Abstract
Sendai virions contain an enzyme which catalyzes the incorporation of ribonucleotides into ribonucleic acid (RNA). Enzyme activity was optimal at pH 8.0 and 28 C; otherwise conditions were similar to those reported for Newcastle disease virion (NDV) RNA polymerase. The initial rate of RNA synthesis by the Sendai virion enzyme was about 10 pmoles per mg of protein per hr, but after 3 hr of incubation the rate increased about fivefold. The virion enzyme was compared with an RNA polymerase in the microsomal fraction of infected cells. Both enzymes made predominantly single-stranded RNA which was complementary in base sequences to 50S virion RNA. Most of the RNA synthesized by the virion polymerase sedimented at 16S, but the product of the microsomal enzyme sedimented at about 8S.
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Selected References
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- Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blair C. D., Robinson W. S. Replication of Sendai virus. I. Comparison of the viral RNA and virus-specific RNA synthesis with Newcastle disease virus. Virology. 1968 Aug;35(4):537–549. doi: 10.1016/0042-6822(68)90284-5. [DOI] [PubMed] [Google Scholar]
- Darlington R. W., Portner A., Kingsbury D. W. Sendai virus replication: an ultrastructural comparison of productive and abortive infections in avian cells. J Gen Virol. 1970 Dec;9(3):169–177. doi: 10.1099/0022-1317-9-3-169. [DOI] [PubMed] [Google Scholar]
- Erikson R. L., Fenwick M. L., Franklin R. M. Replication of bacteriophage RNA: some properties of the parental-labeled replicative intermediate. J Mol Biol. 1965 Sep;13(2):399–406. doi: 10.1016/s0022-2836(65)80105-x. [DOI] [PubMed] [Google Scholar]
- Huang A. S., Baltimore D., Bratt M. A. Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase. J Virol. 1971 Mar;7(3):389–394. doi: 10.1128/jvi.7.3.389-394.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kingsbury D. W. Newcastle disease virus RNA. I. Isolation and preliminary characterization of RNA from virus particles. J Mol Biol. 1966 Jun;18(1):195–203. doi: 10.1016/s0022-2836(66)80085-2. [DOI] [PubMed] [Google Scholar]
- Kingsbury D. W. Newcastle disease virus RNA. II. Preferential synthesis of RNA complementary to parental viral RNA by chick embryo cells. J Mol Biol. 1966 Jun;18(1):204–214. doi: 10.1016/s0022-2836(66)80086-4. [DOI] [PubMed] [Google Scholar]
- Kingsbury D. W., Portner A., Darlington R. W. Properties of incomplete Sendai virions and subgenomic viral RNAs. Virology. 1970 Dec;42(4):857–871. doi: 10.1016/0042-6822(70)90335-1. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Mahy B. W., Hutchinson J. E., Barry R. D. Ribonucleic acid polymerase induced in cells infected with Sendai virus. J Virol. 1970 Jun;5(6):663–671. doi: 10.1128/jvi.5.6.663-671.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Penman S. RNA metabolism in the HeLa cell nucleus. J Mol Biol. 1966 May;17(1):117–130. doi: 10.1016/s0022-2836(66)80098-0. [DOI] [PubMed] [Google Scholar]
- Portner A., Kingsbury D. W. Complementary RNAs in paramyxovirions and paramyxovirus-infected cells. Nature. 1970 Dec 19;228(5277):1196–1197. doi: 10.1038/2281196a0. [DOI] [PubMed] [Google Scholar]
- Robinson W. S. Self-annealing of subgroup 2 myxovirus RNAs. Nature. 1970 Mar 7;225(5236):944–945. doi: 10.1038/225944a0. [DOI] [PubMed] [Google Scholar]