Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1971 Apr;7(4):515–523. doi: 10.1128/jvi.7.4.515-523.1971

Abortive Infection of Sporulating Bacillus subtilis 168 by φ2 Bacteriophage

Junetsu Ito 1, John Spizizen 1
PMCID: PMC356152  PMID: 5000116

Abstract

Bacteriophage φ2 is unable to replicate in Bacillus subtilis 168. Although some phage deoxyribonucleic acid (DNA) synthesis can occur, the DNA made is not biologically active and sedimentation analysis reveals that it is smaller in size than that of mature DNA or DNA isolated from φ2-infected permissive hosts. Messenger ribonucleic acid hybridizable with φ2 DNA is also synthesized in φ2-infected cells of 168. Mutants of 168 which are permissive hosts for φ2 have been isolated. These mutants are defective in sporulation and possess the phenotype of “early sporulation mutants.” The majority map in two locations, one near the lys locus opposite the trp locus (spoA locus) and the other tightly linked to a phe locus.

Full text

PDF
521

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alegria A. H., Kahan F. M., Marmur J. A new assay for phage hydroxymethylases and its use in Bacillus subtilis transfection. Biochemistry. 1968 Sep;7(9):3179–3186. doi: 10.1021/bi00849a021. [DOI] [PubMed] [Google Scholar]
  2. Arber W., Linn S. DNA modification and restriction. Annu Rev Biochem. 1969;38:467–500. doi: 10.1146/annurev.bi.38.070169.002343. [DOI] [PubMed] [Google Scholar]
  3. Borenstein S., Ephrati-Elizur E. Spontaneous release of DNA in sequential genetic order by Bacillus subtilis. J Mol Biol. 1969 Oct 14;45(1):137–152. doi: 10.1016/0022-2836(69)90216-2. [DOI] [PubMed] [Google Scholar]
  4. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  5. FUKASAWA T. THE COURSE OF INFECTION WITH ABNORMAL BACTERIOPHAGE T4 CONTAINING NON-GLUCOSYLATED DNA ON ESCHERICHIA COLI STRAINS. J Mol Biol. 1964 Aug;9:525–536. doi: 10.1016/s0022-2836(64)80224-2. [DOI] [PubMed] [Google Scholar]
  6. Frankel F. R., Majumdar C., Weintraub S., Frankel D. M. DNA polymerase and the cell membrane after T4 infection. Cold Spring Harb Symp Quant Biol. 1968;33:495–500. doi: 10.1101/sqb.1968.033.01.057. [DOI] [PubMed] [Google Scholar]
  7. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  8. Goldberg I. D., Bryan T. Productive infection of Bacillus subtilis 168, with bacteriophage SP-10, dependent upon inducing treatments. J Virol. 1968 Aug;2(8):805–812. doi: 10.21236/ad0686354. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hattman S., Revel H. R., Luria S. E. Enzyme synthesis directed by nonglucosylated T-even bacteriophages in restrictive hosts. Virology. 1966 Nov;30(3):427–438. doi: 10.1016/0042-6822(66)90120-6. [DOI] [PubMed] [Google Scholar]
  10. Hausmann R., Gomez B., Moody B. Physiological and genetic aspects of abortive infection of a Shigella sonnei strain by coliphage T7. J Virol. 1968 Apr;2(4):335–345. doi: 10.1128/jvi.2.4.335-345.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hoch J. A., Barat M., Anagnostopoulos C. Transformation and transduction in recombination-defective mutants of Bacillus subtilis. J Bacteriol. 1967 Jun;93(6):1925–1937. doi: 10.1128/jb.93.6.1925-1937.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ionesco H., Michel J., Cami B., Schaeffer P. Symposium on bacterial spores: II. Genetics of sporulation in Bacillus subtilis Marburg. J Appl Bacteriol. 1970 Mar;33(1):13–24. doi: 10.1111/j.1365-2672.1970.tb05230.x. [DOI] [PubMed] [Google Scholar]
  13. Jones O. W., Dieckmann M., Berg P. Ribosome-induced dissociation of RNA from an RNA polymerase-DNA-RNA complex. J Mol Biol. 1968 Jan 28;31(2):177–189. doi: 10.1016/0022-2836(68)90438-5. [DOI] [PubMed] [Google Scholar]
  14. Losick R., Sonenshein A. L. Change in the template specificity of RNA polymerase during sporulation of Bacillus subtilis. Nature. 1969 Oct 4;224(5214):35–37. doi: 10.1038/224035a0. [DOI] [PubMed] [Google Scholar]
  15. MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
  16. Mathews C. K. T-even bacteriophage-tolerant mutants of Escherichia coli B. I. Isolation and preliminary characterization. J Virol. 1970 Aug;6(2):163–168. doi: 10.1128/jvi.6.2.163-168.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McCarthy C., Nester E. W. Heat-activated endonuclease in Bacillus subtilis. J Bacteriol. 1969 Mar;97(3):1426–1430. doi: 10.1128/jb.97.3.1426-1430.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Miller R. C., Jr, Buckley P. Early intracellular events in the replication of bacteriophage T4 deoxyribonucleic acid. VI. Newly synthesized proteins in the T4 protein-deoxyribonucleic acid complex. J Virol. 1970 Apr;5(4):502–506. doi: 10.1128/jvi.5.4.502-506.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. OKAMOTO K., SUGINO Y., NOMURA M. Synthesis and turnover of phage messenger RNA in E. coli infected with bacteriophage T4 in the presence of chloromycetin. J Mol Biol. 1962 Nov;5:527–534. doi: 10.1016/s0022-2836(62)80126-0. [DOI] [PubMed] [Google Scholar]
  20. REILLY B. E., SPIZIZEN J. BACTERIOPHAGE DEOXYRIBONUCLEATE INFECTION OF COMPETENT BACILLUS SUBTILIS. J Bacteriol. 1965 Mar;89:782–790. doi: 10.1128/jb.89.3.782-790.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rogolsky M. Chromosomal regions which control sporulation in Bacillus subtilis. Can J Microbiol. 1969 Jul;15(7):787–790. doi: 10.1139/m69-137. [DOI] [PubMed] [Google Scholar]
  22. SEKIGUCHI M., TAKAGI Y. Effect of mitomycin C on the synthesis of bacterial and viral deoxyribonucleic acid. Biochim Biophys Acta. 1960 Jul 15;41:434–443. doi: 10.1016/0006-3002(60)90040-8. [DOI] [PubMed] [Google Scholar]
  23. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  24. Sonenshein A. L., Losick R. RNA polymerase mutants blocked in sporulation. Nature. 1970 Aug 29;227(5261):906–909. doi: 10.1038/227906a0. [DOI] [PubMed] [Google Scholar]
  25. Sonenshein A. L., Roscoe D. H. The course of phage phi-e infection in sporulating cells of Bacillus subtilis strain 3610. Virology. 1969 Oct;39(2):265–275. doi: 10.1016/0042-6822(69)90047-6. [DOI] [PubMed] [Google Scholar]
  26. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. TAKAHASHI I. Genetic transformation of Bacillus subtilis by extracellular DNA. Biochem Biophys Res Commun. 1962 Jun 4;7:467–470. doi: 10.1016/0006-291x(62)90337-6. [DOI] [PubMed] [Google Scholar]
  28. TAKAHASHI I. TRANSDUCTION OF SPOROGENESIS IN BACILLUS SUBTILIS. J Bacteriol. 1965 Feb;89:294–298. doi: 10.1128/jb.89.2.294-298.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. TAKAHASHI I. Transducing phages for Bacillus subtilis. J Gen Microbiol. 1963 May;31:211–217. doi: 10.1099/00221287-31-2-211. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES