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. 1971 Feb;7(2):198–203. doi: 10.1128/jvi.7.2.198-203.1971

Structural Proteins of Simian Virus 40

Stanley Barban 1, Ronald S Goor 1
PMCID: PMC356098  PMID: 4329397

Abstract

Sodium dodecyl sulfate acrylamide gel electrophoresis of the solubilized proteins from purified simian virus 40 (SV40) virions revealed two major and two minor structural polypeptide components. The major components which comprise over 75% of the total virion were shown to be the capsid proteins by immunological and isoelectric focusing fractionation analysis. These two polypeptides have estimated molecular weights of 45,000 daltons as determined by gel electrophoresis. One of the two minor components was identified as the nucleocapsid protein and has an approximate molecular weight of 16,000. The other unidentified minor component has an average molecular weight of 29,000.

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Selected References

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  1. Anderer F. A., Koch M. A., Schlumberger H. D. Structure of simian virus 40. 3. Alkaline degradation of the virus particle. Virology. 1968 Mar;34(3):452–458. doi: 10.1016/0042-6822(68)90065-2. [DOI] [PubMed] [Google Scholar]
  2. Anderer F. A., Schlumberger H. D., Koch M. A., Frank H., Eggers H. J. Structure of simian virus 40. II. Symmetry and components of the virus particle. Virology. 1967 Jul;32(3):511–523. doi: 10.1016/0042-6822(67)90303-0. [DOI] [PubMed] [Google Scholar]
  3. BLACK P. H., CRAWFORD E. M., CRAWFORD L. V. THE PURIFICATION OF SIMIAN VIRUS 40. Virology. 1964 Nov;24:381–387. doi: 10.1016/0042-6822(64)90175-8. [DOI] [PubMed] [Google Scholar]
  4. BLACK P. H., ROWE W. P., TURNER H. C., HUEBNER R. J. A SPECIFIC COMPLEMENT-FIXING ANTIGEN PRESENT IN SV40 TUMOR AND TRANSFORMED CELLS. Proc Natl Acad Sci U S A. 1963 Dec;50:1148–1156. doi: 10.1073/pnas.50.6.1148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Girard M., Marty L., Suarez F. Capsid proteins of Simian virus 40. Biochem Biophys Res Commun. 1970 Jul 13;40(1):97–102. doi: 10.1016/0006-291x(70)91051-x. [DOI] [PubMed] [Google Scholar]
  6. HABEL K., EDDY B. E. Specificity of resistance to tumor challenge of polyoma and SV 40 virus-immune hamsters. Proc Soc Exp Biol Med. 1963 May;113:1–4. doi: 10.3181/00379727-113-28259. [DOI] [PubMed] [Google Scholar]
  7. Kit S., Dubbs D. R., Frearson P. M., Melnick J. L. Enzyme induction in SV40-infected green monkey kidney cultures. Virology. 1966 May;29(1):69–83. doi: 10.1016/0042-6822(66)90197-8. [DOI] [PubMed] [Google Scholar]
  8. Kit S., Piekarski L. J., Dubbs D. R. DNA polymerase induced by Simian virus 40. J Gen Virol. 1967 Apr;1(2):163–173. doi: 10.1099/0022-1317-1-2-163. [DOI] [PubMed] [Google Scholar]
  9. Koch M. A., Eggers H. J., Anderer F. A., Schlumberger H. D., Frank H. Structure of simian virus 40. I. Purification and physical characterization of the virus particle. Virology. 1967 Jul;32(3):503–510. doi: 10.1016/0042-6822(67)90302-9. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Maizel J. V., Jr, White D. O., Scharff M. D. The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12. Virology. 1968 Sep;36(1):115–125. doi: 10.1016/0042-6822(68)90121-9. [DOI] [PubMed] [Google Scholar]
  12. Moss B., Salzman N. P. Sequential protein synthesis following vaccinia virus infection. J Virol. 1968 Oct;2(10):1016–1027. doi: 10.1128/jvi.2.10.1016-1027.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schlumberger H. D., Anderer F. A., Koch M. A. Structure of simian virus 40. IV. The polypeptide chains of the virus particle. Virology. 1968 Sep;36(1):42–47. doi: 10.1016/0042-6822(68)90114-1. [DOI] [PubMed] [Google Scholar]
  14. Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]
  15. Summers D. F., Maizel J. V., Jr, Darnell J. E., Jr Evidence for virus-specific noncapsid proteins in poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1965 Aug;54(2):505–513. doi: 10.1073/pnas.54.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. TEVETHIA S. S., KATZ M., RAPP F. NEW SURFACE ANTIGEN IN CELLS TRANSFORMED BY SIMIAN PAPOVAVIRUS SV40. Proc Soc Exp Biol Med. 1965 Jul;119:896–901. doi: 10.3181/00379727-119-30330. [DOI] [PubMed] [Google Scholar]
  17. Tevethia S. S., Diamandopoulos G. T., Rapp F., Enders J. F. Lack of relationship between virus-specific surface and transplantation antigens in hamster cells transformed by simian papovavirus SV40. J Immunol. 1968 Dec;101(6):1192–1198. [PubMed] [Google Scholar]
  18. Todaro G. J., Takemoto K. K. "Rescued" SV40: increased transforming efficiency in mouse and human cells. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1031–1037. doi: 10.1073/pnas.62.4.1031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Vesterberg O., Svensson H. Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients. IV. Further studies on the resolving power in connection with separation of myoglobins. Acta Chem Scand. 1966;20(3):820–834. doi: 10.3891/acta.chem.scand.20-0820. [DOI] [PubMed] [Google Scholar]

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