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. 1971 Jul;8(1):74–80. doi: 10.1128/jvi.8.1.74-80.1971

Transcription of the Influenza Ribonucleic Acid Genome by a Virion Polymerase II. Nature of the In Vitro Polymerase Product

David H L Bishop 1,2, John F Obijeski 1,2, Robert W Simpson 1,2
PMCID: PMC356216  PMID: 5106347

Abstract

The properties of the ribonucleic acid (RNA) synthesized by the influenza (WSN) virion polymerase have been investigated. Most of the product RNA is synthesized in association with virion RNA species from which it can be released by heat treatment as single-stranded, ribonuclease-sensitive polynucleotides (average molecular weight, 2-hr sample, about 105 daltons). At least 95% of the product is complementary to the viral RNA species. On the basis of the molar ratio of the RNA species isolated from a 3H-uridine-labeled virus preparation, it was calculated that the WSN genome consists of seven pieces of RNA with a sum molecular weight of about 5 × 106 daltons.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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