochemical characterization of a neonatal calf diarrhea virus. Can J Comp Med. 1973 Jul;37(3):295–301. [PMC free article] [PubMed] [Google Scholar]
  28. Woode G. N., Bridger J. C., Hall G., Dennis M. J. The isolation of a reovirus-like agent associated with diarrhoea in colostrum-deprived calves in Great Britain. Res Vet Sci. 1974 Jan;16(1):102–105. [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES